Muhammad Asaduzzaman

• Khai, Tual Sawn; Ahmed, Minhaz Farid & Asaduzzaman, Muhammad (2024). Examining the vulnerability of persons with disabilities in Myanmar pre and post-COVID-19 and military coup. Disability & Society. ISSN 0968-7599. doi: 10.1080/09687599.2024.2360433.
• Rose, Elizabeth S.; Bello-Manga, Halima; Boafor, Theodore & Asaduzzaman, Muhammad (2023). International collaborative research, systems leadership and education: reflections from academic biomedical researchers in Africa. Frontiers in Education. ISSN 2504-284X. 8. doi: 10.3389/feduc.2023.1217066.
• Asaduzzaman, Muhammad; Mekonnen, Zeleke; Rødland, Ernst Kristian; Sahay, Sundeep; Winkler, Andrea Sylvia & Gradmann, Christoph (2023). District health information system (DHIS2) as integrated antimicrobial resistance surveillance platform: An exploratory qualitative investigation of the one health stakeholders’ viewpoints in Ethiopia. International Journal of Medical Informatics. ISSN 1386-5056. 181. doi: 10.1016/j.ijmedinf.2023.105268. Fulltekst i vitenarkiv
• Ziveri, Davide & Asaduzzaman, Muhammad (2023). International Non-Governmental Organizations (INGOs) in Humanitarian Field: why and how to engage with Planetary Health? Qeios. ISSN 2632-3834. doi: 10.32388/1BHA16. Fulltekst i vitenarkiv
• Habiba, Um E; Khan, Amjad; Mmbaga, Elia; Green, Ivan Robert & Asaduzzaman, Muhammad (2023). Use of antibiotics in poultry and poultry farmers- a cross-sectional survey in Pakistan. Frontiers in Public Health. ISSN 2296-2565. 11. doi: 10.3389/fpubh.2023.1154668. Fulltekst i vitenarkiv
• Khai, Tual Sawn & Asaduzzaman, Muhammad (2023). Preventive knowledge, attitude, and vaccination challenges for COVID-19 among Myanmar refugees and irregular migrants in Malaysia. Vaccine: X. 15. doi: 10.1016/j.jvacx.2023.100360. Fulltekst i vitenarkiv
• Wunrow, Han Yong; Bender, Rose G; Vongpradith, Avina ; Sirota, Sarah Brooke ; Swetschinski, Lucien R  & Novotney, Amanda  [Vis alle 51 forfattere av denne artikkelen] (2023). Global, regional, and national burden of meningitis and its aetiologies, 1990–2019: a systematic analysis for the Global Burden of Disease Study 2019. Lancet Neurology. ISSN 1474-4422. 22(8), s. 685–711. doi: 10.1016/S1474-4422(23)00195-3. Fulltekst i vitenarkiv
• Oladosu, Ayomide Oluwaseyi; Khai, Tual Sawn & Asaduzzaman, Muhammad (2023). Factors affecting access to healthcare for young people in the informal sector in developing countries: a systematic review. Frontiers in Public Health. ISSN 2296-2565. 11. doi: 10.3389/fpubh.2023.1168577.
• Devnath, Popy; Karah, Nabil; Graham, Jay P.; Rose, Elizabeth S. & Asaduzzaman, Muhammad (2023). Evidence of Antimicrobial Resistance in Bats and Its Planetary Health Impact for Surveillance of Zoonotic Spillover Events: A Scoping Review. International Journal of Environmental Research and Public Health (IJERPH). ISSN 1661-7827. 20(1). doi: 10.3390/ijerph20010243. Fulltekst i vitenarkiv Vis sammendrag

  As a result of the COVID-19 pandemic, as well as other outbreaks, such as SARS and Ebola, bats are recognized as a critical species for mediating zoonotic infectious disease spillover events. While there is a growing concern of increased antimicrobial resistance (AMR) globally during this pandemic, knowledge of AMR circulating between bats and humans is limited. In this paper, we have reviewed the evidence of AMR in bats and discussed the planetary health aspect of AMR to elucidate how this is associated with the emergence, spread, and persistence of AMR at the human–animal interface. The presence of clinically significant resistant bacteria in bats and wildlife has important implications for zoonotic pandemic surveillance, disease transmission, and treatment modalities. We searched MEDLINE through PubMed and Google Scholar to retrieve relevant studies (n = 38) that provided data on resistant bacteria in bats prior to 30 September 2022. There is substantial variability in the results from studies measuring the prevalence of AMR based on geographic location, bat types, and time. We found all major groups of Gram-positive and Gram-negative bacteria in bats, which are resistant to commonly used antibiotics. The most alarming issue is that recent studies have increasingly identified Methicillin Resistant Staphylococcus aureus (MRSA), ESBL producing, and Colistin resistant Enterobacterales in samples from bats. This evidence of superbugs abundant in both humans and wild mammals, such as bats, could facilitate a greater understanding of which specific pathways of exposure should be targeted. We believe that these data will also facilitate future pandemic preparedness as well as global AMR containment during pandemic events and beyond.

• Naghavi, Mohsen; Ikuta, Kevin S.; Swetschinski, Lucien R.; Augilar, Gisela Robles; Sharara, Fablina & Mestrovic, Tomislav [Vis alle 9 forfattere av denne artikkelen] (2022). Global mortality associated with 33 bacterial pathogens in 2019: a systematic analysis for the Global Burden of Disease Study 2019. The Lancet. ISSN 0140-6736. 400(10369), s. 2221–2248. doi: 10.1016/S0140-6736(22)02185-7. Fulltekst i vitenarkiv
• Amin, Mohammed Badrul; Talukdar, Prabhat Kumar; Asaduzzaman, Muhammad; Roy, Subarna; Flatgard, Brandon M. & Islam, Md Rayhanul [Vis alle 13 forfattere av denne artikkelen] (2022). Effects of chronic exposure to arsenic on the fecal carriage of antibiotic-resistant Escherichia coli among people in rural Bangladesh. PLoS Pathogens. ISSN 1553-7366. 18(12). doi: 10.1371/journal.ppat.1010952. Fulltekst i vitenarkiv Vis sammendrag

  Antibiotic resistance is a leading cause of hospitalization and death worldwide. Heavy met als such as arsenic have been shown to drive co-selection of antibiotic resistance, suggest ing arsenic-contaminated drinking water is a risk factor for antibiotic resistance carriage.This study aimed to determine the prevalence and abundance of antibiotic-resistant Escher ichia coli (AR-Ec) among people and drinking water in high (Hajiganj, >100 μg/L) and low arsenic-contaminated (Matlab, <20 μg/L) areas in Bangladesh. Drinking water and stool from mothers and their children (<1 year) were collected from 50 households per area. AR Ec was detected via selective culture plating and isolates were tested for antibiotic resis tance, arsenic resistance, and diarrheagenic genes by PCR. Whole-genome sequencing (WGS) analysis was done for 30 E. coli isolates from 10 households. Prevalence of AR-Ec was significantly higher in water in Hajiganj (48%) compared to water in Matlab (22%, p<0.05) and among children in Hajiganj (94%) compared to children in Matlab (76%, p<0.05), but not among mothers. A significantly higher proportion of E. coli isolates from Haji ganj were multidrug-resistant (83%) compared to isolates from Matlab (71%, p <0.05). Co resistance to arsenic and multiple antibiotics (MAR index >0.2) was observed in a higher proportion of water (78%) and child stool (100%) isolates in Hajiganj than in water (57%) and children (89%) in Matlab (p <0.05). The odds of arsenic-resistant bacteria being resis tant to third-generation cephalosporin antibiotics were higher compared to arsenic-sensitive bacteria (odds ratios, OR 1.2–7.0, p <0.01). WGS-based phylogenetic analysis of E. coli iso lates did not reveal any clustering based on arsenic exposure and no significant difference in resistome was found among the isolates between the two areas. The positive association detected between arsenic exposure and antibiotic resistance carriage among children in arsenic-affected areas in Bangladesh is an important public health concern that warrants redoubling efforts to reduce arsenic exposure.

