Andreas Lossius

• Polak, Justyna; Wagnerberger, Johanna Henny; Torsetnes, Silje Bøen; Lindeman, Ida; Høglund, Rune Alexander Aamodt & Vartdal, Frode [Show all 8 contributors for this article] (2023). Single-cell transcriptomics combined with proteomics of intrathecal IgG reveal transcriptional heterogeneity of oligoclonal IgG-secreting cells in multiple sclerosis. Frontiers in Cellular Neuroscience. ISSN 1662-5102. 17. doi: 10.3389/fncel.2023.1189709. Full text in Research Archive
• Lindeman, Ida; Polak, Justyna; Qiao, Shuo-Wang; Holmøy, Trygve; Høglund, Rune Alexander & Vartdal, Frode [Show all 9 contributors for this article] (2022). Stereotyped B-cell responses are linked to IgG constant region polymorphisms in multiple sclerosis. European Journal of Immunology. ISSN 0014-2980. 52(4), p. 550–565. doi: 10.1002/eji.202149576. Show summary

  Clonally related B cells infiltrate the brain, meninges, and cerebrospinal fluid of MS patients, but the mechanisms driving the B-cell response and shaping the immunoglobulin repertoires remain unclear. Here, we used single-cell full-length RNA-seq and BCR reconstruction to simultaneously assess the phenotypes, isotypes, constant region polymorphisms, and the paired heavy- and light-chain repertoires in intrathecal B cells. We detected extensive clonal connections between the memory B cell and antibody-secreting cell (ASC) compartments and observed clonally related cells of different isotypes including IgM/IgG1, IgG1/IgA1, IgG1/IgG2, and IgM/IgA1. There was a strong dominance of the G1m1 allotype constant region polymorphisms in ASCs, but not in memory B cells. Tightly linked to the G1m1 allotype, we found a preferential pairing of the immunoglobulin heavy-chain variable (IGHV)4 gene family with the κ variable (IGKV)1 gene family. The IGHV4-39 gene was most used and showed the highest frequency of pairing with IGKV1-5 and IGKV1(D)-33. These results link IgG constant region polymorphisms to stereotyped B-cell responses in MS and indicate that the intrathecal B-cell response in these patients could be directed against structurally similar epitopes

• Amoriello, Roberta; Chernigovskaia, Mariia; Greiff, Victor; Carnasciali, Alberto; Massacesi, Luca & Barilaro, Alessandro [Show all 13 contributors for this article] (2021). TCR repertoire diversity in Multiple Sclerosis: High-dimensional bioinformatics analysis of sequences from brain, cerebrospinal fluid and peripheral blood. EBioMedicine. ISSN 2352-3964. 68:103429, p. 1–13. doi: 10.1016/j.ebiom.2021.103429. Full text in Research Archive
• Høglund, Rune Alexander; Bremel, Robert; Homan, E. Jane; Torsetnes, Silje Bøen; Lossius, Andreas & Holmøy, Trygve (2020). CD4+ T Cells in the Blood of MS Patients Respond to Predicted Epitopes From B cell Receptors Found in Spinal Fluid. Frontiers in Immunology. ISSN 1664-3224. 11, p. 1–12. doi: 10.3389/fimmu.2020.00598. Full text in Research Archive
• Høglund, Rune Alexander; Torsetnes, Silje Bøen; Lossius, Peter Andreas Vold; Bogen, Bjarne; Homan, E. Jane & Bremel, Robert [Show all 7 contributors for this article] (2019). Human cysteine cathepsins degrade immunoglobulin G in vitro in a predictable manner. International Journal of Molecular Sciences. ISSN 1661-6596. 20:4843(19), p. 1–19. doi: 10.3390/ijms20194843. Full text in Research Archive
• Tomescu-Baciu, Alina; Johansen, Jorunn; Holmøy, Trygve; Greiff, Victor; Stensland, Maria & De Souza, Gustavo Antonio [Show all 8 contributors for this article] (2019). Persistence of intrathecal oligoclonal B cells and IgG in multiple sclerosis. Journal of Neuroimmunology. ISSN 0165-5728. 333:576966, p. 1–7. doi: 10.1016/j.jneuroim.2019.576966. Full text in Research Archive Show summary

