We do this by using human material, different murine models of cholangitis, sequening based approaches, organoids, organ-on-a-chip systems and other in vitro assays.
About the group
Our group is focusing on experimental and translational studies related to primary sclerosing cholangitis (PSC).
The overall aim of our research is to understand mechanisms regulating cholangitis with a clear focus on immunology and the interaction of the immune system with the microbiome and what role the cholangiocytes play in propagation of the inflammatory process.
From the previous genome-wide association studies it is clear that the genetic risk factors for PSC are genes involved in the immune response.
This inspired our clear focus on immunological mechanisms. We have therefore chosen to study various immune driven models of cholangitis along with models based on specific genetic findings in the human studies.
To uncover novel mechanisms, we are using unbiased spatial transcriptomics and single-cell sequencing.
To complement the in vivo studies the group is also using selected in vitro cellular models to study the role of various cell types in the liver and bile duct immune responses.
We are also aiming to model the disease process in the bile ducts using organoids and organ-on-a-chip technology. Such a bile-duct-on-a-chip model gives the opportunity to use organoids generated from patients in the experiments.
Our laboratory activities take place at the Research Institute of Internal Medicine.
Financial support
The experimental projects are funded by:
- The Norwegian PSC research center (NoPSC)
- PSC partners
- The South Eastern Norwegian Health trust
- The Research Council of Norway (NFR)
Publications
These are the group's latest publications in PubMed.
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T cells with increased responsiveness cause obesity in mice without diet intervention Mar. 29, 2024 10:00 AMGregersen I, Kong XY, Kooijman S, Foyn H, Grannes H, Olsen MB, Lone AM, Yang K, Quiles-Jiménez A, Tran M, Øgaard J, Segers FM, Rashidi A, Sagen EL, Lauritzen KH, Pronk ACM, de Boer JF, Holven KB, Melum E, Aukrust P, Taskén K, Holm S, Rensen PCN, Dahl TB, Halvorsen B (2024) T cells with increased responsiveness cause obesity in mice without diet intervention iScience, 27 (4), 109471 PubMed 38551005 DOI 10.1016/j.isci.2024.109471
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Cell-Free Microbial DNA Analysis: Effects of Blood Plasma and Serum Quantity, Biobanking Protocols, and Isolation Kits Feb. 28, 2024 10:00 AMNikitina D, Lukosevicius R, Tilinde D, Muskieta T, Hov JR, Melum E, Klovins J, Org E, Kiudelis G, Kupcinskas J, Skieceviciene J (2024) Cell-Free Microbial DNA Analysis: Effects of Blood Plasma and Serum Quantity, Biobanking Protocols, and Isolation Kits Biopreserv Biobank PubMed 38416864 DOI 10.1089/bio.2023.0048
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Author Correction: CEACAM1 regulates TIM-3-mediated tolerance and exhaustion Feb. 9, 2024 10:00 AMHuang YH, Zhu C, Kondo Y, Anderson AC, Gandhi A, Russell A, Dougan SK, Petersen BS, Melum E, Pertel T, Clayton KL, Raab M, Chen Q, Beauchemin N, Yazaki PJ, Pyzik M, Ostrowski MA, Glickman JN, Rudd CE, Ploegh HL, Franke A, Petsko GA, Kuchroo VK, Blumberg RS (2024) Author Correction: CEACAM1 regulates TIM-3-mediated tolerance and exhaustion Nature, 626 (8001), E19 PubMed 38336833 DOI 10.1038/s41586-024-07164-y
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Microbial exposure during early life regulates development of bile duct inflammation Nov. 24, 2023 10:00 AMOldereid TS, Jiang X, Øgaard J, Schrumpf E, Bjørnholt JV, Rasmussen H, Melum E (2024) Microbial exposure during early life regulates development of bile duct inflammation Scand J Gastroenterol, 59 (2), 192-201 PubMed 37997753 DOI 10.1080/00365521.2023.2278423
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Cholangiocytes Modulate CD100 Expression in the Liver and Facilitate Pathogenic T-Helper 17 Cell Differentiation Nov. 23, 2023 10:00 AMJiang X, Otterdal K, Chung BK, Maucourant C, Rønneberg JD, Zimmer CL, Øgaard J, Boichuk Y, Holm S, Geanon D, Schneditz G, Bergquist A, Björkström NK, Melum E (2023) Cholangiocytes Modulate CD100 Expression in the Liver and Facilitate Pathogenic T-Helper 17 Cell Differentiation Gastroenterology, 166 (4), 667-679 PubMed 37995866 DOI 10.1053/j.gastro.2023.11.283
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Role of bacteria and microbial metabolites in immune modulation during early life Mar. 5, 2024 10:00 AMOldereid TS, Jiang X, Nordhus KS, Ponzetta A, Bjørnholt JV, Björkström NK, Melum E, Rasmussen H (2023) Role of bacteria and microbial metabolites in immune modulation during early life Scand J Immunol, 99 (2), e13336 PubMed 38441196 DOI 10.1111/sji.13336
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Scalable production of tissue-like vascularized liver organoids from human PSCs Aug. 31, 2023 11:00 AMHarrison SP, Siller R, Tanaka Y, Chollet ME, de la Morena-Barrio ME, Xiang Y, Patterson B, Andersen E, Bravo-Pérez C, Kempf H, Åsrud KS, Lunov O, Dejneka A, Mowinckel MC, Stavik B, Sandset PM, Melum E, Baumgarten S, Bonanini F, Kurek D, Mathapati S, Almaas R, Sharma K, Wilson SR, Skottvoll FS, Boger IC, Bogen IL, Nyman TA, Wu JJ, Bezrouk A, Cizkova D, Corral J, Mokry J, Zweigerdt R, Park IH, Sullivan GJ (2023) Scalable production of tissue-like vascularized liver organoids from human PSCs Exp Mol Med, 55 (9), 2005-2024 PubMed 37653039 DOI 10.1038/s12276-023-01074-1
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Clinical and biochemical impact of vitamin B6 deficiency in primary sclerosing cholangitis before and after liver transplantation June 16, 2023 11:00 AMBraadland PR, Bergquist A, Kummen M, Bossen L, Engesæter LK, Reims HM, Björk I, Grzyb K, Abildgaard A, Småstuen MC, Folseraas T, Trøseid M, Ulvik A, Ueland PM, Melum E, Line PD, Høivik ML, Grønbæk H, Karlsen TH, Vesterhus M, Hov JR (2023) Clinical and biochemical impact of vitamin B6 deficiency in primary sclerosing cholangitis before and after liver transplantation J Hepatol, 79 (4), 955-966 PubMed 37328069 DOI 10.1016/j.jhep.2023.05.038
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Long-term survival after liver transplantation in patients with common variable immunodeficiency Mar. 18, 2023 10:00 AMAndersen IM, Reims HM, Grzyb K, Fevang B, Melum E, Aukrust P, Wiencke K, Nordøy I, Line PD, Jørgensen SF (2023) Long-term survival after liver transplantation in patients with common variable immunodeficiency Liver Transpl, 29 (8), 894-899 PubMed 36932715 DOI 10.1097/LVT.0000000000000130
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Pump-less, recirculating organ-on-a-chip (rOoC) platform Jan. 19, 2023 10:00 AMBusek M, Aizenshtadt A, Koch T, Frank A, Delon L, Martinez MA, Golovin A, Dumas C, Stokowiec J, Gruenzner S, Melum E, Krauss S (2023) Pump-less, recirculating organ-on-a-chip (rOoC) platform Lab Chip, 23 (4), 591-608 PubMed 36655405 DOI 10.1039/d2lc00919f