• Khai, Tual Sawn & Asaduzzaman, Muhammad (2022). ‘I Doubt Myself and Am Losing Everything I Have since COVID Came’—A Case Study of Mental Health and Coping Strategies among Undocumented Myanmar Migrant Workers in Thailand. International Journal of Environmental Research and Public Health (IJERPH). ISSN 1661-7827. 19(22). doi: 10.3390/ijerph192215022. Fulltekst i vitenarkiv Vis sammendrag

  Migrant populations have always been vulnerable to a high burden of social exclusion, mental disorders, physical illnesses, and economic crises. The current COVID-19 pandemic has further created a frantic plight among them, particularly for undocumented migrant workers in the global south. We have conducted a mixed method study among the undocumented Myanmar migrant workers (UMMWs) in Thailand to explore how the COVID-19 disruption has impacted their mental health and what coping strategies they have adopted. Following the onset of COVID-19 and the recent coup d’état in Myanmar, our current study is the first attempt to understand the mental health status and predicament of this neglected migrant group. A total of 398 UMMWs were included in the online survey, of which 23 participated in qualitative interviews. The major mental health issues reported by the study participants were depression, generalized anxiety disorder, frustration, stress, and panic disorders, while loss of employment, worries about the pandemic, social stigma, lack of access to healthcare, lockdown, and fear of detention were the predominant contributing factors. In response, we identified two key coping mechanisms: coping at a personal level (listening to music, playing online game, praying, and self-motivation) and coping at a social level (chatting with family and friends and visiting religious institutions). These findings point to the importance of policy and intervention programs aimed at upholding mental health at such humanitarian conditions. Sustainable institutional mental health care support and social integration for the migrant workers, irrespective of their legal status, should be ensured.

• Islam, Mohammad Aminul; Islam, Md Rayhanul; Khan, Rizwana; Badrul Amin, Mohammed; Rahman, Mahdia & Hossain, Muhammed Iqbal [Vis alle 9 forfattere av denne artikkelen] (2022). Prevalence, etiology and antibiotic resistance patterns of community-Acquired urinary tract infections in Dhaka, Bangladesh. PLOS ONE. ISSN 1932-6203. 17(9). doi: 10.1371/journal.pone.0274423. Fulltekst i vitenarkiv
• Asaduzzaman, Muhammad; Ara, Rifat; Afrin, Sadia; Meiring, James E. & Saif-Ur-Rahman, K. M. (2022). Planetary Health Education and Capacity Building for Healthcare Professionals in a Global Context: Current Opportunities, Gaps and Future Directions. International Journal of Environmental Research and Public Health (IJERPH). ISSN 1661-7827. 19(18). doi: 10.3390/ijerph191811786. Fulltekst i vitenarkiv Vis sammendrag

  The emerging concept of planetary health needs to be discussed in a more organized and sustainable way within the global public health and healthcare disciplines. Therefore, planetary health should be considered a cardinal component of the global academic framework for healthcare professionals. The availability of related curricula and courses is crucial to equip health professionals in this relatively new discipline of planetary health. In this review article, we aimed to explore published articles and online databases of courses to summarize the available planetary health education opportunities and discussions for health professionals, to identify the gaps in resource allocation and to suggest future recommendations. We observed a visible resource inequity in the global south with the lack of a universal planetary health module for healthcare professionals. Additionally, there is minimal inclusion of allied health disciplines in this learning process. We therefore recommend a dedicated network of motivated healthcare professionals and regional hubs with an agenda to ensure a comprehensive, uniform, and inclusive planetary health education curriculum and practice.

• Clarsen, Benjamin Matthew; Nylenna, Magne; Klitkou, Søren Toksvig; Vollset, Stein Emil; Baravelli, Carl Michael & Bølling, Anette Kocbach [Vis alle 31 forfattere av denne artikkelen] (2022). Changes in life expectancy and disease burden in Norway, 1990–2019: an analysis of the Global Burden of Disease Study 2019. The Lancet Public Health. ISSN 2468-2667. 7(7), s. e593–e605. doi: 10.1016/S2468-2667(22)00092-5. Fulltekst i vitenarkiv Vis sammendrag

  Background: Geographical differences in health outcomes are reported in many countries. Norway has led an active policy aiming for regional balance since the 1970s. Using data from the Global Burden of Disease Study (GBD) 2019, we examined regional differences in development and current state of health across Norwegian counties. Methods: Data for life expectancy, healthy life expectancy (HALE), years of life lost (YLLs), years lived with disability (YLDs), and disability-adjusted life-years (DALYs) in Norway and its 11 counties from 1990 to 2019 were extracted from GBD 2019. County-specific contributors to changes in life expectancy were compared. Inequality in disease burden was examined by use of the Gini coefficient. Findings: Life expectancy and HALE improved in all Norwegian counties from 1990 to 2019. Improvements in life expectancy and HALE were greatest in the two counties with the lowest values in 1990: Oslo, in which life expectancy and HALE increased from 71·9 years (95% uncertainty interval 71·4-72·4) and 63·0 years (60·5-65·4) in 1990 to 81·3 years (80·0-82·7) and 70·6 years (67·4-73·6) in 2019, respectively; and Troms og Finnmark, in which life expectancy and HALE increased from 71·9 years (71·5-72·4) and 63·5 years (60·9-65·6) in 1990 to 80·3 years (79·4-81·2) and 70·0 years (66·8-72·2) in 2019, respectively. Increased life expectancy was mainly due to reductions in cardiovascular disease, neoplasms, and respiratory infections. No significant differences between the national YLD or DALY rates and the corresponding age-standardised rates were reported in any of the counties in 2019; however, Troms og Finnmark had a higher age-standardised YLL rate than the national rate (8394 per 100 000 [95% UI 7801-8944] vs 7536 per 100 000 [7391-7691]). Low inequality between counties was shown for life expectancy, HALE, all level-1 causes of DALYs, and exposure to level-1 risk factors. Interpretation: Over the past 30 years, Norway has reduced inequality in disease burden between counties. However, inequalities still exist at a within-county level and along other sociodemographic gradients. Because of insufficient Norwegian primary data, there remains substantial uncertainty associated with regional estimates for non-fatal disease burden and exposure to risk factors.

• Asaduzzaman, Muhammad; Rousham, Emily Kate; Unicomb, Leanne; Islam, Md. Rayhanul; Amin, Mohammed Badrul & Rahman, Mahdia [Vis alle 10 forfattere av denne artikkelen] (2022). Spatiotemporal distribution of antimicrobial resistant organisms in different water environments in urban and rural settings of Bangladesh. Science of the Total Environment. ISSN 0048-9697. doi: 10.1016/j.scitotenv.2022.154890. Fulltekst i vitenarkiv Vis sammendrag

  The spatial distribution of clinically important antibiotic resistant bacteria (ARB) and associated genes is important to identify environmental distribution of contamination and ‘hotspots’ of antimicrobial resistance (AMR). We conducted an integrated survey of AMR in drinking water, wastewater and surface water (rivers and ponds) in three settings in Bangladesh: rural households, rural poultry farms, and urban food markets. Spatial mapping was conducted via geographic information system (GIS) using ArcGIS software. Samples (n = 397) were analyzed for the presence of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-Ec), carbapenem-resistant E. coli (CR-Ec) and resistance genes (blaCTX-M-1, blaNDM-1). In rural households, 5% of drinking water supply samples tested positive for ESBL-Ec, and a high proportion of wastewater, pond and river water samples were positive for ESBL-Ec (90%, 76%, and 85%, respectively). In poultry farms, 10% of drinking water samples tested positive for ESBL-Ec compared to a high prevalence in wastewater, pond and river water (90%, 68%, and 85%, respectively). CR-Ec prevalence in household wastewater and pond water was relatively low (8% and 5%, respectively) compared to river water (33%). In urban areas, 38% of drinking water samples and 98% of wastewater samples from markets tested positive for ESBL-Ec while 30% of wastewater samples tested positive for CR-Ec. Wastewaters had the highest concentrations of ESBL-Ec, CR-Ec, blaCTXM-1 and blaNDM-1 and these were significantly higher in urban compared to rural samples (p < 0.05). ESBL-Ec is ubiquitous in drinking water, wastewater and surface water bodies in both rural and urban areas of Bangladesh. CR-Ec is less widespread but found at a high prevalence in wastewater discharged from urban food markets and in rural river samples. Surveillance and monitoring of antibiotic resistant organisms and genes in waterbodies is an important first step in addressing environmental dimensions of AMR.

