Trine Baur Opstad

• Alehagen, Urban; Alexander, Jan; Aaseth, Jan; Larsson, Anders & Opstad, Trine Baur (2024). Supplementation with selenium and coenzyme Q<inf>10</inf> in an elderly Swedish population low in selenium — positive effects on thyroid hormones, cardiovascular mortality, and quality of life. BMC Medicine. ISSN 1741-7015. 22(1). doi: 10.1186/s12916-024-03411-1.
• Aaseth, Jan; Alehagen, Urban; Opstad, Trine Baur & Alexander, Jan (2023). Vitamin K and Calcium Chelation in Vascular Health. Biomedicines. 11(12). doi: 10.3390/biomedicines11123154. Full text in Research Archive Show summary

  The observation that the extent of artery calcification correlates with the degree of atherosclerosis was the background for the alternative treatment of cardiovascular disease with chelator ethylenediamine tetraacetate (EDTA). Recent studies have indicated that such chelation treatment has only marginal impact on the course of vascular disease. In contrast, endogenous calcium chelation with removal of calcium from the cardiovascular system paralleled by improved bone mineralization exerted, i.e., by matrix Gla protein (MGP) and osteocalcin, appears to significantly delay the development of cardiovascular diseases. After post-translational vitamin-K-dependent carboxylation of glutamic acid residues, MGP and other vitamin-K-dependent proteins (VKDPs) can chelate calcium through vicinal carboxyl groups. Dietary vitamin K is mainly provided in the form of phylloquinone from green leafy vegetables and as menaquinones from fermented foods. Here, we provide a review of clinical studies, addressing the role of vitamin K in cardiovascular diseases, and an overview of vitamin K kinetics and biological actions, including vitamin-K-dependent carboxylation and calcium chelation, as compared with the action of the exogenous (therapeutic) chelator EDTA. Consumption of vitamin-K-rich foods and/or use of vitamin K supplements appear to be a better preventive strategy than EDTA chelation for maintaining vascular health.

• Opstad, Trine Baur; Papotti, Bianca; Åkra, Sissel; Hansen, Charlotte Holst; Braathen, Bjørn & Tønnessen, Theis [Show all 8 contributors for this article] (2023). Sirtuin1, not NAMPT, possesses anti-inflammatory effects in epicardial, pericardial and subcutaneous adipose tissue in patients with CHD. Journal of Translational Medicine. ISSN 1479-5876. 21(1). doi: 10.1186/s12967-023-04518-4. Full text in Research Archive
• Alehagen, Urban; Alexander, Jan; Aaseth, Jan; Larsson, Anders; Svensson, Erland & Opstad, Trine Baur (2023). Effects of an Intervention with Selenium and Coenzyme Q10 on Five Selected Age-Related Biomarkers in Elderly Swedes Low in Selenium: Results That Point to an Anti-Ageing Effect—A Sub-Analysis of a Previous Prospective Double-Blind Placebo-Controlled Randomised Clinical Trial. Cells. ISSN 2073-4409. 12(13). doi: 10.3390/cells12131773. Full text in Research Archive Show summary

  Background: Ageing is associated with cardiovascular disease (CVD). As no single biomarker reflects the full ageing process, we aimed to investigate five CVD- and age-related markers and the effects of selenium and coenzyme Q10 intervention to elucidate the mechanisms that may influence the course of ageing. Methods: This is a sub-study of a previous prospective double-blind placebo-controlled randomized clinical trial that included 441 subjects low in selenium (mean age 77, 49% women). The active treatment group (n = 220) received 200 µg/day of selenium and 200 mg/day of coenzyme Q10, combined. Blood samples were collected at inclusion and after 48 months for measurements of the intercellular adhesion molecule (ICAM-1), adiponectin, leptin, stem cell factor (SCF) and osteoprotegerin (OPG), using ELISAs. Repeated measures of variance and ANCOVA evaluations were used to compare the two groups. In order to better understand and reduce the complexity of the relationship between the biomarkers and age, factor analyses and structural equation modelling (SEM) were performed, and a structural model is presented. Results: Correlation analyses of biomarker values at inclusion in relation to age, and relevant markers related to inflammation, endothelial dysfunction and fibrosis, demonstrated the biomarkers' association with these pathological processes; however, only ICAM1 and adiponectin were directly correlated with age. SEM analyses showed, however, that the biomarkers ICAM-1, adiponectin, SCF and OPG, but not leptin, all had significant associations with age and formed two independent structural factors, both significantly related to age. While no difference was observed at inclusion, the biomarkers were differently changed in the active treatment and placebo groups (decreasing and increasing levels, respectively) at 48 months (p ≤ 0.02 in all, adjusted), and in the SEM model, they showed an anti-ageing impact. Conclusions: Supplementation with selenium/Q10 influenced the analysed biomarkers in ways indicating an anti-ageing effect, and by applying SEM methodology, the interrelationships between two independent structural factors and age were validated.

