Western laboratory

Waste management of

• Acrylamide gels/solutions
• Beta-mercaptoethanol/dithiothreitol (DTT)
• SDS
• Solutions containing methanol
• Kits
• NaN3 solutions (Na-azide)
• Plastic equipment
• Bromphenol blue

Protective Equipment

• Gloves
• Laboratory coat
• Safety glasses

Procedure for disposal

• Allow the acrylamide solution to polymerize before disposing of it in yellow toxic waste containers (polymerize: i.e. add TEMED + APS).
• Solutions that contain 0.1% ß-mercaptoethanol or more must be collected in labelled plastic bottles and handled as hazardous waste.
• Pipette tips used for ß-mercaptoethanol must be collected in a closed container and handled as hazardous waste.
• Solutions that contain 3% methanol or more must be handled as hazardous waste.
• Contaminated plastic equipment must be disposed of in yellow toxic waste containers.
• Solutions that contain 0.1% Na-azide or more must be collected in plastic bottles and handled as hazardous waste.
• Kits: waste must be handled according to the HSE data sheet that comes with the kit.