Therese Sørlie

• Ohnstad, Hege Oma; Blix, Egil Støre; Akslen, Lars Andreas; Gilje, Bjørnar; Raj, Sunil Xavier & Skjerven, Helle [Show all 46 contributors for this article] (2024). Impact of Prosigna test on adjuvant treatment decision in lymph node-negative early breast cancer—a prospective national multicentre study (EMIT-1). ESMO Open. ISSN 2059-7029. 9(6). doi: 10.1016/j.esmoop.2024.103475.
• Polec, Anna; Ekstrøm, Per Olaf; Fougner, Christian; Sørlie, Therese & Norum, Jens Henrik (2023). Rapid assessment of 3-dimensional intra-tumor heterogeneity through cycling temperature capillary electrophoresis. BMC Research Notes. ISSN 1756-0500. 16. doi: 10.1186/s13104-023-06437-5. Full text in Research Archive
• van Amerongen, Renée; Bentires-Alj, Mohamed; van Boxtel, Antonius L.; Clarke, Robert B.; Fre, Silvia & Suarez, Eva Gonzalez [Show all 13 contributors for this article] (2023). Imagine beyond: recent breakthroughs and next challenges in mammary gland biology and breast cancer research. Journal of mammary gland biology and neoplasia. ISSN 1083-3021. 28(1). doi: 10.1007/s10911-023-09544-y. Full text in Research Archive
• Haugen, Mads; von der Lippe Gythfeldt, Hedda; Egeland, Eivind Valen; Normann, Lisa Svartdal; Pandya, Abhilash D. & Vedin, Lise-Lotte [Show all 15 contributors for this article] (2023). Liver X receptors induce antiproliferative effects in basal-like breast cancer. Molecular Oncology. ISSN 1574-7891. doi: 10.1002/1878-0261.13476. Full text in Research Archive
• Figlioli, Gisella; Billaud, Amandine; Ahearn, Thomas U.; Antonenkova, Natalia N.; Becher, Heiko & Beckmann, Matthias W. [Show all 114 contributors for this article] (2023). FANCM missense variants and breast cancer risk: a case-control association study of 75,156 European women. European Journal of Human Genetics. ISSN 1018-4813. 31(5), p. 578–587. doi: 10.1038/s41431-022-01257-w. Full text in Research Archive Show summary

  Evidence from literature, including the BRIDGES study, indicates that germline protein truncating variants (PTVs) in FANCM confer moderately increased risk of ER-negative and triple-negative breast cancer (TNBC), especially for women with a family history of the disease. Association between FANCM missense variants (MVs) and breast cancer risk has been postulated. In this study, we further used the BRIDGES study to test 689 FANCM MVs for association with breast cancer risk, overall and in ER-negative and TNBC subtypes, in 39,885 cases (7566 selected for family history) and 35,271 controls of European ancestry. Sixteen common MVs were tested individually; the remaining rare 673 MVs were tested by burden analyses considering their position and pathogenicity score. We also conducted a meta-analysis of our results and those from published studies. We did not find evidence for association for any of the 16 variants individually tested. The rare MVs were significantly associated with increased risk of ER-negative breast cancer by burden analysis comparing familial cases to controls (OR = 1.48; 95% CI 1.07–2.04; P = 0.017). Higher ORs were found for the subgroup of MVs located in functional domains or predicted to be pathogenic. The meta-analysis indicated that FANCM MVs overall are associated with breast cancer risk (OR = 1.22; 95% CI 1.08–1.38; P = 0.002). Our results support the definition from previous analyses of FANCM as a moderate-risk breast cancer gene and provide evidence that FANCM MVs could be low/moderate risk factors for ER-negative and TNBC subtypes. Further genetic and functional analyses are necessary to clarify better the increased risks due to FANCM MVs

• Langille, Ellen; Al-Zahrani, Khalid N.; Ma, Zhibo; Liang, Minggao; Uuskula-Reimand, Liis & Espin, Roderic [Show all 36 contributors for this article] (2022). Loss of Epigenetic Regulation Disrupts Lineage Integrity, Induces Aberrant Alveogenesis, and Promotes Breast Cancer. Cancer Discovery. ISSN 2159-8274. 12(12), p. 2930–2953. doi: 10.1158/2159-8290.CD-21-0865.
• Staaf, Johan; Häkkinen, Jari; Hegardt, Cecilia; Saal, Lao H.; Kimbung, Siker & Hedenfalk, Ingrid [Show all 25 contributors for this article] (2022). RNA sequencing-based single sample predictors of molecular subtype and risk of recurrence for clinical assessment of early-stage breast cancer. NPJ breast cancer. ISSN 2374-4677. 8:94(1), p. 1–17. doi: 10.1038/s41523-022-00465-3. Full text in Research Archive
• Bergholtz, Helga; Lien, Tonje Gulbrandsen; Lingaas, Frode & Sørlie, Therese (2022). Comparative analysis of the molecular subtype landscape in canine and human mammary gland tumors. Journal of mammary gland biology and neoplasia. ISSN 1083-3021. p. 171–183. doi: 10.1007/s10911-022-09523-9. Full text in Research Archive
• Bjørklund, Sunniva; Aure, Miriam Ragle; Häkkinen, Jari; Vallon-Christersson, Johan; Kumar, Surendra & Bull Evensen, Katrine [Show all 34 contributors for this article] (2022). Subtype and cell type specific expression of lncRNAs provide insight into breast cancer. Communications Biology. ISSN 2399-3642. 5(1), p. 1–14. doi: 10.1038/s42003-022-03559-7. Full text in Research Archive
• Bergholtz, Helga; Carter, Jodi M.; Cesano, Alessandra; Cheang, Maggie Chon U.; Church, Sarah E. & Divakar, Prajan [Show all 32 contributors for this article] (2021). Best practices for spatial profiling for breast cancer research with the GeoMx® digital spatial profiler. Cancers. ISSN 2072-6694. 13(17). doi: 10.3390/cancers13174456.
• Rye, Inga Hansine; Huse, Kanutte; Josefsson, Sarah Elisabet; Kildal, Wanja; Danielsen, Håvard Emil Greger & Schlichting, Ellen [Show all 28 contributors for this article] (2021). Breast cancer metastasis: immune profiling of lymph nodes reveals exhaustion of effector T cells and immunosuppression. Molecular Oncology. ISSN 1574-7891. 16(1), p. 88–103. doi: 10.1002/1878-0261.13047. Full text in Research Archive
• Lien, Tonje Gulbrandsen; Ohnstad, Hege Oma; Lingjærde, Ole Christian; Vallon-Christersson, Johan; Aaserud, Marit & Sveli, My Anh Tu [Show all 12 contributors for this article] (2021). Sample preparation approach influences pam50 risk of recurrence score in early breast cancer. Cancers. ISSN 2072-6694. 13(23). doi: 10.3390/cancers13236118. Full text in Research Archive
• Xu, Haifeng; Lillebergen, Tonje Lien; Bergholtz, Helga; Fleischer, Thomas; Djerroudi, Lounes & Vincent-Salomon, Anne [Show all 8 contributors for this article] (2021). Multi-omics marker analysis enables early prediction of breast tumor progression. Frontiers in Genetics. ISSN 1664-8021. 12, p. 1–14. doi: 10.3389/fgene.2021.670749. Full text in Research Archive
• Lilleborge, Marie; Falk, Ragnhild Sørum; Sørlie, Therese; Ursin, Giske & Hofvind, Solveig (2021). Can breast cancer be stopped? Modifiable risk factors of breast cancer among women with a prior benign or premalignant lesion. International Journal of Cancer. ISSN 0020-7136. 149(6), p. 1247–1256. doi: 10.1002/ijc.33680. Full text in Research Archive
• Liu, Jingjing; van der Smissen, Wendy J. C. Prager; Collee, Margriet J.; Bolla, Manjeet K.; Wang, Qin & Kristensen, Vessela N. [Show all 82 contributors for this article] (2020). Germline HOXB13 mutations p.G84E and p.R217C do not confer an increased breast cancer risk. Scientific Reports. ISSN 2045-2322. 10:9688(1), p. 1–14. doi: 10.1038/s41598-020-65665-y. Full text in Research Archive Show summary