• Opstad, Trine Baur; Alexander, Jan; Aaseth, Jan; Larsson, Anders; Seljeflot, Ingebjørg & Alehagen, Urban (2023). Increased SIRT1 Concentration Following Four Years of Selenium and Q10 Intervention Associated with Reduced Cardiovascular Mortality at 10-Year Follow-Up—Sub-Study of a Previous Prospective Double-Blind Placebo-Controlled Randomized Clinical Trial. Antioxidants. ISSN 2076-3921. 12(3). doi: 10.3390/antiox12030759. Full text in Research Archive Show summary

  Background: Selenium and coenzyme Q10 (SeQ10) possess antioxidant and anti-inflammatory properties, potentially mediated via Sirtuin1 (SIRT1). We aimed to investigate the influence of a SeQ10 intervention on SIRT1 concentration, with potential interactions with microRNAs. Methods: In this sub-study of a prospective double-blind placebo-controlled clinical trial, healthy subjects (mean age 76 years) were randomized to receive an active treatment (n = 165, combined 200 µg/day of Se and 200 mg/day of Q10) or a placebo (n = 161). SIRT1 concentration and microRNAs were measured with ELISA and PCR, respectively. Results: After four years, SIRT1 concentration was increased in the active treatment group, with mean (SD) ng/mL of 469 (436) vs. 252 (162), p < 0.001, and decreased in the placebo group, 190 (186) vs. 269 (172), p = 0.002, and the differences between the groups were significant (p = 0.006, adjusted). Those who suffered CV death during a 10-year follow-up (n = 25 and n = 52 in the active treatment and placebo groups, respectively) had significantly lower baseline SIRT1 concentrations compared to the survivors (p < 0.001). MiR-130a-3p was significantly downregulated during the intervention and correlated inversely with SIRT1 at baseline (r = −0.466, p = 0.007). Conclusion: The increased SIRT1 concentration after the SeQ10 intervention associated with reduced CV mortality, partly mediated via miR-1303a-3p, suggests that SIRT1 is an additional mediator of the intervention, preventing vascular ageing.

• Papotti, Bianca; Opstad, Trine Baur; Åkra, Sissel; Tønnessen, Theis; Braathen, Bjørn & Hansen, Charlotte Holst [Show all 10 contributors for this article] (2023). Macrophage polarization markers in subcutaneous, pericardial, and epicardial adipose tissue are altered in patients with coronary heart disease. Frontiers in Cardiovascular Medicine. ISSN 2297-055X. 10. doi: 10.3389/fcvm.2023.1055069. Full text in Research Archive
• Kluge, Karsten Engseth; Langseth, Miriam Sjåstad; Andersen, Geir Øystein; Halvorsen, Sigrun; Opstad, Trine Baur & Arnesen, Harald [Show all 9 contributors for this article] (2022). Complement activation in association with clinical outcomes in ST-elevation myocardial infarction. American Heart Journal Plus: Cardiology Research and Practice (AHJ Plus). 24. doi: 10.1016/j.ahjo.2022.100228. Full text in Research Archive
• Opstad, Trine Baur; Nordeng, Jostein; Pettersen, Alf Åge Reistad; Åkra, Sissel; Bratset, Vibeke & Zaidi, Syed Hani Ali [Show all 9 contributors for this article] (2022). The NLRP3 Genetic Variant (rs10754555) Reduces the Risk of Adverse Outcome in Middle-Aged Patients with Chronic Coronary Syndrome. Journal of Immunology Research. ISSN 2314-8861. 2022. doi: 10.1155/2022/2366695. Full text in Research Archive Show summary

