Contact us at transgenic@basalmed.uio.no and we will get back to you with additional information.
The table below summarizes minimum costs for different project categories.
For cost details and type of service, see further down.
| Service category | IMB (UiO) and OUS | Other Academic | Non-academic |
|---|---|---|---|
| Technical assistance | 750 * | 1000 * | 2000 * |
| Cloning - regular | Enquire | Enquire | Enquire |
| Cloning GateWAy | Enquire | Enquire | Enquire |
| Cloning - recombineering | Enquire | Enquire | Enquire |
| CRISPR design - CRISPR KO | 14000 | 18000 | 26000 |
| CRISPR design - CRISPR mut | 18000 | 25000 | 32000 |
| Pronuclear injection - Tg F1/129 | 75000 | 100000 | 150000 |
| Pronuclear injection - Tg bl6 | 75000 | 100000 | 150000 |
| Pronuclear injection - CRISPR KO | 65000 | 65000 | 120000 |
| Pronuclear injection - CRISPR mut | 80000 | 100000 | 150000 |
| Injection - Blastocyst | 50000 | 65000 | 120000 |
| Founder screening - Tg | 5500 | 5500 | 10000 |
| Founder screening -CRISPR | 10000 | 10000 | 18000 |
| Founder validation -CRISPR | 23000 | 26000 | 33000 |
| Cryopreservation - Embryos | 33000 | 33000 | 65000 |
| Cryopreservation - Sperm | 15000 | 15000 | 30000 |
| Rederivation - Embryos | 35000 | 35000 | 75000 |
| Animal recovery - Embryos | 15000 | 15000 | 35000 |
| Animal recovery - Sperm (IVF) | 25000 | 29000 | 36000 |
| Healt monityoring | 7500 | 7500 | 10000 |
| Genotyping - PCR (two reactions) | 100 | 130 | 200 |
| Breeding / housing | 150 ** | 200 ** | 400 ** |
* Cost pr. hour. ** Cost pr. cage pr week
Advice/technical service
| Type of service | Minimum cost (NOK) |
|---|---|
| Advice - hourly salary for demanding projects | 750 NOK/hour |
| Technical services | 750 NOK/hour |
DNA-cloning and NTS-Gateway cloning
| Type of service | Minimum cost (NOK) |
|---|---|
| Cloning | Based on project |
CRISPR design
| Type of service | Minimum cost (NOK) |
|---|---|
| Service type 1 (HNEJ - knock-out): Design sgRNAs (up to three sgRNAs) | 14.000 NOK |
| Service type 2 (HDR - insertion of mutation): Design two sgRNAs Two replacement fragments | 18.000 NOK |
| All molecular steps until final purification of working sgRNAs | Included in above |
Cryopreservation - Embryo
| Type of service | Minimum cost (NOK) |
|---|---|
| Cryopreservation where customer supplies females for superovulation (Prize excludes cage cost for donor females) | 28.000 NOK |
| C57BL/6N (Harlan & Janvier), C57BL/6J | 33.000 NOK |
| Other strains – price will be adjusted based on animal costs | >33.000 NOK |
| Cage cost housing of males | 150 NOK/cage/week |
| Cage cost offspring from 7 weeks of age | 150 NOK/cage/week |
| Storage in liquid nitrogen, distributed in two tanks | 750 NOK/strain/year (1st year included). |
Provisions: Cage cost for housing of genetically modified males will remain at the customers cost. For IMB customers males will remain under the customer’s project. For external customers this will be added to the total cost.
Cryopreservation – Sperm
| Type of service | Minimum cost (NOK) |
|---|---|
| Cryopreservation of sperm – C57BL/6 | 15.000 NOK |
| Quality control standard (QC): Test thaw – sperm motility is checked using Ceros II | Included |
| Quality control extended (QC): IVF – embryo’s in culture morula stage |
20.000 NOK |
| Immediate Quality control full (QC): IVF | 25.000 NOK* |
| Cage cost (males) | 150 NOK/cage/week |
| Cage cost offspring from 7 weeks of age | 150 NOK/cage/week |
| Storage in liquid nitrogen, distributed in two tanks | 750 NOK/year** |
| (1st year included). |
Provisions: Cage cost for housing of genetically modified males will remain at the customers cost. For IMB customers males will remain under the customer’s project. For external customers, this will be added to the total cost.
* Price for Immediate Quality Control (QC): IVF will be higher for mice with backgrounds other than C57BL/6.
** Price for continuing storage on liquid nitrogen may be adjusted yearly.
In vitro fertilization
| Type of service | Minimum cost (NOK) |
|---|---|
| IVF - C57BL/6N | 25000 NOK |
| Other strains | Inquire |
| Health certificate / pathogen testing (SFP status) | 7500 NOK * |
| Additional rounds of IVF | 15 0000 NOK |
| Separation, biopsies | Included |
| Assistance for shipment of sperm | Included |
| Cost for shipment of sperm | Payed by customer |
| Cage cost offspring from 7 weeks of age | 150 NOK/cage/week |
Provisions: *Pathogen testing (health certificate) will not be required for most projects (health monitoring will be based on the quarterly health monitoring program).