  In breast cancer, high levels of homeobox protein Hox-B13 (HOXB13) have been associated with disease progression of ER-positive breast cancer patients and resistance to tamoxifen treatment. Since HOXB13 p.G84E is a prostate cancer risk allele, we evaluated the association between HOXB13 germline mutations and breast cancer risk in a previous study consisting of 3,270 familial non-BRCA1/2 breast cancer cases and 2,327 controls from the Netherlands. Although both recurrent HOXB13 mutations p.G84E and p.R217C were not associated with breast cancer risk, the risk estimation for p.R217C was not very precise. To provide more conclusive evidence regarding the role of HOXB13 in breast cancer susceptibility, we here evaluated the association between HOXB13 mutations and increased breast cancer risk within 81 studies of the international Breast Cancer Association Consortium containing 68,521 invasive breast cancer patients and 54,865 controls. Both HOXB13 p.G84E and p.R217C did not associate with the development of breast cancer in European women, neither in the overall analysis (OR=1.035, 95% CI=0.859–1.246, P=0.718 and OR=0.798, 95% CI=0.482–1.322, P=0.381 respectively), nor in specifc high-risk subgroups or breast cancer subtypes. Thus, although involved in breast cancer progression, HOXB13 is not a material breast cancer susceptibility gene.

• Bergholtz, Helga; Kumar, Surendra; Wärnberg, Fredrik; Luders, Torben; Kristensen, Vessela N. & Sørlie, Therese (2020). Comparable cancer-relevant mutation profiles in synchronous ductal carcinoma in situ and invasive breast cancer. Cancer Reports. ISSN 2573-8348. 3:e1248(3), p. 1–7. doi: 10.1002/cnr2.1248. Full text in Research Archive
• Fougner, Christian; Bergholtz, Helga; Norum, Jens Henrik & Sørlie, Therese (2020). Re-definition of claudin-low as a breast cancer phenotype. Nature Communications. ISSN 2041-1723. 11(1), p. 1–11. doi: 10.1038/s41467-020-15574-5. Full text in Research Archive
• Engelsen, Agnete ; Wnuk-Lipinska, Katarzyna; Bougnaud, Sébastien; Vatter, Fanny Pelissier; Tiron, Crina Elena & Villadsen, René [Show all 28 contributors for this article] (2020). AXL is a driver of stemness in normal mammary gland and breast cancer. iScience. ISSN 2589-0042. 23, p. 1–40. doi: 10.1016/j.isci.2020.101649.
• Bergholtz, Helga; Lien, Tonje Gulbrandsen; Swanson, David; Frigessi Di Rattalma, Arnoldo; Daidone, Maria Grazia & Tost, Jörg [Show all 8 contributors for this article] (2020). Contrasting DCIS and invasive breast cancer by subtype suggests basal-like DCIS as distinct lesions. NPJ breast cancer. ISSN 2374-4677. 6. doi: 10.1038/s41523-020-0167-x. Full text in Research Archive
• Knutsen, Erik; Lellahi, Seyed Mohammad; Aure, Miriam Ragle; Nord, Silje; Fismen, Silje & Larsen, Kenneth Bowitz [Show all 14 contributors for this article] (2020). The expression of the long NEAT1_2 isoform is associated with human epidermal growth factor receptor 2-positive breast cancers. Scientific Reports. ISSN 2045-2322. 10:1277, p. 1–14. doi: 10.1038/s41598-020-57759-4. Full text in Research Archive
• Tekpli, Xavier; Lien, Tonje Gulbrandsen; Røssevold, Andreas Hagen; Nebdal, Daniel J.H.; Borgen, Elin & Ohnstad, Hege Oma [Show all 19 contributors for this article] (2019). An independent poor-prognosis subtype of breast cancer defined by a distinct tumor immune microenvironment. Nature Communications. ISSN 2041-1723. 10:5499, p. 1–14. doi: 10.1038/s41467-019-13329-5. Full text in Research Archive
• Fougner, Christian; Bergholtz, Helga; Kuiper, Raoul; Norum, Jens Henrik & Sørlie, Therese (2019). Claudin-low-like mouse mammary tumors show distinct transcriptomic patterns uncoupled from genomic drivers. Breast Cancer Research. ISSN 1465-5411. 21(1), p. 1–13. doi: 10.1186/s13058-019-1170-8. Full text in Research Archive
• Norum, Jens Henrik; Frings, Oliver; Kasper, Maria; Bergholtz, Helga; Thime, Helene Zell & Bergström, Åsa [Show all 11 contributors for this article] (2019). GLI1-induced mammary gland tumours are transplantable and maintain major molecular features. International Journal of Cancer. ISSN 0020-7136. doi: 10.1002/ijc.32522.
• Swanson, David; Lien, Tonje Gulbrandsen; Bergholtz, Helga; Sørlie, Therese & Frigessi Di Rattalma, Arnoldo (2019). A Bayesian two-way latent structure model for genomic data integration reveals few pan-genomic cluster subtypes in a breast cancer cohort. Bioinformatics. ISSN 1367-4803. 35(23), p. 4886–4897. doi: 10.1093/bioinformatics/btz381. Show summary