• Boriani, Elena; Aragrande, Maurizio; Canali, Massimo; Balzan, Mario V. & Asaduzzaman, Muhammad (2021). Pragmatic use of Planetary Health and nature based solutions for future pandemics using COVID-19 case scenario. Frontiers in Public Health. ISSN 2296-2565. doi: 10.3389/fpubh.2021.620120.
• Sarker, Tanwne; Sarkar, Apurbo; Ghulam Rabbany, Md.; Barmon, Milon; Roy, Rana & Rahman, Md. Ashfikur [Vis alle 9 forfattere av denne artikkelen] (2021). Evaluation of preventive, supportive and awareness building measures among international students in China in response to COVID-19: a structural equation modeling approach. Global health research and policy. ISSN 2397-0642. 6. doi: 10.1186/s41256-021-00192-5. Fulltekst i vitenarkiv Vis sammendrag

  Background Education institutions promptly implemented a set of steps to prevent the spread of COVID-19 among international Chinese students, such as restrictive physical exercise, mask wear, daily health reporting, etc. Success of such behavioral change campaigns largely depends on awareness building, satisfaction and trust on the authorities. The purpose of this current study is to assess the preventive, supportive and awareness-building steps taken during the COVID-19 pandemic for international students in China, that will be useful for planning such a behavioral change campaign in the potential pandemic situation in other parts of the world. Methods We conducted an online-based e-questionnaire survey among 467 international students in China through WeChat. The data collection duration was from February 20, 2020 to March 10, 2020 and we focused on their level of awareness, satisfaction, and trust in authorities regarding pandemic measures. Simple bivariate statistics was used to describe the background characteristics of the respondents along with adoption of the partial least squares-structural equation modeling (PLS-SEM) as the final model to demonstrate the relationship between the variables. Results In our study, the leading group of the respondents were within 31 to 35 years’ age group (39.82%), male (61.88%), living single (58.24%) and doctoral level students (39.8%). The preventive and supportive measures taken by students and/or provided by the respective institution or authorities were positively related to students’ satisfaction and had an acceptable strength (β = 0.611, t = 9.679, p < 0.001). The trust gained in authorities also showed an acceptable strength (β = 0.381, t = 5.653, p < 0.001) with a positive direction. Again, the personnel awareness building related to both students’ satisfaction (β = 0.295, t = 2.719, p < 0.001) and trust gain (β = 0.131, t = 1.986, p < 0.05) in authorities had a positive and acceptable intensity. Therefore, our study clearly demonstrates the great impact of preventive and supportive measures in the development of students’ satisfaction (R2 = 0.507 indicating moderate relationship). The satisfied students possessed a strong influence which eventually helped in building sufficient trust on their institutions (R2 = 0.797 indicating above substantial relationship). Conclusions The worldwide student group is one of the most affected and vulnerable communities in this situation. So, there is a profound ground of research on how different states or authorities handle such situation. In this study, we have depicted the types and magnitude of care taken by Chinese government and educational institutions towards international students to relieve the panic of pandemic situation. Further research and such initiatives should be taken in to consideration for future emerging conditions.

• Rousham, Emily K; Asaduzzaman, Muhammad; Mozmader, T.I.M. Amin Uddin; Amin, Mohammed Badrul; Rahman, Mahdia & Hossain, Muhammed Iqbal [Vis alle 10 forfattere av denne artikkelen] (2021). Human Colonization with Extended-Spectrum Beta-Lactamase-Producing E. coli in Relation to Animal and Environmental Exposures in Bangladesh: An Observational One Health Study. Environmental Health Perspectives. ISSN 0091-6765. 129(3). doi: 10.1289/EHP7670. Fulltekst i vitenarkiv Vis sammendrag

  Background: Human exposure to intensively farmed livestock is a potential risk for transmission of antibiotic-resistant bacteria (ARB) but few studies have assessed the relative role of animal vs. environmental sources of ARB in low-resource community settings. Objectives: We conducted an observational study to compare ARB colonization and antibiotic-resistant gene prevalence and abundance in humans with high or low exposure to poultry in rural households, commercial poultry farms, and urban markets in Bangladesh. Methods: Extended-spectrum β-lactamase (ESBL)-producing and carbapenem-resistant E. coli were quantified in feces from adults with high or low poultry exposure (n=100, respectively), poultry (n=200), drinking water (n=120), and wastewater (n=120) from 40 rural households, 40 poultry farms, and 40 urban markets. Results: ESBL-producing E. coli (ESBL-EC) prevalence was 67.5% (95% CI: 61.0, 74.0) in samples from adults, 68.0% (95% CI: 61.5, 74.5) in samples from poultry, and 92.5% (95% CI: 87.7, 97.3) in wastewater samples. Carbapenem-resistant E. coli prevalence was high in market wastewaters [30% (95% CI: 15.0, 45.0)] but low in humans (1%) and poultry (1%). Human, poultry, and wastewater isolates shared common resistance genes: blaCTX-M-1, qnr, and blaTEM. Human colonization was not significantly associated with exposure to poultry or setting (rural, farm, or market). Ninety-five percent of commercial poultry farms routinely administered antibiotics. Susceptibility tests were significantly different in household vs. farm and market poultry isolates for four of seven antibiotic classes. In human isolates, there were no differences except aminoglycoside resistance (16.4% high vs. 4.4% low exposure, p=0.02). Urban market wastewaters and poultry samples had significantly higher concentrations of ESBL-EC (p<0.001) and blaCTX-M-1 (p<0.001) compared with samples from farms and rural households. Discussion: ESBL-EC colonization was high in humans but not significantly associated with exposure to poultry. Bidirectional transmission of antibiotic resistance is likely between humans, poultry, and the environment in these community settings, underlining the importance of One Health mitigation strategies.

• Al masud, Abdullah; Rousham, Emily Kate; Islam, Mohammad Aminul; Alam, Mahbub-Ul; Rahman, Mahbubur & Al Mamun, Abdullah [Vis alle 9 forfattere av denne artikkelen] (2020). Drivers of Antibiotic Use in Poultry Production in Bangladesh: Dependencies and Dynamics of a Patron-Client Relationship. Frontiers in Veterinary Science. ISSN 2297-1769. doi: 10.3389/fvets.2020.00078. Fulltekst i vitenarkiv Vis sammendrag

  Background: There is increasing concern around the use of antibiotics in animal food production and the risk of transmission of antimicrobial resistance within the food chain. In many low and middle-income countries, including Bangladesh, the commercial poultry sector comprises small-scale producers who are dependent on credit from poultry dealers to buy day-old chicks and poultry feed. The same dealers also supply and promote antibiotics. The credit system is reliant upon informal relationships among multiple actors as part of social capital. This paper aims to describe dependencies and relationships between different actors within unregulated broiler poultry production systems to understand the social and contextual determinants of antibiotic use in low-resource settings. Methods: We used a cross-sectional qualitative design including in-depth interviews among purposefully selected commercial poultry farmers (n = 10), poultry dealers (n = 5), sales representatives of livestock pharmaceutical companies (n = 3) and the local government livestock officer as a key-informant (n = 1). We describe the food production cycle and practices relating to credit purchases and sales using social capital theory. Findings: Poultry dealers provide credit and information for small-scale poultry farmers to initiate and operate their business. In return for credit, farmers are obliged to buy poultry feed and medicine from their dealer and sell their market-ready poultry to that same dealer. All farms applied multiple antibiotics to poultry throughout the production cycle, including banned antibiotics such as colistin sulfate. The relationship between dealers and poultry farmers is reciprocal but mostly regulated by the dealers. Dealers were the main influencers of decision-making by farmers, particularly around antibiotic use as an integral part of the production cycle risk management. Our findings suggest that strategies to improve antibiotic stewardship and responsible use should exploit the patron-client relationship which provides the social and information network for small-scale farmers. Conclusion: Social capital theory can be applied to the patron-client relationship observed among poultry farmers and dealers in Bangladesh to identify influences on decision making and antibiotic use. Within unregulated food production systems, strategies to promote the prudent use of antibiotics should target commercial feed producers and livestock pharmaceutical manufacturers as a first step in developing a sustainable poultry value chain.