  Background. Inflammation is central in development of cardiovascular disease (CVD). Aberrant function of the Nod-Like Receptor Protein 3 (NLRP3) inflammasome, a central mediator in the proinflammatory response, has been associated with atherosclerosis. The influence of genetic determinants on this inflammatory pathway and its downstream effects is less known. We aimed to investigate the frequency of a single NLRP3 gene variant according to clinical outcome in CVD and its influence on NLRP3-related markers. Methods. In this observational study, we included 1001 patients with chronic coronary syndrome. Blood samples were drawn at inclusion, including whole-blood and PAXgene tubes for DNA and RNA isolation, respectively. Allelic discrimination of the NLRP3 single nucleotide polymorphism rs10754555 was performed; and gene expression of NLRP3, Toll-Like Receptor 4, Interleukin- (IL-) 1β, and IL-18 was relatively quantified, both methods by RT-PCR. Circulating IL-6, high-sensitivity (hs) C-reactive protein, IL-18, and IL-12 were measured by enzyme-like immunosorbent assays. Clinical endpoints during 2 years () were a composite of unstable angina pectoris, myocardial infarction, nonhemorrhagic stroke, and death. Results. Minor allele frequency of the NLRP3 variant was 0.36. In all, no association of the NLRP3 variant with clinical subgroups or outcome was found, neither any significant influence on the genes’ mRNA expression or circulating protein. However, in years (25 percentile), the variant G-allele is associated with significant lower risk of suffering a composite event ( (95% CI 0.19, 0.97), , adjusted). In the same age group, the NLRP3 gene was accordingly downregulated in G-allele carriers vs. noncarriers, and circulating IL12 was significantly reduced (, both). In years, no significant effect of the variant was observed. Conclusion. The age-related reduced risk of composite endpoint in rs10754555 G-allele carriers accompanied by diminished NLRP3 mRNA expression is hypothesis generating and needs to be further explored. The study is registered at http://www.clinicaltrials.gov, with identification number NCT00222261.

• Warlo, Ellen Mathea Kirsch; Bratset, Vibeke; Pettersen, Alf Åge Reistad; Holme, Pål Andre; Arnesen, Harald & Seljeflot, Ingebjørg [Show all 7 contributors for this article] (2022). Genetic variation in ADAMTS13 is related to VWF levels, atrial fibrillation and cerebral ischemic events. Clinical and applied thrombosis/hemostasis. ISSN 1076-0296. 28, p. 1–8. doi: 10.1177/10760296221141893. Full text in Research Archive
• Opstad, Trine Baur; Farup, Per Grønaas; Rootwelt, Helge & Aaseth, Jan (2022). Changes in circulating sirtuin 1 after bariatric surgery. NMCD. Nutrition Metabolism and Cardiovascular Diseases. ISSN 0939-4753. 32(12), p. 2858–2864. doi: 10.1016/j.numecd.2022.09.009. Full text in Research Archive Show summary

  Obesity is associated with chronic inflammation and oxidative stress. Weight loss after bariatric surgery improves the inflammatory state and risk of cardiovascular disease. Improvement in metabolic dysfunction might be associated with changes in the activity of sirtuin 1 (SIRT1) and we aimed to investigate the effect of bariatric surgery on its circulating levels. Methods and results This is a sub-study of a prospective cohort study, including 110 subjects with morbid obesity. The surgical procedure was either laparoscopic Roux-en-Y gastric bypass (RYGB) or sleeve gastrectomy (SG). Blood was sampled at inclusion and six and 12 months after surgery. SIRT1 was measured in EDTA plasma with an enzyme-linked immunosorbent assay. The mean age in the population was 43 years, 80% were women and mean body mass index (BMI) was 38.8 kg/m2. RYGB and SG were performed in 89 and 21 subjects, respectively. SIRT1 concentration was significantly reduced from baseline to six and 12 months after surgery, with mean values (SD) 156.8 (82.6), 119.5 (65.6) and 94.9 (45.6) ng/mL, respectively, (p ≤ 0.002, all), accompanied by significant reductions in C-reactive protein (CRP), BMI and triglycerides from inclusion (p < 0.001, all). Type of surgery did not differently modify SIRT1 levels (p = 0.09). CRP and triglycerides were both positively predictive of SIRT1 levels (p ≤ 0.001, both). Conclusion SIRT1 concentration was significantly lower six and 12 months after bariatric surgery. CRP and triglycerides independently predicted SIRT1 levels, suggesting that reduction in SIRT1 levels might not intrinsically be related to weight reduction, but to improvement in metaflammation. ª 2022 The Author(s). Published by Elsevier B.V. on behalf of The Italian Diabetes Society, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition and the Department of Clinical Medicine and Surgery, Federico II University. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)

• Opstad, Trine Baur; Solheim, Svein; Pettersen, Alf Åge Reistad; Kalstad, Are; Arnesen, Harald & Seljeflot, Ingebjørg (2022). TERT and TET2 Genetic Variants Affect Leukocyte Telomere Length and Clinical Outcome in Coronary Artery Disease Patients—A Possible Link to Clonal Hematopoiesis. Biomedicines. 10(8). doi: 10.3390/biomedicines10082027. Full text in Research Archive
• Opstad, Trine Baur; Alexander, Jan; Aaseth, Jan; Larsson, Anders; Seljeflot, Ingebjørg & Alehagen, Urban (2022). Selenium and Coenzyme Q10 Intervention Prevents Telomere Attrition, with Association to Reduced Cardiovascular Mortality-Sub-Study of a Randomized Clinical Trial. Nutrients. ISSN 2072-6643. 14(16). doi: 10.3390/nu14163346. Full text in Research Archive Show summary