  Motivation Unsupervised clustering is important in disease subtyping, among having other genomic applications. As genomic data has become more multifaceted, how to cluster across data sources for more precise subtyping is an ever more important area of research. Many of the methods proposed so far, including iCluster and Cluster of Cluster Assignments (COCAs), make an unreasonable assumption of a common clustering across all data sources, and those that do not are fewer and tend to be computationally intensive. Results We propose a Bayesian parametric model for integrative, unsupervised clustering across data sources. In our two-way latent structure model, samples are clustered in relation to each specific data source, distinguishing it from methods like COCAs and iCluster, but cluster labels have across-dataset meaning, allowing cluster information to be shared between data sources. A common scaling across data sources is not required, and inference is obtained by a Gibbs Sampler, which we improve with a warm start strategy and modified density functions to robustify and speed convergence. Posterior interpretation allows for inference on common clusterings occurring among subsets of data sources. An interesting statistical formulation of the model results in sampling from closed-form posteriors despite incorporation of a complex latent structure. We fit the model with Gaussian and more general densities, which influences the degree of across-dataset cluster label sharing. Uniquely among integrative clustering models, our formulation makes no nestedness assumptions of samples across data sources so that a sample missing data from one genomic source can be clustered according to its existing data sources. We apply our model to a Norwegian breast cancer cohort of ductal carcinoma in situ and invasive tumors, comprised of somatic copy-number alteration, methylation and expression datasets. We find enrichment in the Her2 subtype and ductal carcinoma among those observations exhibiting greater cluster correspondence across expression and CNA data. In general, there are few pan-genomic clusterings, suggesting that models assuming a common clustering across genomic data sources might yield misleading results. Availability and implementation The model is implemented in an R package called twl (‘two-way latent’), available on CRAN. Data for analysis are available within the R package.

• Bergholtz, Helga; Lien, Tonje Gulbrandsen; Ursin, Giske; Holmen, Marit Muri; Helland, Åslaug & Sørlie, Therese [Show all 7 contributors for this article] (2019). A Longitudinal Study of the Association between Mammographic Density and Gene Expression in Normal Breast Tissue. Journal of mammary gland biology and neoplasia. ISSN 1083-3021. 24(2), p. 163–175. doi: 10.1007/s10911-018-09423-x. Full text in Research Archive
• Norum, Jens Henrik; Skarpen, Ellen; Brech, Andreas; Kuiper, Raoul; Waaler, Jo & Krauss, Stefan [Show all 7 contributors for this article] (2018). The tankyrase inhibitor G007-LK inhibits small intestine LGR5+ stem cell proliferation without altering tissue morphology. Biological Research (Print). ISSN 0716-9760. 51(1). doi: 10.1186/s40659-017-0151-6. Full text in Research Archive
• Laurberg, Tinne; Tramm, Trine; Nielsen, Torsten; Alsner, Jan; Nord, Silje & Myhre, Simen [Show all 15 contributors for this article] (2018). Intrinsic subtypes and benefit from postmastectomy radiotherapy in node-positive premenopausal breast cancer patients who received adjuvant chemotherapy - results from two independent randomized trials. Acta Oncologica. ISSN 0284-186X. 57(1), p. 38–43. doi: 10.1080/0284186X.2017.1401735. Show summary

  BACKGROUND: The study of the intrinsic molecular subtypes of breast cancer has revealed differences among them in terms of prognosis and response to chemotherapy and endocrine therapy. However, the ability of intrinsic subtypes to predict benefit from adjuvant radiotherapy has only been examined in few studies. METHODS: Gene expression-based intrinsic subtyping was performed in 228 breast tumors collected from two independent post-mastectomy clinical trials (British Columbia and the Danish Breast Cancer Cooperative Group 82b trials), where pre-menopausal patients with node-positive disease were randomized to adjuvant radiotherapy or not. All patients received adjuvant chemotherapy and a subgroup of patients underwent ovarian ablation. Tumors were classified into intrinsic subtypes: Luminal A, Luminal B, HER2-enriched, Basal-like and Normal-like using the research-based PAM50 classifier. RESULTS: In the British Columbia study, patients treated with radiation had an overall significant lower incidence of locoregional recurrence compared to the controls. For Luminal A tumors the risk of loco-regional recurrence was low and was further lowered by adjuvant radiation. These findings were validated in the DBCG 82b study. The individual data from the two cohorts were merged, the hazard ratio (HR) for loco-regional recurrence associated with giving radiation was 0.34 (0.19 to 0.61) overall and 0.12 (0.03 to 0.52) for Luminal A tumors. CONCLUSIONS: In both postmastectomy trials, patients with Luminal A tumors turned out to have a significant lower incidence of loco-regional recurrence when randomized to adjuvant radiotherapy, leaving no indication to omit postmastectomy adjuvant radiation in pre-menopausal high-risk patients with Luminal A tumors. It was not possible to evaluate the effect of radiotherapy among the other subtypes because of limited sample sizes.

