In vivo two-photon laser scanning microscopes

This unit allows in vivo brain imaging of behaving animals with options for experimental perturbations using chemical activiation and optogenetics.

Equipment

We have two models of “Ultima IV” two-photon laser scanning microscopy stations, produced by Prairie Technologies, Inc., Middleton, WI (a Bruker Corporation).

The setups allow imaging in vivo – even on the behaving animal, active on, e. g. a track ball – and include high quality objectives, image scanners, rapid scanners (resonant galvanometric), and independently controllable scanners for chemical activation purposes and optogenetics.

The system includes up to four detectors of which at least three highly efficient GaAsP PMT tube detectors. The laser sources are group wave dispersion compensated ultra-fast laser units emitting wave lengths in the range 680nm–1080nm/1300nm.

The setups also include the data acquisition software (uses “Prairie View”), which, in coordination with the high-end user electronics, also allows for time coordinated image scanning, chemical activation and control of external processes.

Contact and booking

Booking

The systems are being moved and upgraded, and therefore temporarily unavailable for booking in 2024. 

Staff

  • Mahmood Amiry-Moghaddam
  • Rune Enger
  • Vidar Jensen

Contact

Pricing and Booking

Published June 25, 2023 12:01 AM - Last modified Apr. 2, 2024 12:52 PM