Johanne Kolvik von Ely Iversen

• Iversen, Johanne Kolvik von Ely; Kahrs, Birgitte Heiberg & Eggebø, Torbjørn Moe (2021). There are 4, not 7, cardinal movements in labor. American Journal of Obstetrics & Gynecology MFM (AJOG MFM). ISSN 2589-9333. 3(6). doi: 10.1016/j.ajogmf.2021.100436. Show summary

  The mechanics of labor describe the forces required for fetal descent, and the movements that the fetus must perform to overcome the resistance met by the maternal bony pelvis and soft tissue. The fetus negotiates the birth canal and rotational movements are necessary for descent. Anglo-American literature lists 7 cardinal movements, namely engagement, descent, flexion, internal rotation, extension, external rotation, and expulsion. German and older English literature lists only 4 rotational movements as the cardinal movements and excludes engagement, descent, and expulsion. We would argue that descent is the main purpose of the uterine powers and cardinal movements, a description of the rotational movements the fetal head and shoulders must perform to obtain descent. Ultrasound offers a historically unique opportunity for noninvasive, dynamic studies of the mechanics of labor. The information gathered by clinical examination and ultrasound should be integrated into clinical decision making.

• Kamel, Rasha; Negm, Sherif; Badr, Islam; Kahrs, Birgitte Heiberg; Eggebø, Torbjørn M. & Iversen, Johanne Kolvik von Ely (2021). Fetal head descent assessed by transabdominal ultrasound: a prospective observational study. American Journal of Obstetrics and Gynecology. ISSN 0002-9378. 226(1), p. 1–10. doi: 10.1016/j.ajog.2021.07.030. Full text in Research Archive Show summary

  Background Detection of copy number variation (CNV) in genes associated with disease is important in genetic diagnostics, and next generation sequencing (NGS) technology provides data that can be used for CNV detection. However, CNV detection based on NGS data is in general not often used in diagnostic labs as the data analysis is challenging, especially with data from targeted gene panels. Wet lab methods like MLPA (MRC Holland) are widely used, but are expensive, time consuming and have gene-specific limitations. Our aim has been to develop a bioinformatic tool for CNV detection from NGS data in medical genetic diagnostic samples. Results Our computational pipeline for detection of CNVs in NGS data from targeted gene panels utilizes coverage depth of the captured regions and calculates a copy number ratio score for each region. This is computed by comparing the mean coverage of the sample with the mean coverage of the same region in other samples, defined as a pool. The pipeline selects pools for comparison dynamically from previously sequenced samples, using the pool with an average coverage depth that is nearest to the one of the samples. A sliding window-based approach is used to analyze each region, where length of sliding window and sliding distance can be chosen dynamically to increase or decrease the resolution. This helps in detecting CNVs in small or partial exons. With this pipeline we have correctly identified the CNVs in 36 positive control samples, with sensitivity of 100% and specificity of 91%. We have detected whole gene level deletion/duplication, single/multi exonic level deletion/duplication, partial exonic deletion and mosaic deletion. Since its implementation in mid-2018 it has proven its diagnostic value with more than 45 CNV findings in routine tests. Conclusions With this pipeline as part of our diagnostic practices it is now possible to detect partial, single or multi-exonic, and intragenic CNVs in all genes in our target panel. This has helped our diagnostic lab to expand the portfolio of genes where we offer CNV detection, which previously was limited by the availability of MLPA kits.

• Iversen, Johanne Kolvik; Kahrs, Birgitte Heiberg; Torkildsen, Erik A. & Eggebø, Torbjørn Moe (2020). Fetal molding examined with transperineal ultrasound and associations with position and delivery mode. American Journal of Obstetrics and Gynecology. ISSN 0002-9378. 223(6), p. 909.E1–909.E8. doi: 10.1016/j.ajog.2020.06.042. Full text in Research Archive
• Iversen, Johanne Kolvik & Eggebø, Torbjørn Moe (2019). Increased diagnostic accuracy of fetal head station by use of transabdominal ultrasound. Acta Obstetricia et Gynecologica Scandinavica. ISSN 0001-6349. 98(6), p. 805–806. doi: 10.1111/aogs.13529.
• Iversen, Johanne Kolvik; Jacobsen, Anne Flem; Mikkelsen, Thea Falkenberg & Eggebø, Torbjørn Moe (2019). Structured clinical examinations in labor: rekindling the craft of obstetrics. Journal of Maternal-Fetal & Neonatal Medicine. ISSN 1476-7058. p. 1–8. doi: 10.1080/14767058.2019.1651283. Full text in Research Archive

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• Iversen, Johanne Kolvik & Eggebø, Torbjørn Moe (2019). Fetal station assessed with transabdominal ultrasound.
• Iversen, Johanne Kolvik & Eggebø, Torbjørn Moe (2018). Increased diagnostic accuracy of fetal head station by use of transabdominal ultrasound. Acta Obstetricia et Gynecologica Scandinavica. ISSN 0001-6349. doi: 10.1111/aogs.13529.

View all works in Cristin