• Gil Del Alcazar, Carlos Rodrigo; Huh, Sung Jin; Ekram, Muhammad B.; Trinh, Anne; Liu, Lin L. & Beca, Francisco [Show all 30 contributors for this article] (2017). Immune escape in breast cancer during in situ to invasive carcinoma transition. Cancer Discovery. ISSN 2159-8274. 7(10), p. 1098–1115. doi: 10.1158/2159-8290.CD-17-0222.
• Ohnstad, Hege Oma; Borgen, Elin; Falk, Ragnhild Sørum; Lien, Tonje Gulbrandsen; Aaserud, Marit & Sveli, My Anh Tu [Show all 14 contributors for this article] (2017). Prognostic value of PAM50 and risk of recurrence score in patients with early-stage breast cancer with long-term follow-up. Breast Cancer Research. ISSN 1465-5411. 19(120). doi: 10.1186/s13058-017-0911-9. Full text in Research Archive
• Sas-Chen, Aldema; Aure, Miriam Ragle; Leibovich, Limor; Carvalho, Silvia; Enuka, Yehoshua & Körner, Cindy [Show all 38 contributors for this article] (2016). LIMT is a novel metastasis inhibiting lncRNA suppressed by EGF and downregulated in aggressive breast cancer. EMBO Molecular Medicine. ISSN 1757-4676. 8(9), p. 1052–1064. doi: 10.15252/emmm.201606198. Full text in Research Archive
• Lesurf, Robert; Aure, Miriam Ragle; Mørk, Hanne Håberg; Vitelli, Valeria; Lundgren, Steinar & Børresen-Dale, Anne-Lise [Show all 10 contributors for this article] (2016). Molecular Features of Subtype-Specific Progression from Ductal Carcinoma In Situ to Invasive Breast Cancer. Cell reports. ISSN 2211-1247. 16(4), p. 1166–1179. doi: 10.1016/j.celrep.2016.06.051. Full text in Research Archive
• Skrbo, Nirma; Tenstad, Ellen; Mælandsmo, Gunhild; Sørlie, Therese & Andersen, Kristin (2015). From autonomy to community; new perspectives on tumorigenicity and therapy resistance. Cancer Treatment Reviews. ISSN 0305-7372. 41(10), p. 809–813. doi: 10.1016/j.ctrv.2015.10.004.
• Borgquist, Signe; Zhou, Wenjing; Jirström, Karin; Amini, Rose-Marie; Sollie, Thomas & Sørlie, Therese [Show all 9 contributors for this article] (2015). The prognostic role of HER2 expression in ductal breast carcinoma in situ (DCIS); a population-based cohort study. BMC Cancer. ISSN 1471-2407. 15:468. doi: 10.1186/s12885-015-1479-3.
• Norum, Jens Henrik; Bergström, Åsa; Andersson, Agneta Birgitta; Kuiper, Raoul V.; Hoelzl, Maria A. & Sørlie, Therese [Show all 7 contributors for this article] (2015). A conditional transgenic mouse line for targeted expression of the stem cell marker LGR5. Developmental Biology. ISSN 0012-1606. 404(2), p. 35–48. doi: 10.1016/j.ydbio.2015.05.002.
• Potapenko, Ivan Olegovich; Luders, Torben; Russnes, Hege Elisabeth Giercksky; Helland, Åslaug; Sørlie, Therese & Kristensen, Vessela N. [Show all 10 contributors for this article] (2015). Glycan-related gene expression signatures in breast cancer subtypes; relation to survival. Molecular Oncology. ISSN 1574-7891. 9(4), p. 861–876. doi: 10.1016/j.molonc.2014.12.013.
• Skrbo, Nirma; Hjortland, Geir Olav; Kristian, Alexandr; Holm, Ruth; Nordgard, Silje H. & Prasmickaite, Lina [Show all 10 contributors for this article] (2014). Differential in vivo tumorigenicity of distinct subpopulations from a luminal-like breast cancer xenograft. PLOS ONE. ISSN 1932-6203. 9(11). doi: 10.1371/journal.pone.0113278. Full text in Research Archive
• Tramm, Trine; Kyndi, Marianne; Myhre, Simen; Nordgard, Silje H.; Alsner, Jan & Sørensen, Flemming Brandt [Show all 8 contributors for this article] (2014). Relationship between the prognostic and predictive value of the intrinsic subtypes and a validated gene profile predictive of loco-regional control and benefit from post-mastectomy radiotherapy in patients with high-risk breast cancer. Acta Oncologica. ISSN 0284-186X. 53(10), p. 1337–1346. doi: 10.3109/0284186X.2014.925580.
• Tramm, Trine; Mohammed, Hayat; Myhre, Simen; Kyndi, Marianne; Alsner, Jan & Børresen-Dale, Anne-Lise [Show all 9 contributors for this article] (2014). Development and Validation of a Gene Profile Predicting Benefit of Postmastectomy Radiotherapy in Patients with High-Risk Breast Cancer: A Study of Gene Expression in the DBCG82bc Cohort. Clinical Cancer Research. ISSN 1078-0432. 20(20), p. 5272–5280. doi: 10.1158/1078-0432.CCR-14-0458. Full text in Research Archive Show summary

  Purpose: To identify genes predicting benefit of radiotherapy in patients with high-risk breast cancer treated with systemic therapy and randomized to receive or not receive postmastectomy radiotherapy (PMRT). Experimental Design: The study was based on the Danish Breast Cancer Cooperative Group (DBCG82bc) cohort. Gene-expression analysis was performed in a training set of frozen tumor tissue from 191 patients. Genes were identified through the Lasso method with the endpoint being locoregional recurrence (LRR). A weighted gene-expression index (DBCG-RT profile) was calculated and transferred to quantitative real-time PCR (qRT-PCR) in corresponding formalin-fixed, paraffin-embedded (FFPE) samples, before validation in FFPE from 112 additional patients. Results: Seven genes were identified, and the derived DBCG-RT profile divided the 191 patients into “high LRR risk” and “low LRR risk” groups. PMRT significantly reduced risk of LRR in “high LRR risk” patients, whereas “low LRR risk” patients showed no additional reduction in LRR rate. Technical transfer of the DBCG-RT profile to FFPE/qRT-PCR was successful, and the predictive impact was successfully validated in another 112 patients. Conclusions: A DBCG-RT gene profile was identified and validated, identifying patients with very low risk of LRR and no benefit from PMRT. The profile may provide a method to individualize treatment with PMRT

