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• Andersen, Maren Stolp; Leikfoss, Ingvild Sørum; Brorson, Ina Skaara; Cappelletti, Chiara; Bettencourt, Conceicao & Toft, Mathias [Show all 7 contributors for this article] (2023). Epigenome-wide association study of peripheral immune cell populations in Parkinson’s disease. NPJ Parkinson's Disease. ISSN 2373-8057. 9(1). doi: 10.1038/s41531-023-00594-x.
• Cappelletti, Chiara; Eriksson, Anna Maria; Brorson, Ina Skaara; Leikfoss, Ingvild Sørum; Glomstad Kråbøl, Oda & Høgestøl, Einar August [Show all 12 contributors for this article] (2022). Quantitative proteomics reveals protein dysregulation during T cell activation in multiple sclerosis patients compared to healthy controls. Clinical Proteomics. ISSN 1542-6416. 19(1), p. 1–17. doi: 10.1186/s12014-022-09361-1. Full text in Research Archive Show summary

  Background Multiple sclerosis (MS) is an autoimmune, neurodegenerative disorder with a strong genetic component that acts in a complex interaction with environmental factors for disease development. CD4+ T cells are pivotal players in MS pathogenesis, where peripherally activated T cells migrate to the central nervous system leading to demyelination and axonal degeneration. Through a proteomic approach, we aim at identifying dysregulated pathways in activated T cells from MS patients as compared to healthy controls. Methods CD4+ T cells were purified from peripheral blood from MS patients and healthy controls by magnetic separation. Cells were left unstimulated or stimulated in vitro through the TCR and costimulatory CD28 receptor for 24 h prior to sampling. Electrospray liquid chromatography-tandem mass spectrometry was used to measure protein abundances. Results Upon T cell activation the abundance of 1801 proteins was changed. Among these proteins, we observed an enrichment of proteins expressed by MS-susceptibility genes. When comparing protein abundances in T cell samples from healthy controls and MS patients, 18 and 33 proteins were differentially expressed in unstimulated and stimulated CD4+ T cells, respectively. Moreover, 353 and 304 proteins were identified as proteins exclusively induced upon T cell activation in healthy controls and MS patients, respectively and dysregulation of the Nur77 pathway was observed only in samples from MS patients. Conclusions Our study highlights the importance of CD4+ T cell activation for MS, as proteins that change in abundance upon T cell activation are enriched for proteins encoded by MS susceptibility genes. The results provide evidence for proteomic disturbances in T cell activation in MS, and pinpoint to dysregulation of the Nur77 pathway, a biological pathway known to limit aberrant effector T cell responses.

• Brorson, Ina Skaara; Eriksson, Anna Maria; Høgestøl, Einar August; Leikfoss, Ingvild Sørum; Harbo, Hanne-Cathrin Flinstad & Berge, Tone [Show all 8 contributors for this article] (2022). Global DNA methylation changes in treated and untreated MS patients measured over time. Journal of Neuroimmunology. ISSN 0165-5728. 364. doi: 10.1016/j.jneuroim.2022.577808. Full text in Research Archive Show summary

  Multiple sclerosis (MS) is an autoimmune, neurological disease. We investigated genome-wide DNA methylation profiles of CD4+ and CD8+ T cells from MS patients and healthy controls at baseline and a follow-up visit. Patients were all treatment-naïve at baseline, and either on treatment or remained untreated at the follow-up visit. MS patients show more changes in their T cell DNA methylation profiles as compared to healthy controls over time, with the most pronounced differences observed in the untreated MS patients. These findings underline the potential of DNA methylation as biomarkers in MS.