  Abstract Short telomeres have been associated with ageing and cardiovascular disease. The influence on leukocyte telomere length (LTL) of long-term intervention with combined selenium and coenzyme Q10 is unknown. Our aim was to determine whether 42 months of selenium and coenzyme Q10 supplementation prevented telomere attrition and further cardiovascular mortality. The investigation is an explorative sub-study of a double-blind, placebo-controlled, randomized trial. Swedish citizens low in selenium (n = 118), aged 70–80 years, were included. Intervention time was 4 years, with 10 years’ follow-up time. LTL was relatively quantified with PCR at baseline and after 42 months. At baseline, LTL (SD) was 0.954 (0.260) in the active treatment group and 1.018 (0.317) in the placebo group (p = 0.23). At 42 months, less shortening of LTL was observed after active treatment compared with placebo (+0.019 vs. −0.129, respectively, p = 0.02), with a significant difference in change basing the analysis on individual changes in LTL (p &lt; 0.001). Subjects suffering future death presented with significantly shorter LTL at 42 months than survivors [0.791 (0.190) vs. 0.941 (0.279), p = 0.01], with a significant difference in change of LTL according to cardiovascular mortality and survival (p = 0.03). To conclude, preservation of LTL after selenium and coenzyme Q10 supplementation associated with reduced cardiovascular mortality.

• Zaidi, Syed Hani Ali; Aksnes, Tonje Amb; Åkra, Sissel; Eggesbø, Heidi Beate; Byrkjeland, Rune & Seljeflot, Ingebjørg [Show all 7 contributors for this article] (2022). Abdominal Adipose Tissue Associates With Adiponectin and TNFα in Middle-Aged Healthy Men. Frontiers in Endocrinology. ISSN 1664-2392. 13, p. 1–8. doi: 10.3389/fendo.2022.874977. Full text in Research Archive
• Aukrust, Sverre Grøver; Holte, Kristine Bech; Opstad, Trine Baur; Seljeflot, Ingebjørg; Berg, Tore Julsrud & Helseth, Ragnhild (2022). NETosis in Long-Term Type 1 Diabetes Mellitus and Its Link to Coronary Artery Disease. Frontiers in Immunology. ISSN 1664-3224. 12, p. 1–9. doi: 10.3389/fimmu.2021.799539. Full text in Research Archive
• Opstad, Trine Baur; Sundfør, Tine Mejlbo; Tonstad, Serena & Seljeflot, Ingebjørg (2021). Effect of intermittent and continuous caloric restriction on Sirtuin1 concentration depends on sex and body mass index. NMCD. Nutrition Metabolism and Cardiovascular Diseases. ISSN 0939-4753. doi: 10.1016/j.numecd.2021.03.005. Full text in Research Archive
• Zaidi, Hani; Byrkjeland, Rune; Unhammer Njerve, Ida; Åkra, Sissel; Solheim, Svein & Arnesen, Harald [Show all 8 contributors for this article] (2021). Adiponectin in relation to exercise and physical performance in patients with type 2 diabetes and coronary artery disease. Adipocyte. ISSN 2162-3945. 10(1), p. 612–620. doi: 10.1080/21623945.2021.1996699. Full text in Research Archive
• Alehagen, Urban; Opstad, Trine B.; Alexander, Jan; Larsson, Anders & Aaseth, Jan (2021). Impact of Selenium on Biomarkers and Clinical Aspects Related to Ageing. A Review. Biomolecules. ISSN 2218-273X. 11(10). doi: 10.3390/biom11101478. Full text in Research Archive Show summary

  Selenium (Se) is an essential dietary trace element that plays an important role in the prevention of inflammation, cardiovascular diseases, infections, and cancer. Selenoproteins contain selenocysteine in the active center and include, i.a., the enzymes thioredoxin reductases (TXNRD1–3), glutathione peroxidases (GPX1–4 and GPX6) and methionine sulfoxide reductase, involved in immune functions, metabolic homeostasis, and antioxidant defense. Ageing is an inevitable process, which, i.a., involves an imbalance between antioxidative defense and reactive oxygen species (ROS), changes in protein and mitochondrial renewal, telomere attrition, cellular senescence, epigenetic alterations, and stem cell exhaustion. These conditions are associated with mild to moderate inflammation, which always accompanies the process of ageing and age-related diseases. In older individuals, Se, by being a component in protective enzymes, operates by decreasing ROS-mediated inflammation, removing misfolded proteins, decreasing DNA damage, and promoting telomere length. Se-dependent GPX1–4 and TXNRD1–3 directly suppress oxidative stress. Selenoprotein H in the cell nucleus protects DNA, and selenoproteins residing in the endoplasmic reticulum (ER) assist in the removal of misfolded proteins and protection against ER stress. In this review, we highlight the role of adequate Se status for human ageing and prevention of age-related diseases, and further its proposed role in preservation of telomere length in middle-aged and elderly individuals.

