Services
- High-throughput screening of small molecules. Projects can use our own in-house collections (total of 75,000 compounds) or those supplied by the customer
- Assay development assistance
- Data analysis and development of custom analysis tools
- Data management
- Computational chemistry services offered via the Bergen NOR-Openscreen node (only for academic users). Visit: Computational chemistry services (uib.no/) for more details.
Prices
Price list (pdf) for the High-Throughput Chemical Biology Screening Platform
Technologies
Detection Platforms
- BioTek Synergy™ Neo2 Multi-Mode Microplate Reader
- Filter and monochromator based detection, stacker, barcode reader, 2 channel dispenser
- AlphaScreen™, luminescence, fluorescence intensity (FI), fluorescence polarization (FP), time-resolved fluorescence (TRF), UV-visible absorbance
- PerkinElmer EnVision®
- Filter and monochromator based detection, stacker, barcode reader, 2 channel dispenser
- AlphaScreen™, luminescence, fluorescence intensity (FI), time-resolved fluorescence (TRF), absorbance
- IntelliCyt iQue Screener PLUS
- High throughput flow cytometer 96- and 384-well format
- 3 lasers (violet 405 nm with 6 detectors, blue 488 nm with 5 detectors and red 640 nm with 2 detectors)
- Multiparameter analysis of cell surface markers, cytokines, transcription factors and phosphoproteins and cell cycle analysis
Liquid Handling
- LABCYTE Access™ Laboratory Workstation integrating:
- LABCYTE Echo 650 acoustic dispenser (accurate tip-free dispensing of compounds, DNA, antibodies, reagents, proteins, peptides etc. as low as 2.5 nL to and from any well of microplates)
- PerkinElmer EnVision® 2104 Multilabel Plate Reader
- BioTek MultiFlo FX dispenser/washer
- Thermo Fisher Scientific Multidrop Combi nL dispenser
- Thermo Fisher Scientific Cytomat Cell Incubator
- Agilent VSpin Plate centrifuge
- Agilent PlateLoc Thermal Microplate Sealer
- Brooks FluidX® XPeel® Automated Microplate Peeler
-
Beckman Coulter Biomek i7
- Liquid handling robot with 96/384 exchangeable multichannel pipetting head and 8 single channels
- Integrated Agilent microplate centrifuge
- BioTek EL406 plate washer and dispenser with BioStack 4 Microplate Stacker
- PerkinElmer FlexDrop Exi dispenser
Compound storage
- Roylan StoragePod
- MultiPod with 2 “San Francisco” and 3 “Short Dundee” pods
- Humidity controlled nitrogen atmosphere
Other Instrumentation
- Eppendorf microplate centrifuge
- Barcode printing and reading
Informatics
Data analysis and development of customized solutions is performed using R, Python and KNIME. Data analysis we normally offer (but not limited to):
- Quality Control of HTS data
- Hit identification.
- Dose response analysis
- Custom plate layouts
- Statistical Analysis (Descriptive analysis, PCA, Regression, clustering etc)
- Data cleaning, wrangling, summaries and interactive visualizations
Additionally, we offer customized computational solutions that are tailored to the user needs unmet by already available software. Whether you need assistance with bioinformatics analysis or have a project with a special computational need, we can help. We use Instant JChem (Chemaxon) for the management of our compound collections. For file transfer we use UiO FileSender for open and restricted data.
Compounds
Diverse Collections
(Chemically diverse drug-like molecules)
- Enamine (28160 compounds)
- ChemBridge (~17501 compounds)
- ChemDiv (~16671 compounds)
Drug Repurposing Collections
- Prestwick Chemical Library, FDA/EMA Approved Drugs (1520 compounds)
- MedChemExpress, FDA/EMA Approved Drugs (860 compounds)
- ChemDiv, FDA/EMA Approved Drugs (170 compounds)
- SelleckChem, FDA/EMA Approved Drugs (867 compounds)
- SelleckChem Oncoset, Oncology-Related Compounds (433 compounds)
- SelleckChem, Cambridge Cancer Compound Library (384 compounds)
- SelleckChem, Bioactives (partly FDA and phase I) (991 compounds)
Other Targeted and Bioactives
- Sigma, LOPAC, Pharmacologically Active (1280 compounds)
- Prestwick, POM, Original Molecules (344 compounds)
- SelleckChem, Tyrosine Kinase Inhibitors L1800 (170 compounds)
- SelleckChem, Kinase Inhibitor L1200 (193 compounds)
- BioMol, Kinase Inhibitors and Bioactives (159 compounds)
- Tocris, Tocriscreen Mini, Bioactives (1122 compounds)
- Enzo, Target and Pathway Libraries (531 compounds)
- Asinex, Protein-Protein Interaction (1008 compounds)
- MedChemExpress, Epigenetics Library (2187 compounds)
Contact information
Booking
Email: chembio@ncmm.uio.no
Management
Johannes Landskron
Head of Facility
Phone: +47 22 84 05 09
Alexandra Gade
Senior Engineer
Phone: +47 22 84 41 30
Eirin Solberg
Head Engineer
Phone: +47 22 84 05 13
Amna Farooq (OUS)
Special Engineer, Bioinformatician
Phone: +47 22 84 58 56 (Mon,Tue,Thu)
Can also be reached at:
Phone: +47 91 83 34 25 (Wed, Fri)
Bioinformatics/Genomics Core facility,
7th Floor, New OCCI building (Blue),
Norwegian Radium Hospital, Ullernchausséen 66, 0379, Oslo
Visiting address
Building E, 3rd floor
Forskningsparken
Gaustadalléen 21
0349 Oslo