• Brorson, Ina Skaara; Eriksson, Anna Maria; Leikfoss, Ingvild Sørum; Vitelli, Valeria; Celius, Elisabeth Gulowsen & Luders, Torben [Show all 10 contributors for this article] (2020). CD8 + T cell gene expression analysis identifies differentially expressed genes between multiple sclerosis patients and healthy controls. Multiple Sclerosis Journal, Experimental, Translational and Clinical. ISSN 2055-2173. doi: 10.1177/2055217320978511. Full text in Research Archive
• Rhead, Brooke; Brorson, Ina Skaara; Berge, Tone; Adams, Cameron; Quach, H. & Moen, Stine Marit [Show all 18 contributors for this article] (2019). Increased DNA methylation of SLFN12 in CD4+ and CD8+ T cells from multiple sclerosis patients. PLOS ONE. ISSN 1932-6203. doi: 10.1371/journal.pone.0206511.
• Brorson, Ina Skaara; Eriksson, Anna Maria; Leikfoss, Ingvild Sørum; Celius, Elisabeth Gulowsen; Berg-Hansen, Pål & Barcellos, Lisa F [Show all 9 contributors for this article] (2019). No differential gene expression for CD4+ T cells of MS patients and healthy controls. Multiple Sclerosis Journal, Experimental, Translational and Clinical. ISSN 2055-2173. 5(2). doi: 10.1177/2055217319856903.
• Brorson, Ina Skaara; Eriksson, Anna; Leikfoss, Ingvild Sørum; Celius, Elisabeth Gulowsen; Berg-Hansen, Pål & Barcellos, Lisa F. [Show all 9 contributors for this article] (2019). No differential gene expression for CD4+ T cells of MS patients and healthy controls. Multiple Sclerosis Journal, Experimental, Translational and Clinical. ISSN 2055-2173. 5(2). doi: 10.1177/2055217319856903. Full text in Research Archive
• Berge, Tone; Eriksson, Anna; Brorson, Ina Skaara; Høgestøl, Einar August; Berg-Hansen, Pål & Døskeland, Anne Marie Simonne [Show all 10 contributors for this article] (2019). Quantitative proteomic analyses of CD4+ and CD8+ T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controls . Clinical Proteomics. ISSN 1542-6416. 16:19, p. 1–18. doi: 10.1186/s12014-019-9241-5. Full text in Research Archive
• Rhead, Brooke; Brorson, Ina Skaara; Berge, Tone; Adams, Cameron; Quach, Hong & Moen, Stine Marit [Show all 18 contributors for this article] (2018). Increased DNA methylation of SLFN12 in CD4+ and CD8+ T cells from multiple sclerosis patients. PLOS ONE. ISSN 1932-6203. 13(10). doi: 10.1371/journal.pone.0206511. Full text in Research Archive
• Berge, Tone; Leikfoss, Ingvild Sørum; Brorson, Ina Skaara; Bos, Steffan Daniel; Page, Christian & Gustavsen, Marte Wendel [Show all 13 contributors for this article] (2016). The multiple sclerosis susceptibility genes TAGAP and IL2RA are regulated by Vitamin D in CD4+ T cells. Genes and Immunity. ISSN 1466-4879. 17(2), p. 118–127. doi: 10.1038/gene.2015.61.
• Leikfoss, Ingvild Sørum; Keshari, Pankaj Kumar; Gustavsen, Marte Wendel; Bjølgerud, Anja; Brorson, Ina Skaara & Celius, Elisabeth Gulowsen [Show all 10 contributors for this article] (2015). Multiple sclerosis risk allele in CLEC16A acts as an expression quantitative trait locus for CLEC16A and SOCS1 in CD4+ T cells. PLOS ONE. ISSN 1932-6203. 10>e0132957(7). doi: 10.1371/journal.pone.0132957. Full text in Research Archive