• Kalstad, Are; Myhre, Peder L.; Laake, Kristian; Opstad, Trine B.; Tveit, Arnljot & Solheim, Svein [Show all 8 contributors for this article] (2021). Biomarkers of ageing and cardiac remodeling are associated with atrial fibrillation. Scandinavian Cardiovascular Journal. ISSN 1401-7431. 55(4), p. 213–219. doi: 10.1080/14017431.2021.1889653. Full text in Research Archive
• Opstad, Trine Baur; Berg, Tore Julsrud; Holte, Kristine Bech; Arnesen, Harald; Solheim, Svein & Seljeflot, Ingebjørg (2020). Reduced leukocyte telomere lengths and sirtuin 1 gene expression in long-term survivors of type 1 diabetes: A Dialong substudy. Journal of Diabetes Investigation. ISSN 2040-1116. p. 1–10. doi: 10.1111/jdi.13470. Full text in Research Archive
• Opstad, Trine Baur; Kalstad, Are; Holte, Kristine Bech; Berg, Tore Julsrud; Solheim, Svein & Arnesen, Harald [Show all 7 contributors for this article] (2020). Shorter leukocyte telomer length in healthy relatives of patients with coronary heart disease. Rejuvenation Research. ISSN 1549-1684. doi: 10.1089/rej.2019.2258.
• Langseth, Miriam Sjåstad; Helseth, Ragnhild; Ritschel, Vibeke Nordmo; Hansen, Charlotte Holst; Andersen, Geir Øystein & Eritsland, Jan [Show all 12 contributors for this article] (2020). Double-Stranded DNA and NETs Components in Relation to Clinical Outcome After ST-Elevation Myocardial Infarction. Scientific Reports. ISSN 2045-2322. 10(1). doi: 10.1038/s41598-020-61971-7. Full text in Research Archive
• Langseth, Miriam Sjåstad; Andersen, Geir Øystein; Husebye, Trygve Guttorm; Arnesen, Harald; Zucknick, Manuela & Solheim, Svein [Show all 10 contributors for this article] (2020). Neutrophil extracellular trap components and myocardial recovery in post-ischemic acute heart failure. PLOS ONE. ISSN 1932-6203. 15:e0241333(10), p. 1–14. doi: 10.1371/journal.pone.0241333. Full text in Research Archive
• Åkra, Sissel; Aksnes, Tonje Amb; Flaa, Arnljot; Eggesbø, Heidi Beate; Opstad, Trine Baur & Unhammer Njerve, Ida [Show all 7 contributors for this article] (2020). Markers of remodeling in subcutaneous adipose tissue are strongly associated with overweight and insulin sensitivity in healthy non-obese men. Scientific Reports. ISSN 2045-2322. 10:14055, p. 1–9. doi: 10.1038/s41598-020-71109-4. Full text in Research Archive
• Kluge, Karsten Engseth; Langseth, Miriam Sjåstad; Opstad, Trine Baur; Pettersen, Alf Åge Reistad; Arnesen, Harald & Tønnessen, Theis [Show all 8 contributors for this article] (2020). Complement Activation in Association with Markers of Neutrophil Extracellular Traps and Acute Myocardial Infarction in Stable Coronary Artery Disease. Mediators of Inflammation. ISSN 0962-9351. 2020. doi: 10.1155/2020/5080743. Full text in Research Archive
• Zaidi, Hani; Byrkjeland, Rune; Unhammer Njerve, Ida; Åkra, Sissel; Solheim, Svein & Arnesen, Harald [Show all 8 contributors for this article] (2019). Effects of exercise training on markers of adipose tissue remodeling in patients with coronary artery disease and type 2 diabetes mellitus: Sub study of the randomized controlled EXCADI trial. Diabetology and Metabolic Syndrome. ISSN 1758-5996. 11:109, p. 1–9. doi: 10.1186/s13098-019-0508-9. Full text in Research Archive
• Kalstad, Are; Tveit, Sjur Hansen; Myhre, Peder Langeland; Laake, Kristian; Opstad, Trine Baur & Tveit, Arnljot [Show all 10 contributors for this article] (2019). Leukocyte telomere length and serum polyunsaturated fatty acids, dietary habits, cardiovascular risk factors and features of myocardial infarction in elderly patients. BMC Geriatrics. ISSN 1471-2318. 19:376, p. 1–9. doi: 10.1186/s12877-019-1383-9. Full text in Research Archive
• Helseth, Ragnhild; Knudsen, Eva Cecilie; Eritsland, Jan; Opstad, Trine Baur; Arnesen, Harald & Andersen, Geir Øystein [Show all 7 contributors for this article] (2019). Glucose associated NETosis in patients with ST-elevation myocardial infarction: An observational study. BMC Cardiovascular Disorders. ISSN 1471-2261. 19:221, p. 1–8. doi: 10.1186/s12872-019-1205-1. Full text in Research Archive
• Helseth, Ragnhild; Shetelig, Christian; Andersen, Geir Øystein; Langseth, Miriam Sjåstad; Limalanathan, Shanmuganathan & Opstad, Trine Baur [Show all 10 contributors for this article] (2019). Neutrophil extracellular trap components associate with infarct size, ventricular function, and clinical outcome in STEMI. Mediators of Inflammation. ISSN 0962-9351. 2019:784691, p. 1–10. doi: 10.1155/2019/7816491. Full text in Research Archive
• Zaidi, Hani; Byrkjeland, Rune; Unhammer Njerve, Ida; Åkra, Sissel; Solheim, Svein & Arnesen, Harald [Show all 8 contributors for this article] (2019). Effects of exercise training on inflammasome-related mediators and their associations to glucometabolic variables in patients with combined coronary artery disease and type 2 diabetes mellitus: Sub-study of a randomized control trial. Diabetes & vascular disease research. ISSN 1479-1641. doi: 10.1177/1479164119836922. Full text in Research Archive
• Opstad, Trine Baur; Kalstad, Are Annesønn; Pettersen, Alf Åge Reistad; Arnesen, Harald & Seljeflot, Ingebjørg (2019). Novel biomolecules of ageing, sex differences and potential underlying mechanisms of telomere shortening in coronary artery disease. Experimental Gerontology. ISSN 0531-5565. 119, p. 53–60. doi: 10.1016/j.exger.2019.01.020. Full text in Research Archive
• Åkra, Sissel; Aksnes, Tonje Amb; Flaa, Arnljot; Eggesbø, Heidi Beate; Opstad, Trine Baur & Unhammer Njerve, Ida [Show all 7 contributors for this article] (2018). Interleukin-18 and the NLR family pyrin domain containing-3 inflammasome in adipose tissue are strongly associated with glucometabolic variables in a cohort of middle-aged men. Diabetes & vascular disease research. ISSN 1479-1641. 15(5), p. 458–464. doi: 10.1177/1479164118785307.
• Langseth, Miriam Sjåstad; Opstad, Trine Baur; Bratset, Vibeke; Solheim, Svein; Arnesen, Harald & Pettersen, Alf Åge Reistad [Show all 8 contributors for this article] (2018). Markers of neutrophil extracellular traps are associated with adverse clinical outcome in stable coronary artery disease. European Journal of Preventive Cardiology (EJPC). ISSN 2047-4873. 25(7), p. 762–769. doi: 10.1177/2047487318760618.
• Opstad, Trine Baur; Seljeflot, Ingebjørg; Bøhmer, Ellen; Arnesen, Harald & Halvorsen, Sigrun (2018). MMP-9 and its regulators TIMP-1 and EMMPRIN in patients with acute ST-elevation myocardial infarction: a NORDISTEMI substudy. Cardiology. ISSN 0008-6312. 139(1), p. 17–24. doi: 10.1159/000481684.
• Opstad, Trine Baur; Brusletto, Berit Sletbakk; Arnesen, Harald; Pettersen, Alf Åge Reistad & Seljeflot, Ingebjørg (2017). Cigarette smoking represses expression of cytokine IL-12 and its regulator miR-21-An observational study in patients with coronary artery disease. Immunobiology. ISSN 0171-2985. 222(2), p. 169–175. doi: 10.1016/j.imbio.2016.10.007. Full text in Research Archive
• Helseth, Ragnhild; Solheim, Svein; Arnesen, Harald; Seljeflot, Ingebjørg & Opstad, Trine Baur (2016). The time course of markers of neutrophil extracellular traps in patients undergoing revascularisation for acute myocardial infarction or stable angina pectoris. Mediators of Inflammation. ISSN 0962-9351. 2016. doi: 10.1155/2016/2182358.
• Opstad, Trine Baur; Arnesen, Harald; Pettersen, Alf Åge Reistad & Seljeflot, Ingebjørg (2016). Combined Elevated Levels of the Proinflammatory Cytokines IL-18 and IL-12 Are Associated with Clinical Events in Patients with Coronary Artery Disease: An Observational Study. Metabolic Syndrome and Related Disorders. ISSN 1540-4196. 14(5), p. 242–248. doi: 10.1089/met.2015.0130.
• Helseth, Ragnhild; Weiss, Thomas; Opstad, Trine Baur; Siegbahn, Agneta; Solheim, Svein & Freynhofer, Matthias K [Show all 9 contributors for this article] (2015). Associations between circulating proteins and corresponding genes expressed in coronary thrombi in patients with acute myocardial infarction. Thrombosis Research. ISSN 0049-3848. 136(6), p. 1240–1244. doi: 10.1016/j.thromres.2015.10.005. Show summary