  In multiple sclerosis (MS), B cells are trafficking across the blood-brain barrier, but it is not known how this relates to the synthesis of oligoclonal IgG. We used quantitative mass spectrometry of oligoclonal bands and high-throughput sequencing of immunoglobulin heavy-chain variable transcripts to study the longitudinal B cell response in the cerebrospinal fluid (CSF) and blood of two MS patients. Twenty of 22 (91%) and 25 of 28 (89%) of oligoclonal band peptides persisted in samples collected 18 months apart, in spite of a dynamic exchange across the blood-CSF barrier of B lineage cells connecting to oligoclonal IgG.

• Myhr, Kjell-Morten; Torkildsen, Øivind; Lossius, Peter Andreas Vold; Bø, Lars & Holmøy, Trygve (2019). B cell depletion in the treatment of multiple sclerosis. Expert Opinion on Biological Therapy. ISSN 1471-2598. 19(3), p. 261–271. doi: 10.1080/14712598.2019.1568407.
• Tomescu-Baciu, Alina; Vartdal, Frode; Holmøy, Trygve; Vedeler, Christian A. & Lossius, Peter Andreas Vold (2018). G1m1 predominance of intrathecal virus-specific antibodies in multiple sclerosis. Annals of clinical and translational neurology. ISSN 2328-9503. 5(10), p. 1303–1309. doi: 10.1002/acn3.642. Full text in Research Archive
• Høglund, Rune Alexander; Polak, Justyna; Vartdal, Frode; Holmøy, Trygve & Lossius, Peter Andreas Vold (2018). B-cell composition in the blood and cerebrospinal fluid of multiple sclerosis patients treated with dimethyl fumarate. Multiple Sclerosis and Related Disorders. ISSN 2211-0348. 26, p. 90–95. doi: 10.1016/j.msard.2018.08.032. Full text in Research Archive
• Høglund, Rune Alexander; Lossius, Peter Andreas Vold; Johansen, Jorunn; Homan, Jane; Saltyte Benth, Jurate & Robins, Harlan [Show all 9 contributors for this article] (2017). In silico prediction analysis of idiotope-driven T-B cell collaboration in multiple sclerosis. Frontiers in Immunology. ISSN 1664-3224. 8. doi: 10.3389/fimmu.2017.01255. Full text in Research Archive
• Lossius, Peter Andreas Vold; Tomescu-Baciu, Alina; Holmøy, Trygve; Vedeler, Christian A.; Røsjø, Egil Rørvik & Lorentzen, Åslaug Rudjord [Show all 8 contributors for this article] (2017). Selective intrathecal enrichment of G1m1-positive B cells in multiple sclerosis. Annals of clinical and translational neurology. ISSN 2328-9503. 4(10), p. 756–761. doi: 10.1002/acn3.451. Full text in Research Archive
• Røsjø, Egil Rørvik; Lossius, Peter Andreas Vold; Abdelmagid, Nada; Lindstrøm, Jonas Christoffer; Kampman, Margitta Theodora & Jørgensen, Lone [Show all 11 contributors for this article] (2017). Effect of high-dose vitamin D3 supplementation on antibody responses against Epstein-Barr virus in relapsing-remitting multiple sclerosis. Multiple Sclerosis. ISSN 1352-4585. 23(3), p. 395–402. doi: 10.1177/1352458516654310.
• Lossius, Peter Andreas Vold; Johansen, Jorunn; Vartdal, Frode & Holmøy, Trygve (2016). High-throughput sequencing of immune repertoires in multiple sclerosis. Annals of clinical and translational neurology. ISSN 2328-9503. 3(4), p. 295–306. doi: 10.1002/acn3.295. Full text in Research Archive
• Johansen, Jorunn; Vartdal, Frode; Desmarais, Cindy; Tutturen, Astrid Elisabeth Voorham; De Souza, Gustavo Antonio & Lossius, Peter Andreas Vold [Show all 7 contributors for this article] (2015). Intrathecal BCR transcriptome in multiple sclerosis versus other neuroinflammation: Equally diverse and compartmentalized, but more mutated, biased and overlapping with the proteome. Clinical Immunology. ISSN 1521-6616. 160(2), p. 211–225. doi: 10.1016/j.clim.2015.06.001.