• Islam, Mohammad Aminul; Amin, Mohammed Badrul; Roy, Subarna; Asaduzzaman, Muhammad; Islam, Md.Rayhanul & Navab-Daneshmand, Tala [Vis alle 10 forfattere av denne artikkelen] (2019). Fecal Colonization With Multidrug-Resistant E. coli Among Healthy Infants in Rural Bangladesh. Frontiers in Microbiology. ISSN 1664-302X. doi: 10.3389/fmicb.2019.00640. Vis sammendrag

  Third generation cephalosporins (3GC) are one of the main choices for treatment of infections caused by multidrug-resistant (MDR) Gram-negative bacteria. Due to their overuse, an increasing trend of resistance to 3GC has been observed in developing countries. Here, we describe fecal colonization of 3GC-resistant (3GCr) Escherichia coli in healthy infants (1-12 months old) living in rural areas of Bangladesh. We found that stool samples of 82% of infants (n = 100) were positive for 3GCr E. coli with a mean ± standard deviation of 6.21 ± 1.32 log10 CFU/g wet weight of stool. 3GCr E. coli encompasses an average one third (33%) of the total E. coli of stool. Almost 77% (n = 63) of these 3GCr E. coli were MDR (or resistant to ≥3 classes of antibiotics). Around 90% (n = 74) of 3GCr E. coli were extended spectrum beta-lactamase (ESBL)-producing in which bla CTX-M-group-1 was the predominant (96%, n = 71) ESBL-gene followed by bla TEM (41%, n = 30) and bla OXA-1 (11%, n = 8). A significant proportion (26.5%, n = 22) of 3GCr E. coli was pathogenic, comprising two types, enteroaggregative (EAEC, n = 19) and enteropathogenic (EPEC, n = 3). Colonization of 3GCr E. coli in infant guts was not associated with demographic characteristics such as age, sex, mode of delivery, maternal and infant antibiotic use, disease morbidity, and feeding practices. The high rate of colonization of 3GCr E. coli in infants' guts is a serious public health concern which needs immediate attention and warrants further studies to explore the cause. KEYWORDS: E. coli; ESBL; colonization; multidrug-resistant; third generation cephalosporins

• Alam, Mahbub-Ul; Rahman, Mahbubur; Masud, Abdullah-Al-; Islam, Mohammad Aminul; Asaduzzaman, Muhammad & Sarker, Supta [Vis alle 8 forfattere av denne artikkelen] (2019). Human exposure to antimicrobial resistance from poultry production: Assessing hygiene and waste-disposal practices in Bangladesh. International Journal of Hygiene and Environmental Health. ISSN 1438-4639. 222(8), s. 1068–1076. doi: 10.1016/j.ijheh.2019.07.007. Vis sammendrag

  The unregulated use of antibiotics is linked with intensive poultry farming in developing economies. In low-and middle-income countries, the dissemination of antimicrobial resistance (AMR) has also been attributed to contamination, poor public health infrastructure and inadequate waste disposal practices. There are limited data on hygiene and waste disposal practices in small-scale commercial and household poultry farming and market sales in Bangladesh. Our objective was to explore human exposures, hygiene and waste disposal practices in poultry raising and processing to identify probable pathways for transmission of AMR bacteria.

• Asaduzzaman, Muhammad; Muhammed, Iqbal Hossain; Saha, Sumita Rani; Islam, Rayhanu; Ahmed, Niyaz & Islam, Mohammad Aminul (2019). Quantification of Airborne Resistant Organisms With Temporal and Spatial Diversity in Bangladesh: Protocol for a Cross-Sectional Study. JMIR Research Protocols. ISSN 1929-0748. 8(12). doi: 10.2196/14574. Fulltekst i vitenarkiv Vis sammendrag

  Background Antimicrobial resistance is a widespread, alarming issue in global health and a significant contributor to human death and illness, especially in low and middle-income countries like Bangladesh. Despite extensive work conducted in environmental settings, there is a scarcity of knowledge about the presence of resistant organisms in the air. Objective The objective of this protocol is to quantify and characterize the airborne resistomes in Bangladesh, which will be a guide to identify high-risk environments for multidrug-resistant pathogens with their spatiotemporal diversity. Methods This is a cross-sectional study with an environmental, systematic, and grid sampling strategy focused on collecting air samples from different outdoor environments during the dry and wet seasons. The four environmental compartments are the frequent human exposure sites in both urban and rural settings: urban residential areas (n=20), live bird markets (n=20), rural households (n=20), and poultry farms (n=20). We obtained air samples from 80 locations in two seasons by using an active microbial air sampler. From each location, five air samples were collected in different media to yield the total bacterial count of 3rd generation cephalosporin (3GC) resistant Enterobacteriaceae, carbapenem-resistant Enterobacteriaceae, vancomycin-resistant Enterococci and methicillin-resistant Staphylococcus aureus. Results The study started in January 2018, and the collection of air samples was completed in November 2018. We have received 800 air samples from 80 study locations in both dry and wet seasons. Currently, the laboratory analysis is ongoing, and we expect to receive the preliminary results by October 2019. We will publish the complete result as soon as we clean and analyze the data and draft the manuscript. Conclusions The existence of resistant bacteria in the air like those producing extended-spectrum beta-lactamases, carbapenem-resistant Enterobacteriaceae, vancomycin-resistant Enterococci, and methicillin-resistant Staphylococcus aureus will justify our hypothesis that the outdoor environment (air) in Bangladesh acts as a reservoir for bacteria that carry genes conferring resistance to antibiotics. To our knowledge, this is the first study to explore the presence of superbugs in the air in commonly exposed areas in Bangladesh. International Registered Report Identifier (IRRID) DERR1-10.2196/14574 Keywords: antimicrobial resistance, airborne resistomes, air quality, global health, planetary health, environmental risk assessment

• Islam, Mohammad Zahirul; Rutherford, Shannon; Phung, Dung; Uzzaman, Md. Nazim; Baum, Scott & Huda, M. Mamun [Vis alle 9 forfattere av denne artikkelen] (2018). Correlates of Climate Variability and Dengue Fever in Two Metropolitan Cities in Bangladesh . Cureus. ISSN 2168-8184. 10(10). doi: 10.7759/cureus.3398. Fulltekst i vitenarkiv Vis sammendrag

  Dengue fever is a major public health concern in Bangladesh with increased incidence during monsoon. We aimed to assess the correlation of temperature, humidity, and rainfall on dengue fever in two dengue endemic cities in Bangladesh. It was a time series analysis of climate factors and dengue occurrence data in Dhaka and Chittagong cities from 1 January 2000 to 31 December 2009. Daily mean temperature, rainfall, and humidity data were obtained from the Bangladesh meteorological department and daily dengue cases data were obtained from the directorate general of health services (DGHS) of Bangladesh. The mean dengue incidence was 31.62 (SD 28.7) per 100,000 in Dhaka whereas it was 5.76 (SD 11.7) per 100,000 population in Chittagong. The incidence of dengue cases was found significantly associated with the monthly mean temperature, total rainfall, and mean humidity in Dhaka, though in Chittagong, the significantly associated factors​ ​​​​​​were monthly total rainfall and mean humidity. The autoregressive integrated moving average (ARIMA) model identified monthly mean humidity and total rainfall as the most significant contributing factors for dengue cases in Dhaka and Chittagong, respectively. Our study reinforces the relationship of climate parameters with dengue fever, which will support policy-makers in developing a climate-based early warning system for dengue in Bangladesh.

• Ali, Nausad; Sultana, Marufa; Sheikh, Nurnabi; Akram, Raisul; Mahumud, Rashidul Alam & Asaduzzaman, Muhammad [Vis alle 7 forfattere av denne artikkelen] (2018). Predictors of Optimal Antenatal Care Service Utilization Among Adolescents and Adult Women in Bangladesh. Health Services Research and Managerial Epidemiology. ISSN 2333-3928. 5(1). doi: 10.1177/2333392818781729. Fulltekst i vitenarkiv Vis sammendrag

  Introduction: Utilization of recommended antenatal care (ANC) throughout the pregnancy period is a proven healthy behavior in reducing maternal mortalities and morbidities. The objective of this study is to identify the demand side factors that are associated with the recommended utilization of ANC services among adolescents and adult women in Bangladesh. Method: This study utilized cross-sectional data from latest Bangladesh Demographic and Health Survey 2014. Data of a total of 4626 adolescents and adult women were analyzed. Bivariate and multivariate analyses were performed for identifying the significant determining factors associated with the ANC services utilization. Results: Approximately, 32% adult and 30% adolescent women utilized the recommended ANC care. The higher educated adolescents and adult women were 8.08 times (P

• Sheikh, Nurnabi; Sultana, Marufa; Ali, Nausad; Akram, Raisul; Mahumud, Rashidul Alam & Asaduzzaman, Muhammad [Vis alle 7 forfattere av denne artikkelen] (2018). Coverage, Timelines, and Determinants of Incomplete Immunization in Bangladesh. Tropical Medicine and Infectious Disease. ISSN 2414-6366. 3(72). doi: 10.3390/tropicalmed3030072. Fulltekst i vitenarkiv Vis sammendrag

  BACKGROUND The objective of this study was to estimate the extent of timely immunization coverage and to investigate the determinants of incomplete and untimely vaccination. METHODS: The study used data from the latest Bangladesh Demographic Health Survey (BDHS) 2014. A total sample of 1631 children aged 12⁻23 months who had an Expanded Program on Immunization (EPI) card and immunization history were analyzed. RESULTS: The proportions of children who received timely vaccinations were 24% for BCG, 46% for pentavalent 3, and 53% for measles, whereas 76%, 51%, and 36% children failed to receive the BCG, pentavalent 3, and measles vaccines, respectively, in a timely manner. CONCLUSIONS: The study identified some key determinants of untimely and incomplete childhood vaccinations in the context of Bangladesh. The findings will contribute to the improvement of age-specific vaccination and support policy makers in taking the necessary control strategies with respect to delayed and early vaccination in Bangladesh.