  Introduction Several genes are expressed in aspirated coronary thrombi in acute myocardial infarction (AMI), exhibiting dynamic changes along ischemic time. Whether soluble biomarkers reflect the local gene environment and ischemic time is unclear. We explored whether circulating biomarkers were associated with corresponding coronary thrombi genes and total ischemic time. Material and methods In 33 AMI patients undergoing percutaneous coronary intervention (PCI), blood samples were collected within 6–24 h for markers related to plaque rupture (metalloproteinase 9, tissue inhibitor of metalloproteinases 1), platelet and endothelial cell activation (P-selectin, CD40 ligand, PAR-1), hemostasis (tissue factor, tissue plasminogen activator, plasminogen activator inhibitor 1, free and total tissue factor pathway inhibitor, D-dimer, prothrombin fragment 1 + 2), inflammation (interleukin 8 and 18, fractalkine, monocyte chemoattractant protein 1 (MCP-1), CXCL1, pentraxin 3, myeloperoxidase) and galectin 3, caspase 8 and epidermal growth factor (EGF). Laboratory analyses were performed by Proximity Extension Assay (Proseek Multiplex CVD I96 × 96), ELISAs and RT-PCR. Results Only circulating P-selectin correlated to the corresponding P-selectin gene expression in thrombi (r = 0.530, p = 0.002). Plasma galectin 3, fractalkine, MCP-1 and caspase 8 correlated inversely to ischemic time (r = − 0.38–0.50, all p < 0.05), while plasma MCP-1, galectin 3 and EGF were higher at short (≤ 4 h) vs. long (> 4 h) ischemic time (all p < 0.05). Conclusions The dynamic changes in circulating mediators along ischemic time were not reflected in the profile of locally expressed genes. These observations indicate a locally confined milieu within the site of atherothrombosis, which may be important for selective therapy.

