Andreas Romaine

• Sasi, Athiramol; Romaine, Andreas; Murugan Erusappan, Pugazendhi; Melleby, Arne Olav; Hasic, Almira & Dahl, Christen Peder [Show all 14 contributors for this article] (2023). Temporal expression and spatial distribution of the proteoglycan versican during cardiac fibrosis development. Matrix Biology Plus. 19-20. doi: 10.1016/j.mbplus.2023.100135. Full text in Research Archive
• Vistnes, Maria; Murugan Erusappan, Pugazendhi; Sasi, Athiramol; Nordén, Einar Sjaastad; Bergo, Kaja Knudsen & Romaine, Andreas [Show all 22 contributors for this article] (2023). Inhibition of the extracellular enzyme A disintegrin and metalloprotease with thrombospondin motif 4 prevents cardiac fibrosis and dysfunction. Cardiovascular Research (CVR). ISSN 0008-6363. 119(10), p. 1915–1927. doi: 10.1093/cvr/cvad078. Full text in Research Archive
• Romaine, Andreas; Melleby, Arne Olav; Alam, Jahedul; Lobert, Viola; Lu, Ning & Lockwood, Francesca Eve [Show all 13 contributors for this article] (2022). Integrin α11β1 and syndecan-4 dual receptor ablation attenuates cardiac hypertrophy in the pressure overloaded heart. American Journal of Physiology. Heart and Circulatory Physiology. ISSN 0363-6135. 322(6), p. H1057–H1071. doi: 10.1152/ajpheart.00635.2021.
• Alam, Jahedul; Musiime, Moses; Romaine, Andreas; Sawant, Mugdha; Melleby, Arne Olav & Lu, Ning [Show all 9 contributors for this article] (2020). Generation of a novel mouse strain with fibroblast-specific expression of Cre recombinase. Matrix Biology Plus. 8. doi: 10.1016/j.mbplus.2020.100045.
• Herum, Kate Louise Møller; Romaine, Andreas; Wang, Ariel; Melleby, Arne Olav; Strand, Mari Elen & Pacheco, Julian [Show all 16 contributors for this article] (2020). Syndecan-4 Protects the Heart From the Profibrotic Effects of Thrombin-Cleaved Osteopontin. Journal of the American Heart Association (JAHA). ISSN 2047-9980. 9(3). doi: 10.1161/JAHA.119.013518. Full text in Research Archive
• Mathiesen, Sabrina Bech; Lunde, Marianne; Aronsen, Jan Magnus; Romaine, Andreas; Kaupang, Anita & Martinsen, Marita [Show all 11 contributors for this article] (2019). The cardiac syndecan-4 interactome reveals a role for syndecan-4 in nuclear translocation of muscle LIM protein (MLP). Journal of Biological Chemistry. ISSN 0021-9258. 294(22), p. 8717–8731. doi: 10.1074/jbc.RA118.006423. Full text in Research Archive Show summary

  Costameres are signaling hubs at the sarcolemma and important contact points between the extracellular matrix and cell interior, sensing and transducing biomechanical signals into a cellular response. The transmembrane proteoglycan syndecan-4 localizes to these attachment points and has been shown to be important in the initial stages of cardiac remodeling, but its mechanistic function in the heart remains insufficiently understood. Here, we sought to map the cardiac interactome of syndecan-4 to better understand its function and downstream signaling mechanisms. By combining two different affinity purification methods with MS analysis, we found that the cardiac syndecan-4 interactome consists of 21 novel and 29 previously described interaction partners. Nine of the novel partners were further validated to bind syndecan-4 in HEK293 cells (i.e. CAVIN1/PTRF, CCT5, CDK9, EIF2S1, EIF4B, MPP7, PARVB, PFKM, and RASIP). We also found that 19 of the 50 interactome partners bind differently to syndecan-4 in the left ventricle lysate from aortic-banded heart failure (ABHF) rats compared with SHAM-operated animals. One of these partners was the well-known mechanotransducer muscle LIM protein (MLP), which showed direct and increased binding to syndecan-4 in ABHF. Nuclear translocation is important in MLP-mediated signaling, and we found less MLP in the nuclear-enriched fractions from syndecan-4-/- mouse left ventricles but increased nuclear MLP when syndecan-4 was overexpressed in a cardiomyocyte cell line. In the presence of a cell-permeable syndecan-4-MLP disruptor peptide, the nuclear MLP level was reduced. These findings suggest that syndecan-4 mediates nuclear translocation of MLP in the heart

• Melleby, Arne Olav; Romaine, Andreas; Aronsen, Jan Magnus; Veras, Ioanni; Zhang, Lili & Sjaastad, Ivar [Show all 8 contributors for this article] (2018). A novel method for high precision aortic constriction that allows for generation of specific cardiac phenotypes in mice. Cardiovascular Research (CVR). ISSN 0008-6363. doi: 10.1093/cvr/cvy141. Full text in Research Archive
• Romaine, Andreas; Sørensen, Ida Wiig; Zeltz, Cedric; Lu, Ning; Erusappan, Pugazendhi Murugan & Melleby, Arne Olav [Show all 15 contributors for this article] (2017). Overexpression of integrin α11 induces cardiac fibrosis in mice. Acta Physiologica Scandinavica. ISSN 0001-6772. 222:e13025(2), p. 1–17. doi: 10.1111/apha.12932.
• Melleby, Arne Olav; Strand, Mari Elen; Romaine, Andreas; Herum, Kate Louise Møller; Skrbic, Biljana & Dahl, Christen Peder [Show all 11 contributors for this article] (2016). The heparan sulfate proteoglycan glypican-6 is upregulated in the failing heart, and regulates cardiomyocyte growth through ERK1/2 Signaling. PLOS ONE. ISSN 1932-6203. 11(10). doi: 10.1371/journal.pone.0165079.

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• Romaine, Andreas; Sørensen, Ida Wiig; Melleby, Arne Olav; Zeltz, Cedric; Zhang, Lili & Bendiksen, Bård Andre [Show all 12 contributors for this article] (2016). Overexpression of integrin a11 induces cardiac hypertrophy and fibrosis.
• Romaine, Andreas; Herum, Kate Louise Møller; Sørensen, Ida Wiig; Hasic, Almira; Sjaastad, Ivar & Gullberg, Donald [Show all 7 contributors for this article] (2015). Syndecan-4 regulates profibrotic a11B1 integrin expression in the pressure-overloaded heart.
• Romaine, Andreas; Herum, Kate Louise Møller; Skrbic, Biljana; Sørensen, I.W.; Gullberg, Donald & Christensen, Geir Arve (2014). The role of cardiac fibroblast integrin 11ß1 and syndecan-4 interaction in myocardial fibrosis and myofibroblast differentiation.

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