Maria Schüller's thesis, titled; Determination of liver organoid- and spheroid induced drug metabolites using liquid chromatography-mass spectrometry, aimed to develop a selective and sensitive nanoLC-MS method for the liver toxicity assessment of drug candidates utilizing the organ-emulating power of organoids.
Ida Caroline Sneis Boger's thesis, titled; Determination of liver organoid- and spheroid induced drug metabolites using liquid chromatography-mass spectrometry, investigated the metabolizing properties of liver organoids and spheroids. She exposed the organoids and spheroids to a drug, monitored the resulting drug metabolism using LC-MS, and compared the metabolism to traditional in vitro drug metabolism models such as liver fractions and hepatocytes.
Stian Kogler's thesis, titled; On-line “Organ-in-a-Column” Liquid Chromatography Electrospray Ionization Mass Spectrometry for Drug Metabolism Studies, he developed the novel concept of "Organ-in-a-column" where empty high performance liquid chromatography column housings were used to incubate organoids with subsequent online separation and detection of metabolites. With this online system, automated drug metabolism studies were performed on hepatic organoids showing that the oranoids would adapt to prolonged drug exposure.
Stian also won an award for best master thesis at the Department of Chemistry.
Congratulations to all three!