• Helseth, Ragnhild Merckoll; Seljeflot, Ingebjørg; Opstad, Trine Baur; Solheim, Svein; Freynhofer, M & Arnesen, Harald [Show all 8 contributors for this article] (2015). Genes expressed in coronary thrombi are associated with ischemic time in patients with acute myocardial infarction. Thrombosis Research. ISSN 0049-3848. 135(2), p. 329–333. doi: 10.1016/j.thromres.2014.11.028.
• Furenes, Eline Bredal; Opstad, Trine Baur; Solheim, Svein; Lunde, Ketil; Arnesen, Harald & Seljeflot, Ingebjørg (2015). The Influence of Autologous Bone Marrow Stem Cell Transplantation on Matrix Metalloproteinases in Patients Treated for Acute ST-Elevation Myocardial Infarction. Mediators of Inflammation. ISSN 0962-9351. 2014. doi: 10.1155/2014/385901.
• Opstad, Trine Baur; Arnesen, Harald; Pettersen, Alf Åge Reistad & Seljeflot, Ingebjørg (2014). The MMP-9 -1562 C/T polymorphism in the presence of metabolic syndrome increases the risk of clinical events in patients with coronary artery disease. PLOS ONE. ISSN 1932-6203. 9(9:e106816). doi: 10.1371/journal.pone.0106816.
• Helseth, Ragnhild Merckoll; Solheim, Svein; Opstad, Trine Baur; HOFFMANN, PAVEL; Arnesen, Harald & Seljeflot, Ingebjørg (2014). The time profile of pentraxin 3 in patients with acute ST-elevation myocardial infarction and stable angina pectoris undergoing percutaneous coronary intervention. Mediators of Inflammation. ISSN 0962-9351. 2014. doi: 10.1155/2014/608414.
• Opstad, Trine Baur; Pettersen, Alf Åge Reistad; Arnesen, Harald & Seljeflot, Ingebjørg (2013). The co-existence of the IL-18+183 A/G and MMP-9 -1562 C/T polymorphism predicts future clinical events in stable coronary artery disease patients. PLOS ONE. ISSN 1932-6203. 8(9). doi: 10.1371/journal.pone.0074498.
• Pettersen, Alf Åge Reistad; Opstad, Trine Baur; Arnesen, Harald; Seljeflot, Ingebjørg & Bratseth, Vibeke (2012). Markers of hypercoagulability in CAD patients. Effects of single aspirin and clopidogrel treatment. Thrombosis Journal. ISSN 1477-9560. 10. doi: 10.1186/1477-9560-10-12.
• Unhammer Njerve, Ida; Pettersen, Alf Åge Reistad; Opstad, Trine Baur; Arnesen, Harald & Seljeflot, Ingebjørg (2012). Fractalkine and Its Receptor (CX3CR1) in Patients with Stable Coronary Artery Disease and Diabetes Mellitus. Metabolic Syndrome and Related Disorders. ISSN 1540-4196. 10(6), p. 400–406. doi: 10.1089/met.2012.0052.
• Pettersen, Alf Åge Reistad; Arnesen, Harald; Opstad, Trine Baur; Bratseth, Vibeke & Seljeflot, Ingebjørg (2012). Markers of endothelial and platelet activation are associated with high on-aspirin platelet reactivity in patients with stable coronary artery disease. Thrombosis Research. ISSN 0049-3848. 130(3), p. 424–428. doi: 10.1016/j.thromres.2012.06.016.
• Opstad, Trine Baur; Pettersen, Alf Åge Reistad; Weiss, Thomas; Åkra, Sissel; Øvstebø, Reidun & Arnesen, Harald [Show all 7 contributors for this article] (2012). Genetic variation, gene-expression and circulating levels of matrix metalloproteinase-9 in patients with stable coronary artery disease. Clinica Chimica Acta. ISSN 0009-8981. 413(1-2), p. 113–120. doi: 10.1016/j.cca.2011.09.004.
• Opstad, Trine Baur; Pettersen, Alf Åge Reistad; Arnesen, Harald & Seljeflot, Ingebjørg (2011). Circulating levels of IL-18 are significantly influenced by the IL-18+183 A/G Polymorphism in Coronary Artery Disease patients with Diabetes Type 2 and the Metabolic Syndrome: An Observational Study. Cardiovascular Diabetology. ISSN 1475-2840. 10. doi: 10.1186/1475-2840-10-110.
• Pettersen, Alf-Åge; Arnesen, Harald; Opstad, Trine Baur & Seljeflot, Ingebjørg (2011). The influence of CYP 2C19*2 polymorphism on platelet function testing during single antiplatelet treatment with clopidogrel. Thrombosis Journal. ISSN 1477-9560. 9. doi: 10.1186/1477-9560-9-4.
• Opstad, Trine Baur; Eilertsen, Anette Løken; Høibraaten, Else; Skretting, Grethe & Sandset, Per Morten (2010). Tissue factor pathway inhibitor polymorphisms in women with and without a history of venous thrombosis and the effects of postmenopausal hormone therapy. Blood Coagulation and Fibrinolysis. ISSN 0957-5235. 21(6), p. 516–521. doi: 10.1097/MBC.0b013e32833a06cd.
• Opstad, Trine Baur; Pettersen, Alf Åge Reistad; Weiss, Thomas; Arnesen, Harald & Seljeflot, Ingebjørg (2010). Gender differences of polymorphisms in the TF and TFPI genes, as related to pheotypes in patients with coronary heart disease and type-2 diabetes. Thrombosis Journal. ISSN 1477-9560. 8. doi: 10.1186/1477-9560-8-7.