• Qadri, Firdausi; Ali, Mohammad; Lynch, Julia; Chowdhury, Fahima; Khan, Ashraful Islam & Wierzba, Thomas F [Vis alle 25 forfattere av denne artikkelen] (2018). Efficacy of a single-dose regimen of inactivated whole-cell oral cholera vaccine: results from 2 years of follow-up of a randomised trial. The Lancet Infectious Diseases. ISSN 1473-3099. doi: 10.1016/S1473-3099(18)30108-7. Vis sammendrag

  Background A single-dose regimen of inactivated whole-cell oral cholera vaccine (OCV) is attractive because it reduces logistical challenges for vaccination and could enable more people to be vaccinated. Previously, we reported the efficacy of a single dose of an OCV vaccine during the 6 months following dosing. Herein, we report the results of 2 years of follow-up. Methods In this placebo-controlled, double-blind trial done in Dhaka, Bangladesh, individuals aged 1 year or older with no history of receipt of OCV were randomly assigned to receive a single dose of inactivated OCV or oral placebo. The primary endpoint was a confirmed episode of non-bloody diarrhoea for which the onset was at least 7 days after dosing and a faecal culture was positive for Vibrio cholerae O1 or O139. Passive surveillance for diarrhoea was done in 13 hospitals or major clinics located in or near the study area for 2 years after the last administered dose. We assessed the protective efficacy of the OCV against culture-confirmed cholera occurring 7–730 days after dosing with both crude and multivariable per-protocol analyses. This trial is registered at ClinicalTrials.gov, number NCT02027207. Findings Between Jan 10, 2014, and Feb 4, 2014, 205 513 people were randomly assigned to receive either vaccine or placebo, of whom 204 700 (102 552 vaccine recipients and 102 148 placebo recipients) were included in the per-protocol analysis. 287 first episodes of cholera (109 among vaccine recipients and 178 among placebo recipients) were detected during the 2-year follow-up; 138 of these episodes (46 in vaccine recipients and 92 in placebo recipients) were associated with severe dehydration. The overall incidence rates of initial cholera episodes were 0·22 (95% CI 0·18 to 0·27) per 100 000 person-days in vaccine recipients versus 0·36 (0·31 to 0·42) per 100 000 person-days in placebo recipients (adjusted protective efficacy 39%, 95% CI 23 to 52). The overall incidence of severe cholera was 0·09 (0·07 to 0·12) per 100 000 person-days versus 0·19 (0·15 to 0·23; adjusted protective efficacy 50%, 29 to 65). Vaccine protective efficacy was 52% (8 to 75) against all cholera episodes and 71% (27 to 88) against severe cholera episodes in participants aged 5 years to younger than 15 years. For participants aged 15 years or older, vaccine protective efficacy was 59% (42 to 71) against all cholera episodes and 59% (35 to 74) against severe cholera. The protection in the older age groups was sustained throughout the 2-year follow-up. In participants younger than 5 years, the vaccine did not show protection against either all cholera episodes (protective efficacy −13%, −68 to 25) or severe cholera episodes (−44%, −220 to 35). Interpretation A single dose of the inactivated whole-cell OCV offered protection to older children and adults that was sustained for at least 2 years. The absence of protection of young children might reflect a lesser degree of pre-existing natural immunity in this age group. Funding Bill & Melinda Gates Foundation to the International Vaccine Institute.

• Asaduzzaman, Muhammad; Chowdhury, Shahanaz; Shahed, Jahid Hossen; Kafi, Mohammad Abdullah Heel; Uzzaman, Md. Nazim & Flowra, Mahfuza Talukder [Vis alle 7 forfattere av denne artikkelen] (2018). Prevalence of Type 2 Diabetes Mellitus Among Urban Bihari Communities in Dhaka, Bangladesh: A Cross-sectional Study in a Minor Ethnic Group . Cureus. ISSN 2168-8184. doi: 10.7759/cureus.2116. Fulltekst i vitenarkiv Vis sammendrag

  Introduction The prevalence, disease progression, and treatment outcomes for patients with type 2 diabetes vary significantly between ethnic groups. The Bihari community constitutes one of the most vulnerable populations in Bangladesh on the basis of access to health services and other fundamental rights. Our study aimed at finding out the prevalence and risk factors of type 2 diabetes among the Bihari adults in Dhaka city. Methods This cross-sectional community-based study was carried out among stranded Pakistanis (known as Bihari) living in camps in the Mirpur area from July 2014 to June 2015. Laboratory-based oral glucose tolerance test (OGTT) was the basis for the diagnosis of type 2 diabetes mellitus (DM). Anthropometric measurements, blood pressure, biochemical tests, family history, and socioeconomic information were obtained to determine the risk factors. Results The prevalence of diabetes mellitus (DM), impaired glucose tolerance (IGT), and impaired fasting glucose (IFG) were estimated at 10.11%, 8.74%, and 4.55%, respectively. Increased diastolic blood pressure, serum triglyceride, and cholesterol level were observed to be significantly (p

• Rousham, Emily K; Unicomb, Leanne; Wood, Paul; Smith, Michael; Asaduzzaman, Muhammad & Islam, Mohammad Aminul (2018). Spatial and temporal variation in the community prevalence of antibiotic resistance in Bangladesh: an integrated surveillance study protocol. BMJ Open. ISSN 2044-6055. 8. doi: 10.1136/bmjopen-2018-023158. Fulltekst i vitenarkiv Vis sammendrag

  Introduction Increasing antibiotic resistance (ABR) in low-income and middle-income countries such as Bangladesh presents a major health threat. However, assessing the scale of the health risk is problematic in the absence of reliable data on the community prevalence of antibiotic-resistant bacteria. We describe the protocol for a small-scale integrated surveillance programme that aims to quantify the prevalence of colonisation with antibiotic-resistant bacteria and concentrations of antibiotic-resistant genes from a ‘One Health’ perspective. The holistic assessment of ABR in humans, animals and within the environment in urban and rural Bangladesh will generate comprehensive data to inform human health risk. Methods and analysis The study design focuses on three exposure-relevant sites where there is enhanced potential for transmission of ABR between humans, animals and the environment: (1) rural poultry-owning households, (2) commercial poultry farms and (3) urban live-bird markets. The comparison of ABR prevalence in human groups with high and low exposure to farming and poultry will enable us to test the hypothesis that ABR bacteria and genes from the environment and food-producing animals are potential sources of transmission to humans. Escherichia coli is used as an ABR indicator organism due to its widespread environmental presence and colonisation in both the human and animal gastrointestinal tract.

• Flowra, Mahfuza Talukder & Asaduzzaman, Muhammad (2018). Resurgence of infectious diseases due to forced migration: is planetary health and One Health action synergistic? The Lancet Planetary Health. ISSN 2542-5196. 2(10), s. e419–e420. doi: 10.1016/S2542-5196(18)30203-1. Vis sammendrag

  Forced migration is an ancient phenomenon and will continue as long as politics exists. Although it mainly evolves from persecution, development, or exploitation, its public health consequences and effects go far beyond assumption. The ongoing epidemic of a rare and controlled disease like diphtheria in Rohingya camps, in Bangladesh, is a unique example of a public health disaster due to forced migration. Planetary health and One Health have approached the issue by working together as an interdisciplinary collaboration; which might be the best strategy in such conditions.

• Asaduzzaman, Muhammad (2018). Antimicrobial resistance: an urgent need for a planetary and ecosystem approach. The Lancet Planetary Health. ISSN 2542-5196. 2(3), s. e99–e100. doi: 10.1016/S2542-5196(18)30019-6. Vis sammendrag

  Our planet is rushing towards a post-antibiotic era and the global burden of antimicrobial resistance is estimated to rise to 10 million annual deaths by 2050, a figure that can substantially increase if adequate measures are not taken. Antimicrobial resistance is an ancient but complex phenomenon that emerges in response to antimicrobial exposure. The abundance of existing scientific publications on antimicrobial resistance in an environmental context supports its inclusion in the planetary health agenda. Unfortunately, antimicrobial resistance is still a public health concern and it has not been considered a priority area to work on from an ecological perspective. Ecologists and Earth scientists have not been made aware of this situation and have not been included in discussions of control strategies between public health experts and social scientists.