Embryo recovery
| Type of service | Minimum cost (NOK) |
|---|---|
| C57BL/6N | 15 000 NOK |
| Health certificate, mandatory health monitoring (SPF status) |
7 500 NOK |
| Additional rounds of embryo transfer | Depends on strain |
| Separation, biopsies | Included |
| Assistance for shipment of mice | Included |
| Cost for shipment of mice | Payed by customer |
| Cage cost offspring from 7 weeks of age | 150 NOK/cage/week |
Rederivation
| Type of service | Minimum cost (NOK) |
|---|---|
| C57BL/6N | 27.500 NOK |
| Other strains, depending on animal costs | >27.500 NOK |
| Health certificate, mandatory health monitoring (SFP status) |
7 500 NOK |
| Additional rounds of rederivation | Depends on strain |
| Cost for housing of males (estimate 4 males x 5 weeks) | >3000 NOK |
| Separation, biopsies | Included |
| Assistance for shipment of mice | Included |
| Cost for shipment of mice | Payed by customer |
| Cage cost offspring from 7 weeks of age | 150 NOK/cage/week |
Provisions: Cage cost for housing of genetically modified males will remain at the customers cost. For IMB customers, males will remain under the customer’s FOTS project and costs for housing of males will be charged by the animal facility.
Pathogen testing (health monitoring)
| Type of service | Minimum cost (NOK) |
|---|---|
| Pathogen screening with Health certificate of the animal colony (minimum cost) | 6 500 NOK |
| Sample collection, shipment and administrative costs | 1000 NOK |
Pronuclear injection - CRISPR
| Type of service | Minimum cost (NOK) |
|---|---|
| Dilution of sgRNA(s) in injection buffer | Included |
| CRISPR-Cas9 - NHEJ Injection of 150 1-cell embryos (C57BL/6 strain), implantation and biopsies from offspring | 65 000 NOK |
| CRISPR-Cas9 - HDR Injection of 200 1-cell embryos (C57BL/6 strain), implantation and biopsies from offspring | 80 000 NOK |
| Additional rounds of injection | 15 000 NOK/injection day |
| Separation, biopsies | Included |
| Assistance for shipment of mice | Included |
| Health certificate, quarterly health monitoring (SFP status) |
Included |
| Cost for shipment of mice | Payed by customer |
| Cage cost litters from 7 weeks of age | 150 NOK/cage/week |
Provisions: The costs above include housing of offspring until 6 weeks of age. After 6 weeks of age, NTS will charge 150 NOK/cage/week until animals are shipped.
Terms NHJE projects: For projects where NTS has designed and in vitro translated sgRNA(s): NTS guaranties delivery of 4+ genetically altered offspring (out of 25+ offspring) or at least one ‘successful’ founder. A ‘successful founder’ has a genetic alteration(s) resulting in frame shift (or stop codon) in the protein coding sequence.
If NTS delivers no founders that can be considered ‘successful’, the customer will be charged parts of the injection cost fee (3 founders – 90%, 2 founders 80%, 1 founder 60%).
For projects where the customer has designed and delivered sgRNAs, NTS will deliver 25+ offspring and charge full injection costs regardless of the outcome. If no genetically altered founders are found in 25+ screened offspring, NTS will conclude that the sgRNA does not work.
NTS may start over with a new sgRNA if the customer wish so. HDR projects: HDR-editing is more technically advanced and less reliable compared to NHJE-editing. HDR projects requires some protocol adjustments and will required more time to complete. The customer can expect generation of many genetically modified founders due to NHJE repair (various random genetic alterations in the locus without inserting the mutation).
NTS will perform injections until 30+ founders are delivered. With delivery of 1+ correctly modified founder, the customer will be charged full injection costs. Delivery of 3 or less genetically altered founders (e.g. NHJE modifications), but no correctly modified founder, among 30+ offspring will give 40% reduction in the injection fee. NTS may continue injection after 30 live births, but both the customer and NTS have the option to cancel further injections.
The costs of such injections will be 15 000 NOK/day. Restrictions: NTS develops new animal model on a collaborative basis. The partner reimburses running cost and a person named by NTS co-authors the first publication.
Pronuclear injection – Tg construct
| Type of service | Minimum cost (NOK) |
|---|---|
| Digestion, gel purification and dilution of construct | Included |
| Injection of 200 one cell C57BL/6 strain embryos, implantation and biopsies from offspring | 75 000 NOK |
| Additional rounds of injection | 15 000 NOK/injection day |
| Separation, biopsies | Included |
| Assistance for shipment of mice | Included |
| Health certificate, quarterly health monitoring (SFP status) | Included |
| Cost for shipment of mice | Payed by customer |
| Cage cost litters from 7 weeks of age | 160 NOK/cage/week |
Provisions: The cost above includes housing of offspring until 6 weeks of age. After 6 weeks of age, NTS will charge 150 NOK/cage/week until animals are shipped.