• Løken-Amsrud, Kristin Ingeleiv; Lossius, Peter Andreas Vold; Torkildsen, Øivind & Holmøy, Trygve (2015). Miljøets betydning ved multippel sklerose. Tidsskrift for Den norske legeforening. ISSN 0029-2001. 135(9), p. 856–860. doi: 10.4045/tidsskr.14.0751.
• Wesnes, Kristin; Riise, Trond; Casetta, Ilaria; Drulovic, Jelena; Granieri, Enrico & Holmøy, Trygve [Show all 16 contributors for this article] (2015). Body size and the risk of multiple sclerosis in Norway and Italy: The EnvIMS study. Multiple Sclerosis. ISSN 1352-4585. 21(4), p. 388–395. doi: 10.1177/1352458514546785.
• Lossius, Peter Andreas Vold; Johansen, Jorunn; Vartdal, Frode; Robins, Harlan; Saltyte Benth, Jurate & Holmøy, Trygve [Show all 7 contributors for this article] (2014). High-throughput sequencing of TCR repertoires in multiple sclerosis reveals intrathecal enrichment of EBV-reactive CD8+ T cells. European Journal of Immunology. ISSN 0014-2980. 44(11), p. 3439–3452. doi: 10.1002/eji.201444662.
• Bjørnevik, Kjetil Lauvland; Riise, Trond; Casetta, Ilaria; Drulovic, Jelena; Granieri, Enrico & Holmøy, Trygve [Show all 16 contributors for this article] (2014). Sun exposure and multiple sclerosis risk in Norway and Italy: The EnvIMS study. Multiple Sclerosis. ISSN 1352-4585. 20(8), p. 1042–1049. doi: 10.1177/1352458513513968.
• Lossius, Peter Andreas Vold; Riise, Trond; Pugliatti, Maura; Bjørnevik, Kjetil Lauvland; Casetta, Ilaria & Drulovic, Jelena [Show all 16 contributors for this article] (2014). Season of infectious mononucleosis and risk of multiple sclerosis at different latitudes; the EnvIMS Study. Multiple Sclerosis. ISSN 1352-4585. 20(6), p. 669–674. doi: 10.1177/1352458513505693.
• Holmøy, Trygve; Lossius, Peter Andreas Vold; Gundersen, Thomas Erik; Moen, Stine Marit; Castellazzi, Massimilano & Fainardi, Enrico [Show all 7 contributors for this article] (2012). Intrathecal levels of vitamin D and IgG in multiple sclerosis. Acta Neurologica Scandinavica. ISSN 0001-6314. 125(6), p. e28–e31. doi: 10.1111/j.1600-0404.2011.01577.x.
• Vold, Lossius Peter Andreas; Johansen, Jorunn; Torkildsen, Øivind; Vartdal, Frode & Holmøy, Trygve (2012). Epstein-Barr Virus in Systemic Lupus Erythematosus, Rheumatoid Arthritis and Multiple Sclerosis—Association and Causation. Viruses. ISSN 1999-4915. 4(12), p. 3701–3730. doi: 10.3390/v4123701.
• Lossius, Peter Andreas Vold; Vartdal, Frode & Holmøy, Trygve (2011). Vitamin D sensitive EBNA-1 specific T cells in the cerebrospinal fluid of patients with multiple sclerosis. Journal of Neuroimmunology. ISSN 0165-5728. 240, p. 87–96. doi: 10.1016/j.jneuroim.2011.09.011.

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• Gilmour, Brian Christopher; Chan, Hanna; Borowicz, Pawel; Phuyal, Santosh; Lossius, Andreas & Spurkland, Anne (2023). β2, or not β2? Connecting the Lck-adaptor TSAd to integrin β2 via a combined wet lab & bioinformatic approach.
• Gilmour, Brian Christopher; Chan, Hanna; Borowicz, Pawel; Johan Georg, Visser; Lossius, Andreas & Spurkland, Anne (2022). Applying bioinformatics to pick-apart elusive protein functions: Focusing on TSAd/SH2D2A, an immune kinase adaptor.

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