• Khan, Ashraful Islam; Ali, Mohammad; Chowdhury, Fahima; Saha, Amit; Khan, Iqbal Ansary & Khan, Arifuzzaman [Vis alle 15 forfattere av denne artikkelen] (2017). Safety of the oral cholera vaccine in pregnancy: Retrospective findings from a subgroup following mass vaccination campaign in Dhaka, Bangladesh. Vaccine. ISSN 0264-410X. doi: 10.1016/j.vaccine.2017.01.080. Fulltekst i vitenarkiv Vis sammendrag

  BACKGROUND: Pregnant women are vulnerable to complications of cholera. Killed oral cholera vaccines (OCV) are not recommended for pregnant women though there is no evidence of harmful effects during pregnancy. We evaluated the effect of a killed OCV, Shanchol™, on pregnancy outcomes during an effectiveness trial of the vaccine in urban Bangladesh. METHODOLOGY: Individuals ⩾1year were invited to participate in the trial, conducted in 2011 in Dhaka, Bangladesh. Pregnancy by history was an exclusion criterion and all women of reproductive age (15-49years) were verbally questioned about pregnancy at enrollment and prior to vaccination. Out of 48,414 women of reproductive age 286 women received the OCV unknowingly while pregnant. Out of these, we could recruit 69 women defined as exposed to OCV. Accordingly, we selected 69 pregnant women randomly from those who did not take the OCV (non-exposed to OCV). We evaluated adverse pregnancy outcome (spontaneous miscarriages, still births, or congenital malformations) between those who were exposed to OCV and those who were not exposed to OCV. RESULTS: About 16% of pregnant women exposed to OCV had pregnancy loss, as compared to 12% of unvaccinated pregnant women (P=0.38). One congenital anomaly was observed and occurred in women non-exposed to OCV group. Models that adjusted for baseline characteristics that were unbalanced between the exposed and non-exposed groups, revealed a no elevation of risk of adverse pregnancy outcomes in vaccinees versus non-vaccinees (Adj. OR (95% CI): 0.45 (0.11-1.88). CONCLUSIONS: No excess of adverse fetal outcomes associated with receipt of OCV was observed in this study. TRIAL REGISTRATION: Clinical Trials.gov number NCT01339845.

• Al‐Mamun, Md Mahfuz; Sarker, Suprovath Kumar; Qadri, Syeda Kashfi; Shirin, Tahmina; Mohammad, Quazi Deen & LaRocque, Regina [Vis alle 11 forfattere av denne artikkelen] (2016). Examination of Huntington's disease with atypical clinical features in a Bangladeshi family tree. Clinical Case Reports. ISSN 2050-0904. 4(12), s. 1191–1194. doi: 10.1002/ccr3.743. Fulltekst i vitenarkiv Vis sammendrag

  Atypical manifestation of Huntington's disease (HD) could inform ongoing research into HD genetic modifiers not present in the primarily European populations studied to date. This work demonstrates that expanding HD genetic testing into under-resourced healthcare settings can benefit both local communities and ongoing research into HD etiology and new therapies. Here, we describe an extended family in northern Bangladesh with eight members affected with Huntington's disease (HD) exhibiting atypical clinical features. All eight have the CAG repeat expansion in exon 1 of HTT gene and do not carry mutations associated with other HD‐like disorders, Wilson's disease, or other neurodegenerative diseases. The affected family, in northern Bangladesh, has eight living members with Huntington's disease (HD), all with atypical clinical features. The disorder appeared almost 130 years ago in one of the two siblings. All affected individuals are progeny of the affected sibling, and no progeny of the healthy sibling are affected. No consanguineous marriages are reported for the study participants.

• Qadri, Dr Firdausi; Wierzba, Thomas F.; Ali, Mohammad; Chowdhury, Fahima; Khan, Ashraful Islam & Saha, Amit [Vis alle 27 forfattere av denne artikkelen] (2016). Efficacy of a Single-Dose, Inactivated Oral Cholera Vaccine in Bangladesh. New England Journal of Medicine. ISSN 0028-4793. 374, s. 1723–1732. doi: 10.1056/NEJMoa1510330. Fulltekst i vitenarkiv Vis sammendrag

  Background A single-dose regimen of the current killed oral cholera vaccines that have been prequalified by the World Health Organization would make them more attractive for use against endemic and epidemic cholera. We conducted an efficacy trial of a single dose of the killed oral cholera vaccine Shanchol, which is currently given in a two-dose schedule, in an urban area in which cholera is highly endemic. Methods Nonpregnant residents of Dhaka, Bangladesh, who were 1 year of age or older were randomly assigned to receive a single dose of oral cholera vaccine or oral placebo. The primary outcome was vaccine protective efficacy against culture-confirmed cholera occurring 7 to 180 days after dosing. Prespecified secondary outcomes included protective efficacy against severely dehydrating culture-confirmed cholera during the same interval, against cholera and severe cholera occurring 7 to 90 versus 91 to 180 days after dosing, and against cholera and severe cholera according to age at baseline. Results A total of 101 episodes of cholera, 37 associated with severe dehydration, were detected among the 204,700 persons who received one dose of vaccine or placebo. The vaccine protective efficacy was 40% (95% confidence interval [CI], 11 to 60%; 0.37 cases per 1000 vaccine recipients vs. 0.62 cases per 1000 placebo recipients) against all cholera episodes, 63% (95% CI, 24 to 82%; 0.10 vs. 0.26 cases per 1000 recipients) against severely dehydrating cholera episodes, and 63% (95% CI, −39 to 90%), 56% (95% CI, 16 to 77%), and 16% (95% CI, −49% to 53%) against all cholera episodes among persons vaccinated at the age of 5 to 14 years, 15 or more years, and 1 to 4 years, respectively, although the differences according to age were not significant (P=0.25). Adverse events occurred at similar frequencies in the two groups. Conclusions A single dose of the oral cholera vaccine was efficacious in older children (≥5 years of age) and in adults in a setting with a high level of cholera endemicity. (Funded by the Bill and Melinda Gates Foundation and others; ClinicalTrials.gov number, NCT02027207. opens in new tab.)

• Chowdhury, Fahima; Mather, Alison E.; Begum, Yasmin Ara; Asaduzzaman, Muhammad; Baby, Nabilah & Sharmin, Salma [Vis alle 15 forfattere av denne artikkelen] (2015). Vibrio cholerae Serogroup O139: Isolation from Cholera Patients and Asymptomatic Household Family Members in Bangladesh between 2013 and 2014. PLoS Neglected Tropical Diseases. ISSN 1935-2727. 9(11). doi: 10.1371/journal.pntd.0004183. Fulltekst i vitenarkiv Vis sammendrag

  Background Cholera is endemic in Bangladesh, with outbreaks reported annually. Currently, the majorityof epidemic cholera reported globally is El Tor biotypeVibrio choleraeisolates of the ser-ogroup O1. However, in Bangladesh, outbreaks attributed toV.choleraeserogroup O139isolates, which fall within the same phylogenetic lineage as the O1 serogroup isolates, wereseen between 1992 and 1993 and in 2002 to 2005. Since then,V.choleraeserogroup O139has only been sporadically isolated in Bangladesh and is now rarely isolated elsewhere. Methods Here, we present case histories of four cholera patients infected withV.choleraeserogroupO139 in 2013 and 2014 in Bangladesh. We comprehensively typed these isolates usingconventional approaches, as well as by whole genome sequencing. Phenotypic typing andPCR confirmed all four isolates belonging to the O139 serogroup. Findings Whole genome sequencing revealed that three of the isolates were phylogenetically closely related to previously sequenced El Tor biotype, pandemic 7, toxigenicV.choleraeO139 iso-lates originating from Bangladesh and elsewhere. The fourth isolate was a non-toxigenic V.cholerae that, by conventional approaches, typed as O139 serogroup but was genetically divergent from previously sequenced pandemic 7V.cholerae lineages belonging to the O139 or O1 serogroups. Conclusion These results suggest that previously observed lineages of V.choleraeO139 persist in Bangladesh and can cause clinical disease and that a novel disease-causing non-toxigenicO139 isolate also occurs.

