Introduction and considerations
When working with genetically modified animals, it is absolutely crucial to have available a simple method for DNA analysis to identify the genetic modification. The most commonly used approach that will work in most cases will be a PCR-based strategy.
Description of the service
- NTS may perform genotyping on a regular basis as a service.
- NTS may then adapt a method developed by the customer provided that the method is able to detect a single copy in the genome.
- NTS may improve an existing screening method and replace it with a better method, specific for the modification.
- NTS can develop a new screening method that is specific for the modification.
Requirements and time-frame
- The customer must provide exact nucleotide sequence(s) for the region(s) containing the genetic modification.
- The design and validation is typically performed within 1-2 weeks.
Costs cover the following
- Technical time to establish a new genotyping strategy.
- Ordering of primers.
- Validation of primer specificity.
Conditions
- Costs are for design of regular PCR based strategies.
- Depending on the nature and the extent of the work involved, advice may be charged an hourly salary.
Service Fees
Estimated costs for the above service can be found on our Service Fees page.