About the lecture
Single cell Ig gene amplification and sequencing is increasingly used for the molecular assessment of adaptive antigen receptor repertoires. We have developed a platform for the high-throughput analysis of Ig gene repertoires that preserves natural IgH and IgL gene associations and provides full-length Ig gene sequence information for direct gene cloning and the production of recombinant monoclonal antibodies. I will discuss how we use this platform to study the clonal evolution and functional quality of human B cell immune responses after repeated controlled exposure to a complex pathogen, the malaria parasite Plasmodium falciparum.