• Qadri, Dr Firdausi; Ali, Mohammad; Chowdhury, Fahima; Khan, Ashraful Islam; Saha, Amit & Khan, Iqbal Ansary [Vis alle 28 forfattere av denne artikkelen] (2015). Feasibility and effectiveness of oral cholera vaccine in an urban endemic setting in Bangladesh: a cluster randomised open-label trial. The Lancet. ISSN 0140-6736. 386(10001), s. 1362–1371. doi: 10.1016/S0140-6736(15)61140-0. Vis sammendrag

  Background Cholera is endemic in Bangladesh with epidemics occurring each year. The decision to use a cheap oral killed whole-cell cholera vaccine to control the disease depends on the feasibility and effectiveness of vaccination when delivered in a public health setting. We therefore assessed the feasibility and protective effect of delivering such a vaccine through routine government services in urban Bangladesh and evaluated the benefit of adding behavioural interventions to encourage safe drinking water and hand washing to vaccination in this setting. Methods We did this cluster-randomised open-label trial in Dhaka, Bangladesh. We randomly assigned 90 clusters (1:1:1) to vaccination only, vaccination and behavioural change, or no intervention. The primary outcome was overall protective effectiveness, assessed as the risk of severely dehydrating cholera during 2 years after vaccination for all individuals present at time of the second dose. This study is registered with ClinicalTrials.gov, number NCT01339845. Findings Of 268 896 people present at baseline, we analysed 267 270: 94 675 assigned to vaccination only, 92 539 assigned to vaccination and behavioural change, and 80 056 assigned to non-intervention. Vaccine coverage was 65% in the vaccination only group and 66% in the vaccination and behavioural change group. Overall protective effectiveness was 37% (95% CI lower bound 18%; p=0·002) in the vaccination group and 45% (95% CI lower bound 24%; p=0·001) in the vaccination and behavioural change group. We recorded no vaccine-related serious adverse events. Interpretation Our findings provide the first indication of the effect of delivering an oral killed whole-cell cholera vaccine to poor urban populations with endemic cholera using routine government services and will help policy makers to formulate vaccination strategies to reduce the burden of severely dehydrating cholera in such populations. Funding Bill & Melinda Gates Foundation .

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• Asaduzzaman, Muhammad; Khai, Tual Sawn; Claro, Vergil de & Zaman, Farzana (2023). Global Disparities in COVID-19 Vaccine Distribution: A Call for More Integrated Approaches to Address Inequities in Emerging Health Challenges. Challenges. ISSN 2078-1547. 14(4). doi: 10.3390/challe14040045. Fulltekst i vitenarkiv Vis sammendrag

  The advent of the COVID-19 vaccine signified a historic milestone in the field of vaccinology, showcasing remarkable scientific collaboration and global solidarity. However, the most prominent hurdle in maximizing the global public health impact of vaccines remains the absence of comprehensive and inclusive health systems in both high- and low-resource settings. Our discussion centers around the major contributing factors that played a key role in formulating the rapid and efficacious COVID-19 vaccines. Simultaneously, we illuminate the disparities that have marred the vaccine delivery process throughout the pandemic. In particular, we highlight the case scenarios of two minority and vulnerable communities from the Global South and North—the undocumented migrants in Thailand and the Roma community in Europe—who continue to experience inequitable vaccine access regardless of their location. We note that a crucial programmatic solution that is rooted in inclusive and equitable global public health policy, characterized by empathy and trust and bolstered by digital innovation, is lacking. These examples underscore the significance of establishing a comprehensive and integrated health system at multiple levels across countries and the entire world. Furthermore, we highlight the need for both local and global actors to collaboratively engage in vaccine distribution efforts. By gaining a concise grasp of these intricacies, the global community will be better poised to effectively combat future pandemics and emerging health challenges.

• Moura, Pedro; Collineau, Lucie; Sandberg, Marianne; Tomassone, Laura; De Meneghi, Daniele & Norström, Madelaine [Vis alle 16 forfattere av denne artikkelen] (2023). Users’ perception of the OH-EpiCap evaluation tool based on its application to nine national antimicrobial resistance surveillance systems. Frontiers in Public Health. ISSN 2296-2565. 11. doi: 10.3389/fpubh.2023.1138645.
• Asaduzzaman, Muhammad (2022). Neo-colonialism in Global Health:Perspectives from VIRDE Alumni and Evidence from the Field.
• Asaduzzaman, Muhammad; Rose, Elizabeth S. & Boafor, Theodore Kobla (2022). Understanding Implications of Research Colonialism to Make Change for the Future.
• Asaduzzaman, Muhammad (2022). Water and food in One Health perspective.
• Asaduzzaman, Muhammad; Rødland, Ernst Kristian; Mekonnen, Zeleke; Gradmann, Christoph & Winkler, Andrea Sylvia (2022). Understanding transmission pathways and integrated digital surveillance potential of antimicrobial resistance in Ethiopia in a One Health approach: a mixed-method study protocol. BMJ Open. ISSN 2044-6055. 12(6). doi: 10.1136/bmjopen-2021-051022. Fulltekst i vitenarkiv Vis sammendrag

  Introduction Antimicrobial resistance (AMR) has a critical global impact, mostly affecting low- and middle-income countries. A major knowledge gap exists in understanding the transmission pathway of the gut colonisation with AMR bacteria between healthy humans and their animals in addition to the presence of those AMR bacteria in the surrounding environment. A One Health (OH) approach is necessary to address this multisectoral problem. Methods and analysis This cross-sectional, mixed-method OH study design will use both quantitative and qualitative methods of data collection. Quantitative methods will be carried out to assess the prevalence and risk factors associated with multidrug resistant Gram-negative bacteria and vancomycin-resistant enterococci in humans, animals (cattle) and the environment. The focus will be on cattle rearing as an exposure risk for AMR among humans. The assessment of AMR in the population of Jimma, Ethiopia with or without exposure to cattle will reinforce the importance of OH research to identify the impending exchange of resistance profile between humans and animals as well as its ultimate dissemination in the surrounding environment. The targeted semistructured key stakeholder interviews will aid to strengthen the OH-AMR surveillance in Ethiopia by understanding the acceptability of an integrated AMR surveillance platform based on the District Health Information Software-2 and the feasibility of its context-specific establishment. Ethics and dissemination The study has been approved by the Regional Ethics Committee, Norway, and the Institutional Review Board of Jimma University, Ethiopia. The study’s data will be stored on a secure server known as Services for Sensitive Data hosted by the University of Oslo. In addition, the new European Union Global Data Protection Guidelines for data sharing, storage and protection will be followed. We will publish the results in peer-reviewed journals and present the findings at national and international conferences.

• Asaduzzaman, Muhammad (2022). How can we reduce the use of antibiotics in food production? Vis sammendrag

  Antimicrobial resistance (AMR) is one of the greatest threats to modern healthcare and is arguably the most multidisciplinary global health issue we face. By leveraging the purchasing power of healthcare, we can support an antibiotic-free supply chain for food and promote the reduction of antibiotic misuse in farming practices. While antimicrobials, or more specifically antibiotics, have revolutionized contemporary medical science and significantly reduced both mortality and morbidity, we now know that their misuse and overuse are fueling the emergence of resistant ‘superbugs’. The development and containment of AMR are complex ‘One Health’ issues. As healthcare professionals, we have a vital role to play in achieving One Health goals and are among the many actors who need to collaborate and embrace further responsibilities beyond our routine tasks. One major driver of AMR is the disproportionate use of antibiotics in food animals, which consume 50-80% of antibiotics in developed countries. Global figures from 2013 estimated that 131,109 tons of antibiotics were consumed by food animals and projected that this would reach 200,235 tons by 2030. Animal farming and agricultural production contribute to a larger extent for the antibiotic residues and metabolites entering the environment, along with hospital wastewater, pharmaceutical effluents, and sewage. As a healthcare professional and global health researcher, I believe we need to focus on the less explored, but impactful role of healthcare’s purchasing power. Leveraging our spending, healthcare institutions can support an antibiotic free supply chain for food in healthcare settings and promote the reduction of overused and misused antibiotics in food animal farming.

• Asaduzzaman, Muhammad; Rahman, K M Saif-Ur- & Ahmed, Sabrina (2021). Prevention of future pandemics needs attention to planetary health: the example of bat ecology and COVID-19. Medium.
• Asaduzzaman, Muhammad (2021). One Health - Interdisciplinary Research Approach in Global Health .
• Asaduzzaman, Muhammad (2021). Water and Food in One Health perspective.
• Asaduzzaman, Muhammad (2020). Think About Climate Change and Pandemics While Washing Your Hands. Medium. Vis sammendrag

  The need for access to safe water, sanitation and hygiene has proven to be of the utmost importance in order to safeguard human health, especially amidst the ongoing COVID-19 pandemic. These three symbiotic terms are collectively known as WASH. Among them, hand-washing is the most commonly recommended measure to prevent the spread of disease. My concern is about the link between WASH and climate change as well as how understanding this planetary issue is essential in order to prepare for future pandemics. I believe that WASH is important to the planetary health agenda, particularly for urban populations.