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• Unhammer Njerve, Ida; Pettersen, Alf Åge Reistad; Opstad, Trine Baur; Bratseth, Vibeke; Arnesen, Harald & Seljeflot, Ingebjørg (2012). Fractalkine (CX3CL1) in patients with stable coronary artery disease (CAD) with and without diabetes mellitus. Cardiovascular Research (CVR). ISSN 0008-6363. 93, p. S78–S79.
• Pettersen, Alf Åge Reistad; Arnesen, Harald; Opstad, Trine Baur; Bratseth, Vibeke & Seljeflot, Ingebjørg (2012). Markers of endothelial and platelet activation are associated with high on-aspirin residual platelet reactivity. European Heart Journal. ISSN 0195-668X. 33(Supp. 1), p. 306–307.
• Opstad, Trine Baur; Pettersen, A A; Åkra, Sissel; Arnesen, H & Seljeflot, Ingebjørg (2012). The influence of the IL-18+183 A/G polymorphism on gene- and protein expression in stable coronary artery disease patients. Cardiovascular Research (CVR). ISSN 0008-6363. 93, p. S36–S36.
• Opstad, Trine Baur; Pettersen, Alf Åge Reistad; Åkra, Sissel; Weiss, Thomas; Arnesen, Harald & Seljeflot, Ingebjørg (2010). TF and TFPI polymorphisms in coronary heart disease and diabetes type 2. Atherosclerosis Supplements. ISSN 1567-5688. 11(2), p. 146–147.
• Pettersen, Alf Åge Reistad; Arnesen, Harald; Opstad, Trine Baur; Åkra, Sissel & Seljeflot, Ingebjørg (2010). The influence of cyp 2c19*2 polymorphism on functional platelet testing in clopidogrel users. European Heart Journal. ISSN 0195-668X. 31, p. 969–969.

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