Laura Cecilie Valestrand

• Valestrand, Laura Cecilie; Zheng, Fei; Hansen, Simen Hyll; Øgaard, Jonas; Hov, Johannes Espolin Roksund & Björkström, Niklas K. [Show all 9 contributors for this article] (2022). Bile from Patients with Primary Sclerosing Cholangitis Contains Mucosal-Associated Invariant T-Cell Antigens. American Journal of Pathology. ISSN 0002-9440. 192(4), p. 629–641. doi: 10.1016/j.ajpath.2021.12.008. Full text in Research Archive Show summary

  Primary sclerosing cholangitis (PSC) is associated with altered microbiota of the gut and bile. Mucosal-associated invariant T (MAIT) cells, enriched in human liver, uniquely recognize microbial-derived metabolites. This study aimed to determine whether bile from patients with PSC contains antigens activating MAIT cells. Bile was collected at the time of liver transplantation from patients with PSC (n = 28). The bile samples were either directly incubated with peripheral blood mononuclear cells from healthy donors or with antigen-presenting cells followed by co-culture with peripheral blood mononuclear cells. MAIT cell activation was assessed by flow cytometry. An anti-MR1 antibody was used to determine whether the activation was major histocompatibility complex class I–related protein (MR1) restricted. Biliary microbiota profiles were generated using 16S rRNA amplicon sequencing, and the abundance of the bacterial gene ribD was predicted. Eight of 28 bile samples could activate MAIT cells. This activation was partly MR1-dependent in five of eight bile samples. Microbial DNA was detected in 15 of 28 bile samples, including the five bile samples leading to MR1-dependent activation. A higher abundance of the ribD gene expression in the group of bile samples that could activate MAIT cells was predicted on the basis of the 16S sequencing. In co-culture experiments, cholangiocytes could take up and present biliary antigens to MAIT cells. These findings suggest a pathophysiological pathway in PSC connecting the immune system and the microbiome.

• Valestrand, Laura Cecilie; Berntsen, Natalie Lie; Fei, Zheng; Schrumpf, Erik; Hansen, Simen Hyll & Karlsen, Tom Hemming [Show all 10 contributors for this article] (2020). Lipid antigens in bile from patients with chronic liver diseases activate natural killer T cells. Clinical and Experimental Immunology. ISSN 0009-9104. doi: 10.1111/cei.13541. Full text in Research Archive
• Berntsen, Natalie Lie; Fosby, Bjarte; Tan, Corey; Reims, Henrik Mikael; Øgaard, Jonas & Jiang, Xiaojun [Show all 12 contributors for this article] (2018). Natural killer T cells mediate inflammation in the bile ducts. Mucosal Immunology. ISSN 1933-0219. p. 1–9. doi: 10.1038/s41385-018-0066-8.
• Sampaziotis, Fotios; Justin, Alexander W.; Tysoe, Olivia C.; Sawiak, Stephen; Godfrey, Edmund M. & Upponi, Sara S. [Show all 43 contributors for this article] (2017). Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids. Nature Medicine. ISSN 1078-8956. 23(8), p. 954–963. doi: 10.1038/nm.4360.
• Berntsen, Natalie Lie; Fosby, Bjarte; Valestrand, Laura Cecilie; Tan, Corey; Reims, Henrik Mikael & Schrumpf, Elisabeth [Show all 9 contributors for this article] (2017). Establishment of a surgical bile duct injection technique giving direct access to the bile ducts for studies of the murine biliary tree. American Journal of Physiology - Gastrointestinal and Liver Physiology. ISSN 0193-1857. 314(3), p. G349–G359. doi: 10.1152/ajpgi.00124.2017.
• Schrumpf, Elisabeth; Kummen, Martin; Valestrand, Cecilie; Greiner, Thomas U; Holm, Kristian & Arulampalam, Velmurugesam [Show all 13 contributors for this article] (2017). The gut microbiota contributes to a mouse model of spontaneous bile duct inflammation. Journal of Hepatology. ISSN 0168-8278. 66(2), p. 382–389. doi: 10.1016/j.jhep.2016.09.020. Full text in Research Archive

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