• Zhao, Xi; Rødland, Einar Andreas; Sørlie, Therese; Vollan, Hans Kristian Moen; Russnes, Hege Elisabeth Giercksky & Kristensen, Vessela N. [Show all 8 contributors for this article] (2014). Systematic assessment of prognostic gene signatures for breast cancer shows distinct influence of time and ER status. BMC Cancer. ISSN 1471-2407. 14(211). doi: 10.1186/1471-2407-14-211.
• Norum, Jan; Andersen, Kristin & Sørlie, Therese (2014). Lessons learned from the intrinsic subtypes of breast cancer in the quest for precision therapy. British Journal of Surgery. ISSN 0007-1323. 101(8), p. 925–938. doi: 10.1002/bjs.9562.
• Grinde, Maria Tunset; Skrbo, Nirma; Moestue, Siver Andreas; Rødland, Einar Andreas; Borgan, Eldrid & Kristian, Alexandr [Show all 14 contributors for this article] (2014). Interplay of choline metabolites and genes in patient-derived breast cancer xenografts. Breast Cancer Research. ISSN 1465-5411. 16(1). doi: 10.1186/bcr3597. Full text in Research Archive
• Borgan, Eldrid; Lindholm, Evita Maria; Moestue, Siver Andreas; Mælandsmo, Gunhild; Lingjærde, Ole Christian & Gribbestad, Ingrid S [Show all 9 contributors for this article] (2013). Subtype-specific response to bevacizumab is reflected in the metabolome and transcriptome of breast cancer xenografts. Molecular Oncology. ISSN 1574-7891. 7(1), p. 130–142. doi: 10.1016/j.molonc.2012.10.005. Full text in Research Archive
• Zhou, Wenjing; Jirström, Karin; Amini, Rose-Marie; Fjällskog, Marie Louise; Sollie, Thomas & Lindman, Henrik [Show all 9 contributors for this article] (2013). Molecular subtypes in ductal carcinoma in situ of the breast and their relation to prognosis: a population-based cohort study. BMC Cancer. ISSN 1471-2407. 13:512. doi: 10.1186/1471-2407-13-512.
• Tramm, Trine; Hennig, Guido; Kyndi, Marianne; Alsner, Jan; Sørensen, Flemming & Myhre, Simen [Show all 8 contributors for this article] (2013). Reliable PCR quantitation of estrogen, progesterone and ERBB2 receptor mRNA from formalin-fixed, paraffin-embedded tissue is independent of prior macro-dissection. Virchows Archiv. ISSN 0945-6317. 463(6), p. 775–786. doi: 10.1007/s00428-013-1486-1.
• Myhre, Simen; Lingjærde, Ole Christian; Hennessy, Bryan T; Aure, Miriam Ragle; Carey, Mark S & Alsner, Jan [Show all 11 contributors for this article] (2013). Influence of DNA copy number and mRNA levels on the expression of breast cancer related proteins. Molecular Oncology. ISSN 1574-7891. 7(3), p. 704–718. doi: 10.1016/j.molonc.2013.02.018.
• Guiu, Séverine; Michiels, Stefan; Andre, Fabrice; Cortes, Javier; Denkert, Carsten & Di Leo, Angelo [Show all 14 contributors for this article] (2012). Molecular subclasses of breast cancer: how do we define them? The IMPAKT 2012 Working Group Statement. Annals of Oncology. ISSN 0923-7534. 23(12), p. 2997–3006. doi: 10.1093/annonc/mds586.
• Kim, Ji Young; Villadsen, René; Sørlie, Therese; Fogh, Louise; Gronlund, Signe Z & Fridriksdottir, Agla J. [Show all 15 contributors for this article] (2012). Tumor initiating but differentiated luminal-like breast cancer cells are highly invasive in the absence of basal-like activity. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 109(16), p. 6124–6129. doi: 10.1073/pnas.1203203109.
• Kristensen, Vessela N.; Vaske, Charles J.; Ursini-Siegel, Josie; Van Loo, Peter; Nordgard, Silje H. & Sachidanandam, Ravi [Show all 15 contributors for this article] (2012). Integrated molecular profiles of invasive breast tumors and ductal carcinoma in situ (DCIS) reveal differential vascular and interleukin signaling. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 109(8), p. 2802–2807. doi: 10.1073/pnas.1108781108.
• Sørlie, Therese (2011). How to personalise treatment in early breast cancer. European Journal of Cancer. ISSN 0959-8049. 47, p. S310–S311. doi: 10.1016/S0959-8049(11)70182-4.
• Borgan, Eldrid; Navon, Roy; Vollan, Hans Kristian Moen; Schlichting, Ellen; Sauer, Torill & Yakhini, Zohar [Show all 9 contributors for this article] (2011). Ischemia caused by time to freezing induces systematic microRNA and mRNA responses in cancer tissue. Molecular Oncology. ISSN 1574-7891. 5(6), p. 564–576. doi: 10.1016/j.molonc.2011.08.004.
• Akslen, Lars A.; Straume, Oddbjørn; Geisler, Stephanie; Sørlie, Therese; Chi, Jen-Tan Ashley & Aas, Turid [Show all 8 contributors for this article] (2011). Glomeruloid microvascular proliferation is associated with lack of response to chemotherapy in breast cancer. British Journal of Cancer. ISSN 0007-0920. 105(1), p. 9–12. doi: 10.1038/bjc.2011.203.
• Zhao, Xi; Rødland, Einar Andreas; Sørlie, Therese; Naume, Bjørn; Langerød, Anita & Frigessi, Arnoldo [Show all 9 contributors for this article] (2011). Combining Gene Signatures Improves Prediction of Breast Cancer Survival. PLOS ONE. ISSN 1932-6203. 6(3). doi: 10.1371/journal.pone.0017845.
