Organoids and OoC systems can be used for e.g. studying drug metabolism and as disease models, as a complementary approach to animal models. The Wilson team employs mass spectrometry (MS) to study organoids and OoC systems, as MS provides highly sensitive and accurate identifications of chemicals present in a sample. MS can be used to identify and measure small drugs, metabolites/lipids, proteins, etc., enabling pharmacokinetic studies and omics. Prior to MS, a separation step is often required, so that a plethora of compounds can be measured from the same sample, but not overloading the MS instrument. The separation is typically performed using liquid chromatography (LC).
The Wilson team specialized in the development and application of combining organoids/OoC with LC-MS, often in an online/automated format. Research areas include studying the liver´s metabolism of drugs of abuse, disease models such as non-alcoholic fatty liver disease (NAFLD), and insulin production in lab-grown islets.
Selected Publications:
Simultaneous LC-MS determination of glucose regulatory peptides secreted by stem cell-derived islet organoids.
Olsen C, Wang C, Aizenshtadt A, Abadpour S , Lundanes E, Skottvoll FS, Golovin A, Busek M, Krauss S, Scholz H, Wilson SR. Electrophoresis. 2023. PMID: 37574258.
Mass spectrometry reveals that oxysterols are secreted from non-alcoholic fatty liver disease induced organoids.
Kømurcu KS, Wilhelmsen I, Thorne JL , Krauss S, Wilson SR, Aizenshtadt A, Røberg-Larsen H. J Steroid Biochem Mol Biol. 2023. PMID: 37380087.
"Organ-in-a-Column" Coupled On-line with Liquid Chromatography-Mass Spectrometry.
Kogler S, Aizenshtadt A, Harrison S, Skottvoll FS, Berg HE, Abadpour S, Scholz H, Sullivan G, Thiede B, Lundanes E, Bogen IL, Krauss S, Røberg-Larsen H, Wilson SR. Anal Chem. 2022. PMID: 36484723.
Direct Electromembrane Extraction-Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids.
Skottvoll FS, Aizenshtadt A, Hansen FA, Martinez MA, Andersen JM, Bogen IL, Kutter JP, Pedersen-Bjergaard S, Lundanes E, Krauss S, Wilson SR. Analysis & Sensing 21. DOI: 10.1002/anse.202100051.
Electromembrane Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies.
Skottvoll FS, Hansen FA, Harrison S, Boger IS, Mrsa A, Restan MS, Stein M, Lundanes E, Pedersen-Bjergaard S, Aizenshtadt A, Krauss S, Sullivan G, Bogen IL, Wilson SR. Anal Chem. 2021. PMID: 33534551.