• Asaduzzaman, Muhammad & Murshid, Munzur-E- (2020). Climate Change and Vulnerability to Emerging Infections: Nature’s Revenge or Our Fate! Medium. Vis sammendrag

  The whole world is at standstill due to the COVID-19 pandemic. Vehicles are not running, planes are not flying, factories are closed and even social gatherings are halted. The world has not been faced with such great danger since World War II. The COVID-19 crisis has demonstrated how helpless we truly are. After the COVID-19 pandemic, the question arose as to whether climate change has anything to do with the emergence of such pandemics. The question has not yet been fully answered. However, scientists have identified various factors that have been linked to the emergence of viral diseases

• Asaduzzaman, Muhammad; Rousham, Emily K & Zaman, Farzana (2020). Antibiotic consumption may be linked to exaggeration of COVID-19. Medical Hypotheses. ISSN 0306-9877. 143. doi: 10.1016/j.mehy.2020.109913. Vis sammendrag

  When we observe the history of the deadliest pandemics on earth from Antonine Plague to current COVID-19, no bacterial pandemic has occurred since the discovery of Penicillin. The misuse of antibiotics in respiratory viral infections is well established. We hypothesize that use of antibiotics is linked with the exacerbation of viral infections like COVID-19 on the basis of epidemiological and immunological evidence.

• Storeng, Katerini Tagmatarchi; Roalkvam, Sidsel & Asaduzzaman, Muhammad (2019). Action to protect the independence and integrity of global health research. BMJ Global Health. ISSN 2059-7908. 4. doi: 10.1136/%20bmjgh-2019-001746. Vis sammendrag

  The tensions between research ethics and the wider politics of the global health field are increasingly recognised. However, the repercussions of these tensions for individuals and research institutions need careful consideration. While ‘rocking the boat’ is uncomfortable and may threaten individual career progression and research institutions’ external income, biased evidence can harm health programme beneficiaries and public trust in research. There are certainly no simple, fail-safe, technocratic quick fixes to resolving issues of power and politics, but the ideas proposed here should at least create better relationships between the institutions involved in commissioning, undertaking and publishing research, and feed into more sophisticated and thoughtful mechanisms of accountability, which do not simply re-enforce existing frameworks that favour accountability towards donors. The ideas we propose should be considered within broader discussions on how to address north–south power imbalances within the research community, and will hopefully catalyse wider action on protecting the independence of public universities and other research institutions globally. We believe this is necessary to enable researchers to hold power to account and advance informed and healthy debate on issues of public interest.

• Asaduzzaman, Muhammad (2019). Antimicrobial Resistance – The value of Global One Health Studies.
• Asaduzzaman, Muhammad & Ahmed, Niyaz (2018). F1000 Prime Recommendation of "A poor-quality generic drug for the treatment of visceral leishmaniasis: a case report and appeal". F1000Prime. doi: 10.3410/f.720295206.793549590.
• Asaduzzaman, Muhammad & Ahmed, Niyaz (2017). F1000 Prime recommendation of "Evolution of antibiotic resistance is linked to any genetic mechanism affecting bacterial duration of carriage". F1000Prime. doi: 10.3410/f.727215364.793535058.
• Asaduzzaman, Muhammad & Ahmed, Niyaz (2017). F1000 Prime Recommendation of "Interconnected microbiomes and resistomes in low-income human habitats". F1000Prime. doi: 10.3410/f.726354064.793532883. Vis sammendrag

  This important research article highlights a very significant pathway of the spread of antibiotic resistance among humans, animals and the environment by horizontal gene transfer between bacterial genomes including benign gut microbiota and environmental pathogens. This article reports the identification of interconnected resistomes from human faecal samples and the surrounding environment (including feaces of domesticated animals, soil, water as well as samples from composting latrines and sewage systems) which are exclusively influenced by bacterial phylogeny along ecological gradients. Due to the selection of low income rural and peri-urban communities as study areas, these findings are relevant for two-thirds of the global population. The authors also focused on safe water, improved sanitation and waste management as an effective intervention to reduce the burden of antibiotic resistance.

• Asaduzzaman, Muhammad (2017). Living in the era of Superbugs: Curse and Remedy. Vis sammendrag

  Antimicrobial resistance (AMR) is one of the biggest threats to global health, food security and development for which the environment is recognised as an important actor. It is estimated that the annual AMR attributed death toll will reach 10 million by 2050. Poultry is a key driver of AMR in its transmission between the outdoor environment and humans through faecal shedding of resistant bacteria. The rapid expansion of commercial poultry farming in peri-urban areas creates risk due to the unregulated use of antibiotics in feed and disposal of faecal waste and run-off water. Strict biosecurity practices are absent, especially in developing countries. Regulation, aimed at the poultry farm industry, must be implemented and enforced to decrease the growing problem of AMR.

• Flowra, Mahfuza Talukder & Asaduzzaman, Muhammad (2016). PREVALENCE AND ASSOCIATED RISK FACTORS FOR HYPERTENSION IN A MINOR ETHNIC GROUP (BIHARI COMMUNITY) IN BANGLADESH. Vis sammendrag

  Objective: This study is the first one to determine the prevalence and associated risk factors for Hypertension among the Bihari community of Bangladesh, a neglected minor ethnic group of population living in different camps with poor facilities for more than four decades. Being poor and illiterate, they are also unaware of the dangers of major NCDs. So, it is important to know the current situation of Hypertension and other NCDs of this community. Design and Method: This cross-sectional study was conducted among subjects, aged ≥ 30 years in Bihari camp at Mirpur of Dhaka city. Along with Blood pressure measurement, OGTT, anthropometric measurements and biochemical tests were obtained. Stata 13 was used for data entry and analysis. Statistical comparisons were carried out using t-test and χ2 test. Logistic regression was applied to calculate crude and adjusted odds ratio to identify risk factors. Results: The prevalence of Hypertension was 7.03% in this study group. Prevalence of Hypertension was greater in females than males. Increased blood pressure was significantly (p < 0.05) associated with type 2 DM, increased Triglyceride and Cholesterol level. It has been also observed that positive family history of DM and high BP along with obesity significantly affected the probability of Hypertension. The higher waist circumference had significant (p < 0.05) association with high BP in both sex. Conclusions: The prevalence of Hypertension in the study population was found to be almost equal to that of the general population of Bangladesh. Therefore, the policy makers should initiate cardiovascular care with health education in the minor ethnic groups and whatever the causes, they must be included in the country's health policy regarding development of prevention and control model of noncommunicable diseases.

• Asaduzzaman, Muhammad & Mahmood, Sultan (2016). Epidemiology, impact, prevention and control of nipah encephalitis in Bangladesh: a systematic review of one health issue. European Journal of Epidemiology (EJE). ISSN 0393-2990. 31. doi: 10.1007/s10654-016-0183-1. Vis sammendrag

  Background Nipah virus poses an eminent threat in Bangladesh as it encounters almost yearly outbreak since 2001. Comprehensive and coherent understanding of the disease is of paramount importance due to high case fatality. The objective of this review is to summarize the epidemiology and control of Nipah encephalitis in Bangladesh. Methods We searched three different databases- PubMed, Web of Science and Google Scholar using different search term as appropriate for those databases mixing the Boolean operators that yielded 144, 113 and 1530 articles respectively. We screened the titles first for relevance and later abstracts. 47 articles met our criteria and were finally reviewed. A literature matrix was formed in Excel for compilation and analysis. Results Nipah outbreaks in Bangladesh were different compared to initial outbreak in Malaysia in 1999. Unlike Malaysian outbreak, person to person transmission occurred in Bangladesh leaving humans most vulnerable. Infections were reported in domesticated pigs, dogs, cats in South Asia and serological study in Bangladesh showed 6.5, 4.3 and 44.2 %seroprevalence in cattle, goat and pig respectively. Pteropus bat plays a key role in Nipah transmission and risk factors include drinking raw date palm sap contaminated with bat urine or faeces, eating halfeaten bat saliva laden fruit and close contact or involvement in burial of cases. As date palm sap is harvested during winter, Nipah outbreaks mostly occurred during this season. Nipah occurred within the ‘‘Nipah belt’’ and had a high case fatality of more than 70 %. Avoiding raw date palm sap and drinking sap from trees protected with bamboo skirt or ‘‘bana’’ remain the principle preventive measures along with strict infection control in hospitals for prevention of secondary cases. Conclusion Extensive knowledge of this emerging zoonosis is the key for prevention and saving lives. Community sensitization and multisectoral intervention through One Health approach can prevent this deadly disease especially in outbreak areas.

• Asaduzzaman, Muhammad & Mahmood, Sultan (2016). Epidemiology, impact, prevention and control of nipah encephalitis in Bangladesh: a systematic review of one health issue.

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