• Russnes, Hege Elisabeth Giercksky; Vollan, Hans Kristian Moen; Lingjærde, Ole Christian; Krasnitz, Alexander; Lundin, Pär & Naume, Bjørn [Show all 23 contributors for this article] (2010). Genomic Architecture Characterizes Tumor Progression Paths and Fate in Breast Cancer Patients. Science Translational Medicine. ISSN 1946-6234. 2(38), p. 38–47. doi: 10.1126/scitranslmed.3000611.
• Muggerud, Aslaug; Hallett, Michael; Johnsen, Hilde; Kleivi, Kristine; Zhou, Wenjing & Tahmasebpoor, Simin [Show all 11 contributors for this article] (2010). Molecular diversity in ductal carcinoma in situ (DCIS) and early invasive breast cancer. Molecular Oncology. ISSN 1574-7891. 4(4), p. 357–368. doi: 10.1016/j.molonc.2010.06.007.
• Borgan, Eldrid; Sitter, Beathe; Lingjærde, Ole Christian; Johnsen, Hilde; Lundgren, Steinar & Bathen, Tone Frost [Show all 9 contributors for this article] (2010). Merging transcriptomics and metabolomics - advances in breast cancer profiling. BMC Cancer. ISSN 1471-2407. 10. doi: 10.1186/1471-2407-10-628.
• Myhre, Simen; Mohammed, Hayat; Tramm, Trine; Alsner, Jan; Finak, Greg & Park, Morag [Show all 10 contributors for this article] (2010). In Silico Ascription of Gene Expression Differences to Tumor and Stromal Cells in a Model to Study Impact on Breast Cancer Outcome. PLOS ONE. ISSN 1932-6203. 5(11). doi: 10.1371/journal.pone.0014002.
• Muggerud, Aslaug; Rønneberg, Jo Anders; Wärnberg, Fredrik; Botling, Johan; Busato, Florence & Jovanovic, Jovana [Show all 12 contributors for this article] (2010). Frequent aberrant DNA methylation of ABCB1, FOXC1, PPP2R2B and PTEN in ductal carcinoma in situ and early invasive breast cancer. Breast Cancer Research. ISSN 1465-5411. 12(1). doi: 10.1186/bcr2466.
• Potapenko, Ivan Olegovich; Haakensen, Vilde Drageset; Luders, Torben; Helland, Åslaug; Bukholm, Ida Rashida Khan & Sørlie, Therese [Show all 9 contributors for this article] (2010). Glycan gene expression signatures in normal and malignant breast tissue; possible role in diagnosis and progression. Molecular Oncology. ISSN 1574-7891. 4(2), p. 98–118. doi: 10.1016/j.molonc.2009.12.001.
• Podo, F; Buydens, LMC; Degani, H; Hilhorst, Riet; Klipp, Edda & Gribbestad, Ingrid S [Show all 18 contributors for this article] (2010). Triple-negative breast cancer: Present challenges and new perspectives. Molecular Oncology. ISSN 1574-7891. 4(3), p. 209–229. doi: 10.1016/j.molonc.2010.04.006.
• Agarwal, R; Gonzalez-Angulo, AM; Myhre, Simen; Carey, M; Lee, JS & Overgaard, Jens [Show all 19 contributors for this article] (2009). Integrative Analysis of Cyclin Protein Levels Identifies Cyclin B1 as a Classifier and Predictor of Outcomes in Breast Cancer. Clinical Cancer Research. ISSN 1078-0432. 15(11), p. 3654–3662. doi: 10.1158/1078-0432.CCR-08-3293.
• Bergamaschi, Anna; Hjortland, Geir Olav; Triulzi, T; Sørlie, Therese; Johnsen, Hilde & Ree, Anne Hansen [Show all 12 contributors for this article] (2009). Molecular profiling and characterization of luminal-like and basal-like in vivo breast cancer xenograft models. Molecular Oncology. ISSN 1574-7891. 3(5-6), p. 469–482. doi: 10.1016/j.molonc.2009.07.003.
• Muggerud, Aslaug Aamodt; Edgren, H; Wolf, M; Kleivi, Kristine; Dejeux, E & Tost, J [Show all 8 contributors for this article] (2009). Data integration from two microarray platforms identifies bi-allelic genetic inactivation of RIC8A in a breast cancer cell line. BMC Medical Genomics. ISSN 1755-8794. 2. doi: 10.1186/1755-8794-2-26.
• Zhou, WJ; Muggerud, Aslaug Aamodt; Vu, Phuong Ngoc Thi; Due, Eldri Undlien; Sørlie, Therese & Børresen-Dale, Anne-Lise [Show all 8 contributors for this article] (2009). Full sequencing of TP53 identifies identical mutations within in situ and invasive components in breast cancer suggesting clonal evolution. Molecular Oncology. ISSN 1574-7891. 3(3), p. 214–219. doi: 10.1016/j.molonc.2009.03.001.
• Bergamaschi, Anna; Tagliabue, Elda; Sørlie, Therese; Naume, Bjørn; Triulzi, Tiziana & Orlandi, Rosario [Show all 13 contributors for this article] (2008). Extracellular matrix signature identifies breast cancer subgroups with different clinical outcome. Journal of Pathology. ISSN 0022-3417. 214(3), p. 357–367. doi: 10.1002/path.2278.
• Nordgard, Silje H.; Nordgard, Silje H.; Alnæs, Grethe I.G.; Alnaes, GIG; Hihn, Björn & Lingjærde, Ole Chr. [Show all 13 contributors for this article] (2008). Pathway based analysis of SNPs with relevance to 5-FU therapy: Relation to intratumoral mRNA expression and survival. International Journal of Cancer. ISSN 0020-7136. 123(3), p. 577–585. doi: 10.1002/ijc.23541.
• Naume, Bjørn; Zhao, Xi; Synnestvedt, Marit; Borgen, Elin; Giercksky, Hege E & Lingjærde, Ole Christian [Show all 13 contributors for this article] (2007). Presence of bone marrow micrometastasis is associated with different recurrence risk within molecular subtypes of breast cancer. Molecular Oncology. ISSN 1574-7891. 1(2), p. 160–171. doi: 10.1016/j.molonc.2007.03.004. Show summary