NTS offer a guarantee of success within the following terms:
The success rate for Tg-injections are around 10% positive founders. NTS will inject until delivery of 30+ offspring and the customer can expect delivery of 3+ founders (with the construct integrated at different chromosomal locatons).
Projects with delivery of no founders in 30 offspring will be charged 60% of the injection fee (and the project terminated). Delivery of 1 founder will incur 80%, and 2 founders or more 100% of the injection fee. NTS may opt to continue injection after 30 offspring, but both NTS and the customer have the option to cancel further injections.
Restrictions: NTS develops new animal models on a collaborative basis. The partner reimburses running cost and a person named by NTS co-authors the first publication.
ES-cell blastocyst injection
| Type of service | Minimum cost (NOK) |
|---|---|
| Digestion, gel purification and dilution of construct | Included |
| Injection into 100 blastocysts (C57BL/6 strain) | 50 000 NOK |
| Additional rounds of injection | 15 000 NOK/injection day |
| Separation, biopsies | Included |
| Assistance for shipment of mice | Included |
| Health certificate, quarterly health monitoring (SFP status) |
Included |
| Cost for shipment of mice | Payed by customer |
| Cage cost offspring from 7 weeks of age | 150 NOK/cage/week |
Provisions: The cost above includes housing of offspring until 6 weeks of age. After 6 weeks of age, NTS will charge 150 NOK/cage/week until animals are shipped.
Terms: The planned number of injections should normally give rise to 2+ males with more than 30% chimerism. If this is not achieved NTS will perform one more day of injections at no extra cost. Certain conditions apply:
- The customer will be responsible for all ES cell work and must arrange ES cell culturing elsewhere. NTS will be responsible for providing sufficient blastocysts on the planned injection days, perform blastocyst injections, and implant into foster mothers.
- NTS and the customer will agree on a number of injection days, and the customer is responsible for delivering high quality ES cells on the agreed injection days (4 days). Failure to deliver ES cells on planned days will be charged 15 000 NOK or be counted as one “performed injection” day (to cover the costs for hormone injected animals that can not be used another day).
- High pluripotency of the injected ES cells is critical for a successful blastocyst injection. NTS will visually inspect the ES cells and perform a minimal quality check at the day of delivery, but this inspection will be insufficient to determine pluripotency of the ES cells.
- Due to uncertainties with the quality of the delivered ES cells, NTS cannot give any guaranty for success. Customer will be charged the full cost for 4 injections days, regardless of outcome.
Restrictions: NTS develops new animal models on a collaborative basis. The partner reimburses running cost and a person named by NTS co-authors the first publication.
Genotyping
| Type of service | Minimum cost (NOK) |
|---|---|
| Establish a new genotyping protocol | 2500 NOK |
| Validate an existing genotyping protocol (performed once) | 1500 NOK |
| Extraction of DNA from ear or tail biopsy | 40 NOK/sample |
| First PCR reaction | 40 NOK/sample |
| For each extra PCR reaction (2nd reaction on same biopsy) | 20 NOK/sample |
Send an e-mail to transgenic@basalmed.uio.no and we will get back to you with additional information. Fees for the service above is for internal collaborators/customers.
Metabolic Phenotyping
| Type of service | Minimum cost (NOK) |
|---|---|
| Use of Phenomaster (pr. week) | 5000 NOK |
| Technical time to set up/inspect/wash after each experiment | 5000 NOK |
| Measurement of body composition (up to 10 animals) | 1000 NOK |
| Conversion of data into Excel file | free |
| Data analysis | 3000-5000 NOK |
| Cage cost animals | 150 NOK/cage/week |
Provisions:Cage cost for housing of the animals will remain at the customer. For IMB customers, animals can be transferred to the FOTS protocol belonging to NTS during the experiment and transferred back to the original FOTS application belonging to the customer.
For external customers, animals will be transferred to the FOTS protocol belonging to NTS during the experiment and remain on this FOTS for the time agreed upon with the customer.
Restrictions: NTS performs metabolic phenotyping on a collaborative basis. The partner reimburses running cost and a person named by NTS co-authors the first publication.
Mice strains
| Type of service | Minimum cost (NOK) |
|---|---|
| Cre recombinase mice (each) | 2000 NOK* |
| Flp recombinase mice (each) | 2000 NOK* |
| C57BL6/N (each) | 600 NOK** |
Restrictions: *The listed costs for Cre and Flp mice are for living mice. If the model is not in breeding, the cost for these models will equal to the fee for embryo recovery.
**NTS can order C57BL6/N for customers together with our other orders. Contact NTS if you want to order a small number of mice (e.g. for back crossing) and is interested in sharing transport costs.