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• Eriksson, Anna Maria; Brorson, Ina Skaara; Cappelletti, Chiara; Berge, Tone & Bos, Steffan Daniel (2020). Et dybdykk i de usynlige spillerne i MS.
• Bos, Steffan Daniel; Brorson, Ina Skaara; Eriksson, Anna; Leikfoss, Ingvild Sørum; Vitelli, Valeria & Celius, Elisabeth Gulowsen [Show all 10 contributors for this article] (2019). Differential gene expression analysis of CD8+ T cells from multiple sclerosis patients and healthy controls .
• Bos, Steffan Daniel; Brorson, Ina Skaara; Høgestøl, Einar August; Berge, Tone; Saez-Rodriguez, Julio & Uccelli, Antonio [Show all 9 contributors for this article] (2018). Multiple Sclerosis genetic burden score in a systems biology study of MS patients from four countries.
• Berge, Tone; Eriksson, Anna; Brorson, Ina Skaara; Høgestøl, Einar August; Harbo, Hanne Flinstad & Bos, Steffan Daniel [Show all 7 contributors for this article] (2018). Proteomic profiling of T cells from multiple sclerosis patients and healthy controls.
• Brorson, Ina Skaara; Rhead, Brooke; Berge, Tone; Adams, Cameron; Quach, Hong & Sangurdekar, Dipen [Show all 18 contributors for this article] (2018). SLFN12 is differentially methylated in multiple sclerosis CD4+ and CD8+ T cells .
• Berge, Tone; Brorson, Ina Skaara; Høgestøl, Einar August; Bos, Steffan Daniel; Harbo, Hanne Flinstad & Berven, Frode (2018). Proteomic profiling of CD4+ and CD8+ T cells from multiple sclerosis patients and healthy controls.
• Eriksson, Anna; Brorson, Ina Skaara; Høgestøl, Einar August; Bos, Steffan Daniel; Harbo, Hanne Flinstad & Berven, Frode [Show all 7 contributors for this article] (2018). Proteomic profiling of CD4+ T cells from MS patients show increased activity of T cell activation pathways (compared to healthy controls) .
• Berge, Tone; Eriksson, Anna; Brorson, Ina Skaara; Høgestøl, Einar August; Bos, Steffan Daniel & Harbo, Hanne Flinstad [Show all 7 contributors for this article] (2018). Proteomic profiling of CD4+ and CD8+ T cells from multiple sclerosis patients and healthy controls.
• Brorson, Ina Skaara; Rhead, Brooke; Berge, Tone; Lea, Rodney A.; Burnard, Sean & Scott, Rodney J. [Show all 10 contributors for this article] (2018). Differently methylated regions in multiple sclerosis CD4+ and CD8+ T cells .
• Rhead, Brooke; Brorson, Ina Skaara; Berge, Tone; Adams, Cameron; Quach, Hong & Sangurdekar, Dipen [Show all 15 contributors for this article] (2018). Differential DNA methylation and expression of SLFN12 in multiple sclerosis patients’ CD4+ and CD8+ T cells.
• Bos, Steffan Daniel; Brorson, Ina Skaara; Barcellos, Lisa F; Berge, Tone & Harbo, Hanne Flinstad (2017). Gene expression profiling of CD4+ T cells from multiple sclerosis patients and healthy controls.
• Brorson, Ina Skaara; Rhead, Brooke; Quach, Hong; Berg-Hansen, Pål; Berge, Tone & Harbo, Hanne Flinstad [Show all 8 contributors for this article] (2017). DNA methylation differences in CD4+ and CD8+ T cells of multiple sclerosis patients and healthy controls.
• Mo, Ingrid; Eriksson, Anna; Brorson, Ina Skaara; Ledsaak, Marit; Harbo, Hanne Flinstad & Gabrielsen, Odd Stokke [Show all 7 contributors for this article] (2017). Identifying protein interaction partners for DEXI - encoded by the dexamethasone-induced gene, DEXI .
• Fernanda, Kropf Correia; Brorson, Ina Skaara; Eriksson, Anna; Bos, Steffan Daniel; Harbo, Hanne Flinstad & Berge, Tone (2017). Molecular characterization of TAGAP – a GTPase-activating protein encoded by a multiple sclerosis susceptibility gene.
• Brorson, Ina Skaara; Eriksson, Anna; Baranzini, Sergio; Berge, Tone; Harbo, Hanne Flinstad & Bos, Steffan Daniel (2017). Gene expression in pathways enriched with MS associated risk loci.
• Brorson, Ina Skaara; Rhead, Brooke; Berge, Tone; Adams, Cameron; Quach, Hong & Sangurdekar, Dipen [Show all 18 contributors for this article] (2010). SLFN12 is differentially methylated in multiple sclerosis CD4+ and CD8+ T cells .
• Berge, Tone & Brorson, Ina Skaara (2015). Vitamin D responsiveness of multiple sclerosis assocaited genes in CD4+ T cells. 7Letras.

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