  Expression profiles of primary breast tumors were investigated in relation to disseminated tumor cells (DTCs) in bone marrow (BM) in order to increase our understanding of the dissemination process. Tumors were classified into five pre-defined molecular subtypes, and presence of DTC identified (at median 85 months follow-up) a subgroup of luminal A patients with particular poor outcome (p = 0.008). This was not apparent for other tumor subtypes. Gene expression profiles associated with DTC and with systemic relapse for luminal A patients were identified. This study suggests that DTC in BM differentially distinguishes clinical outcome in patients with luminal A type tumors and that DTC-associated gene expression analysis may identify genes of potential importance in tumor dissemination.

• Kristensen, Vessela N.; Edvardsen, Hege; Tsalenko, Anya; Nordgard, Silje H.; Sørlie, Therese & Sharan, Roded [Show all 14 contributors for this article] (2006). Genetic variation in putative regulatory loci controlling gene expression in breast cancer. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 103(20), p. 7735–7740. Show summary

  Candidate single-nucleotide polymorphisms (SNPs) were analyzed for associations to an unselected whole genome pool of tumor mRNA transcripts in 50 unrelated patients with breast cancer. SNPs were selected from 203 candidate genes of the reactive oxygen species pathway. We describe a general statistical framework for the simultaneous analysis of gene expression data and SNP genotype data measured for the same cohort, which revealed significant associations between subsets of SNPs and transcripts, shedding light on the underlying biology. We identified SNPs in EGF, IL1A, MAPK8, XPC, SOD2, and ALOX12 that are associated with the expression patterns of a significant number of transcripts, indicating the presence of regulatory SNPs in these genes. SNPs were found to act in trans in a total of 115 genes. SNPs in 43 of these 115 genes were found to act both in cis and in trans. Finally, subsets of SNPs that share significantly many common associations with a set of transcripts (biclusters) were identified. The subsets of transcripts that are significantly associated with the same set of SNPs or to a single SNP were shown to be functionally coherent in Gene Ontology and pathway analyses and coexpressed in other independent data sets, suggesting that many of the observed associations are within the same functional pathways. To our knowledge, this article is the first study to correlate SNP genotype data in the germ line with somatic gene expression data in breast tumors. It provides the statistical framework for further genotype expression correlation studies in cancer data sets.

• Muggerud, Aslaug Aamodt; Johnsen, Hilde; Barnes, Debra A.; Steel, Adam; Lønning, Per Eystein & Lonning, PE [Show all 9 contributors for this article] (2006). Evaluation of MetriGenix custom 4D (TM) arrays applied for detection of breast cancer subtypes. BMC Cancer. ISSN 1471-2407. 6. doi: 10.1186/1471-2407-6-59.
• Sørlie, Therese; Perou, Charles M.; Fan, Cheng; Geisler, Stephanie; Aas, Turid & Nobel, Andrew [Show all 11 contributors for this article] (2006). Gene expression profiles do not consistently predict the clinical treatment response in locally advanced breast cancer. Molecular Cancer Therapeutics. ISSN 1535-7163. 5(11), p. 2914–2918. doi: 10.1158/1535-7163.MCT-06-0126.
• Kristensen, Vessela N.; Sørlie, Therese; Geisler, Jurgen; Langerød, Anita; Yoshimura, Noriko & Kåresen, Rolf [Show all 9 contributors for this article] (2005). Gene expression profiling of breast cancer in relation to estrogen receptor status and estrogen-metabolizing enzymes: Clinical implication. Clinical Cancer Research. ISSN 1078-0432. 11, p. 878s–883s.
• Sørlie, Therese; Johnsen, Hilde; Vu, Phuong Ngoc Thi; Lind, Gruo Elisabeth; Lothe, Ragnhild A & Børresen-Dale, Anne-Lise (2005). Mutation screening of the TP53 gene by temporal temperature gel electrophoresis (TTGE). Methods in molecular biology. ISSN 1064-3745. 291, p. 207–216.
• Kristensen, Vessela Nedelcheva ; Sorlie, Therese; Sørlie, Therese; Geisler, Jürgen; Geisler, E & Yoshimura, Noriko [Show all 14 contributors for this article] (2005). Effects of anastrozole on the intratumoral gene expression in locally advanced breast cancer. Journal of Steroid Biochemistry and Molecular Biology. ISSN 0960-0760. 95(1-5), p. 105–111. doi: 10.1016/j.jsbmb.2005.04.028.
• Sørlie, Therese; Børresen-Dale, Anne-Lise; Lønning, Per Eystein; Brown, Patrick O & Botstein, David (2004). Expression profiling of breast cancer: From molecular portraits to clinical utility. In Brenner, Charles & Duggan, David J (Ed.), Oncogenomics: Molecular approaches to cancer. John Wiley & Sons. ISSN 0-471-22592-4. p. 77–100.
• Schaner, M.E.; Ross, D.T.; Ciaravino, G.; Sørlie, Therese; Troyanskaya, O. & Diehn, M. [Show all 25 contributors for this article] (2003). Gene expression patterns in ovarian carcinomas. Molecular Biology of the Cell. ISSN 1059-1524. 14, p. 4376–4386.
• Sørlie, Therese; Tibshirani, Robert; Parker, Joel; Hastie, Trevor; Marron, J.S. & Nobel, Andrew [Show all 16 contributors for this article] (2003). Repeated observation of breast tumor subtypes in independent gene expression data sets. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 100(14), p. 8418–8423.
• Pollack, Jonathan R.; Sørlie, Therese; Perou, Charles M.; Rees, Christian A.; Jeffrey, Stefanie S. & Lønning, Per Eystein [Show all 10 contributors for this article] (2002). Microarray analysis reveals a major direct role of DNA copy number alteration in the transcriptional program of human breast tumors. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 99(20), p. 12963–12968. Show summary

  Genomic DNA copy number alterations are key genetic events in the development and progression of human cancers. Here we report a genome-wide microarray comparative genomic hybridization (array CGH) analysis of DNA copy number variation in a series of primary human breast tumors. We have profiled DNA copy number alteration across 6,691 mapped human genes, in 44 predominantly advanced, primary breast tumors and 10 breast cancer cell lines. While the overall patterns of DNA amplification and deletion corroborate previous cytogenetic studies, the high-resolution (gene-by-gene) mapping of amplicon boundaries and the quantitative analysis of amplicon shape provide significant improvement in the localization of candidate oncogenes. Parallel microarray measurements of mRNA levels reveal the remarkable degree to which variation in gene copy number contributes to variation in gene expression in tumor cells. Specifically, we find that 62% of highly amplified genes show moderately or highly elevated expression, that DNA copy number influences gene expression across a wide range of DNA copy number alterations (deletion, low-, mid- and high-level amplification), that on average, a 2-fold change in DNA copy number is associated with a corresponding 1.5-fold change in mRNA levels, and that overall, at least 12% of all the variation in gene expression among the breast tumors is directly attributable to underlying variation in gene copy number. These findings provide evidence that widespread DNA copy number alteration can lead directly to global deregulation of gene expression, which may contribute to the development or progression of cancer.

