Chencheng Wang

• Hrušková, Helena; Olsen, Christine; Řemínek, Roman; Wang, Chencheng; Aizenshtadt, Aleksandra & Krauss, Stefan Johannes Karl [Vis alle 10 forfattere av denne artikkelen] (2024). Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin. Journal of Chromatography A. ISSN 0021-9673. 1717. doi: 10.1016/j.chroma.2024.464669.
• Aizenshtadt, Aleksandra; Wang, Chencheng; Abadpour, Shadab; Menezes, Pedro Duarte; Wilhelmsen, Ingrid & Dalmao-Fernandez, Andrea [Vis alle 15 forfattere av denne artikkelen] (2024). Pump-Less, Recirculating Organ-on-Chip (rOoC) Platform to Model the Metabolic Crosstalk between Islets and Liver. Advanced Healthcare Materials. ISSN 2192-2640. doi: 10.1002/adhm.202303785.
• Wang, Chencheng; Abadpour, Shadab; Olsen, Petter Angell; Wang, Daxin; Stokowiec, Justyna & Chera, Simona [Vis alle 11 forfattere av denne artikkelen] (2024). Glucose Concentration in Regulating Induced Pluripotent Stem Cells Differentiation Toward Insulin-Producing Cells. Transplant International. ISSN 0934-0874. 37. doi: 10.3389/ti.2024.11900.
• Olsen, Christine; Wang, Chencheng; Aizenshtadt, Aleksandra; Abadpour, Shadab; Lundanes, Elsa & Skottvoll, Frøydis Sved [Vis alle 11 forfattere av denne artikkelen] (2023). Simultaneous LC–MS determination of glucose regulatory peptides secreted by stem cell–derived islet organoids. Electrophoresis. ISSN 0173-0835. 44(21-22), s. 1682–1697. doi: 10.1002/elps.202300095. Fulltekst i vitenarkiv
• Wilhelmsen, Ingrid; Amirola Martinez, Mikel; Stokowiec, Justyna; Wang, Chencheng; Aizenshtadt, Aleksandra & Krauss, Stefan Johannes Karl (2023). Characterization of human stem cell-derived hepatic stellate cells and liver sinusoidal endothelial cells during extended in vitro culture. Frontiers in Bioengineering and Biotechnology. ISSN 2296-4185. 11. doi: 10.3389/fbioe.2023.1223737. Fulltekst i vitenarkiv
• Olsen, Christine; Wang, Chencheng; Abadpour, Shadab; Lundanes, Elsa; Hansen, Audun Skau & Skottvoll, Frøydis Sved [Vis alle 8 forfattere av denne artikkelen] (2022). Determination of insulin secretion from stem cell-derived islet organoids with liquid chromatography-tandem mass spectrometry. Journal of chromatography. B. ISSN 1570-0232. 1215. doi: 10.1016/j.jchromb.2022.123577. Fulltekst i vitenarkiv
• Kwak, Dongho; Combriat, Thomas Michel Daniel; Wang, Chencheng; Scholz, Hanne; Danielsen, Anne & Jensenius, Alexander Refsum (2022). Music for Cells? A Systematic Review of Studies Investigating the Effects of Audible Sound Played Through Speaker-Based Systems on Cell Cultures . Music & Science. ISSN 2059-2043. 5. doi: 10.1177/20592043221080965. Fulltekst i vitenarkiv Vis sammendrag

  There have been several studies investigating whether musical sound can be used as cell stimuli in recent years. We systematically searched publications to get an overview of studies that have used audible sound played through speaker-based systems to induce mechanical perturbation in cell cultures. A total of 12 studies were identified. We focused on the experimental setups, the sounds that were used as stimuli, and relevant biological outcomes. The studies are categorized into simple and complex sounds depending on the type of sound employed. Some of the promising effects reported were enhanced cell migration, proliferation, colony formation, and differentiation ability. However, there are significant differences in methodologies and cell type-specific outcomes, which made it difficult to find a systematic pattern in the results. We suggest that future experiments should consider using: (1) a more controlled acoustic environment, (2) standardized sound and noise measurement methods, and (3) a more comprehensive range of controlled sound parameters as cellular stimuli.

• Fernandez, Manuel Carrasco; Wang, Chencheng; Søviknes, Anne Mette; Bjørlykke, Yngvild; Abadpour, Shadab & Paulo, Joao A. [Vis alle 16 forfattere av denne artikkelen] (2022). Spatial Environment Affects HNF4A Mutation-Specific Proteome Signatures and Cellular Morphology in hiPSC-Derived β-Like Cells. Diabetes. ISSN 0012-1797. 71(4), s. 862–869. doi: 10.2337/db20-1279. Vis sammendrag

  Studies of monogenic diabetes are particularly useful because we can gain insight into the molecular events of pancreatic β-cell failure. Maturity-onset diabetes of the young 1 (MODY1) is a form of monogenic diabetes caused by a mutation in the HNF4A gene. Human-induced pluripotent stem cells (hiPSCs) provide an excellent tool for disease modeling by subsequently directing differentiation toward desired pancreatic islet cells, but cellular phenotypes in terminally differentiated cells are notoriously difficult to detect. Re-creating a spatial (three-dimensional [3D]) environment may facilitate phenotype detection. We studied MODY1 by using hiPSC-derived pancreatic β-like patient and isogenic control cell lines in two different 3D contexts. Using size-adjusted cell aggregates and alginate capsules, we show that the 3D context is critical to facilitating the detection of mutation-specific phenotypes. In 3D cell aggregates, we identified irregular cell clusters and lower levels of structural proteins by proteome analysis, whereas in 3D alginate capsules, we identified altered levels of glycolytic proteins in the glucose sensing apparatus by proteome analysis. Our study provides novel knowledge on normal and abnormal function of HNF4A, paving the way for translational studies of new drug targets that can be used in precision diabetes medicine in MODY.

• Abadpour, Shadab; Wang, Chencheng; Niemi, Essi Maria & Scholz, Hanne (2021). Tissue Engineering Strategies for Improving Beta Cell Transplantation Outcome. Current Transplantation Reports. 8(3), s. 205–219. doi: 10.1007/s40472-021-00333-2. Fulltekst i vitenarkiv

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• Wang, Chencheng; Abadpour, Shadab; Aizenshtadt, Alexandra; Chera, Simona; Ghila, Luiza Mihaela & Ræder, Helge [Vis alle 7 forfattere av denne artikkelen] (2021). Glucose during in vitro pancreatic beta cells regeneration: friends or for?
• Wang, Chencheng; Abadpour, Shadab; Aizenshtadt, Alexandra; Chera, Simona; Ghila, Luiza Mihaela & Ræder, Helge [Vis alle 7 forfattere av denne artikkelen] (2021). 402.2: High Glucose Concentration Increases KATP Channel Activity but Suppresses Mitochondrial Respiration Ability in Insulin-producing Cells Regenerated From Stem Cells. Transplantation. ISSN 0041-1337. s. S27–S27. doi: 10.1097/01.tp.0000804436.14096.44.

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