• Sørlie, Therese; Perou, Charles M.; Tibshirani, Robert; Aas, Turid; Geisler, Stephanie & Johnsen, Hilde [Show all 17 contributors for this article] (2001). Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 98(19), p. 10869–10874. Show summary

  The purpose of this study was to classify breast carcinomas based on variations in gene expression patterns derived from cDNA microarrays and to correlate tumor characteristics to clinical outcome. A total of 85 cDNA microarray experiments representing 78 cancers, three fibroadenomas, and four normal breast tissues were analyzed by hierarchical clustering. As reported previously, the cancers could be classified into a basal epithelial-like group, an ERBB2-overexpressing group and a normal breast-like group based on variations in gene expression. A novel finding was that the previously characterized luminal epithelial/estrogen receptor-positive group could be divided into at least two subgroups, each with a distinctive expression profile. These subtypes proved to be reasonably robust by clustering using two different gene sets: first, a set of 456 cDNA clones previously selected to reflect intrinsic properties of the tumors and, second, a gene set that highly correlated with patient outcome. Survival analyses on a subcohort of patients with locally advanced breast cancer uniformly treated in a prospective study showed significantly different outcomes for the patients belonging to the various groups, including a poor prognosis for the basal-like subtype and a significant difference in outcome for the two estrogen receptor-positive groups.

• Lønning, Per Eystein; Sørlie, Therese; Perou, Chuck M.; Brown, Patrick O.; Bostein, David & Børresen-Dale, Anne-Lise (2001). Microarrays in primary breast cancer -lessons from chemotherapy studies. Endocrine-Related Cancer. ISSN 1351-0088. 8, p. 259–263.

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• Lønning, Per Eystein; Sørlie, Therese & Børresen-Dale, Anne-Lise (2005). Genomics in breast cancer - therapeutic implications. Nature Publishing Group. 8 p.
• Sørlie, Therese; Vu, Phuong Ngoc Thi; Johnsen, Hilde; Lind, Gruo Elisabeth; Lothe, Ragnhild A & Børresen-Dale, Anne-Lise (2004). Mutation screening of the TP53 gene by Temporal Temperature Gel Electophoresis (TTGE). P. Keohavong and S. G. Grant. Human Press Inc.. ISBN 1064-3745. 291(*). 9 p.
• Sørlie, Therese; Børresen-Dale, Anne-Lise ; Lønning, Per Eystein; Brown, P. O & Botstein, David (2004). Expression profiling of breast cancer: From molecular portraits to clinical utility. John Wiley & Sons. 23 p.

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• Skrbo, Nirma; Kirik, U.; Kristian, Alexandr; Cifani, P.; Antberg, L. & Moestue, Siver Andreas [Show all 11 contributors for this article] (2016). Protein expression analysis of intratumor heterogeneity in a luminal-like breast cancer xenograft.
• Moestue, Siver Andreas; Grinde, Maria Tunset; Marangoni, Eilsabetta; Sørlie, Therese; Engebråten, Olav & Mælandsmo, Gunhild [Show all 8 contributors for this article] (2013). Cytosolic phospholipase A2 (cPLA2) as a therapeutic target in basal-like breast cancer.
• Moestue, Siver Andreas; Grinde, Maria Tunset; Marangoni, Elisabetta; Skrbo, Nirma; Rødland, Einar Andreas & Borgan, Eldrid [Show all 15 contributors for this article] (2013). Interplay of choline metabolites and genes in patient-derived breast cancer xenografts.
• Potapenko, Ivan Olegovich; Haakensen, Vilde Drageset; Luders, Torben; Helland, Åslaug; Bukholm, Ida Rashida Khan & Sørlie, Therese [Show all 9 contributors for this article] (2010). Glycan gene expression signatures distinguish normal and malignant breast tissue; possible role in diagnosis and progression. European Journal of Cancer. ISSN 0959-8049. 8(5), p. 102–102.
• Sørlie, Therese; Borgan, Eldrid; Myhre, Simen; Vollan, Hans Kristian Moen; Russnes, Hege Elisabeth Giercksky & Zhao, Xi [Show all 9 contributors for this article] (2010). The importance of gene-centring microarray data. The Lancet Oncology. ISSN 1470-2045. 11(8), p. 719–720.
• Børresen-Dale, Anne-Lise ; Sørlie, Therese & Kristensen, Vessela N. (2010). On the molecular biology of breast cancer Preface. Molecular Oncology. ISSN 1574-7891. 4(3), p. 171–173. doi: 10.1016/j.molonc.2010.04.007.
• Borgan, Eldrid; Sitter, Beathe; Lingjærde, Ole Christian; Johnsen, Hilde; Lundgren, Steinar & Bathen, Tone Frost [Show all 9 contributors for this article] (2010). Transcriptomics meets metabolomics - correlating snapshots of breast cancer metabolism. European Journal of Cancer. ISSN 0959-8049. 8(5), p. 140–140.
• Sørlie, Therese; Muggerud, Aslaug; Hallett, Michael; Johnsen, Hilde; Sahlberg, Kristine Kleivi & Botling, Johan [Show all 8 contributors for this article] (2010). Molecular diversity in ductal carcinoma in situ (DCIS) and early invasive breast cancer. European Journal of Cancer. ISSN 0959-8049. 8(5), p. 200–201.
• Potapenko, Ivan Olegovich; Haakensen, Vilde Drageset; Myhre, Simen; Luder, T; Naume, Bjørn & Lingjærde, Ole Christian [Show all 10 contributors for this article] (2009). Glycan signatures of breast cancer; possible role in diagnosis and progression of the disease. Annals of Oncology. ISSN 0923-7534. 20, p. 49–49.
• Sørlie, Therese (2009). Introducing Molecular Subtyping of Breast Cancer Into the Clinic? Journal of Clinical Oncology. ISSN 0732-183X. 27(8), p. 1153–1154. doi: 10.1200/JCO.2008.20.6276.
• Muggerud, Aslaug Aamodt; Sørlie, Therese; Kleivi, Kristine & Børresen-Dale, Anne-Lise (2009). Genetic and epigenetic alterations in breast tumour progression: The transition from in situ to invasive cancer. Unipub forlag. ISSN 978-82-8072-336-9.

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