Sindre Lee-Ødegård

• Borgen, Tove Tveitan; Lee-Ødegård, Sindre; Fink Eriksen, Barbara & Eriksen, Erik Fink (2024). Intermittent Dosing of Zoledronic acid based on bone turnover marker assessment reduces vertebral and non-vertebral fractures . JBMR Plus. ISSN 2473-4039. doi: 10.1093/jbmrpl/ziae072.
• Kvist , Ahalya Anita Suntharalingam ; Sharma, Archana; Sommer, Christine; Qvigstad, Elisabeth; Gulseth, Hanne Løvdal & Sollid, Stina Therese [Vis alle 12 forfattere av denne artikkelen] (2024). Adipose Tissue Insulin Resistance in South Asian and Nordic Women after Gestational Diabetes Mellitus. Metabolites. ISSN 2218-1989. 14(5). doi: 10.3390/metabo14050288.
• Olsen, Thomas; Vinknes, Kathrine Jørgensen; Barvíková, Kristýna; Stolt, Emma Theresia Kjelland; Lee-Ødegård, Sindre & Troensegaard, Hannibal [Vis alle 18 forfattere av denne artikkelen] (2024). Dietary sulfur amino acid restriction in humans with overweight and obesity: Evidence of an altered plasma and urine sulfurome, and a novel metabolic signature that correlates with loss of fat mass and adipose tissue gene expression. Redox Biology. ISSN 2213-2317. 73. doi: 10.1016/j.redox.2024.103192.
• Linares-Pineda, Teresa M.; Fragoso Bargas, Nicolas; Picón, María José; Molina-Vega, Maria; Jenum, Anne Karen & Sletner, Line [Vis alle 14 forfattere av denne artikkelen] (2024). DNA methylation risk score for type 2 diabetes is associated with gestational diabetes. Cardiovascular Diabetology. ISSN 1475-2840. 23(1). doi: 10.1186/s12933-024-02151-z.
• Opsahl, J. O.; Fragoso-Bargas, N.; Lee, Y.; Carlsen, E. Ø.; Lekanova, N. & Qvigstad, E. [Vis alle 13 forfattere av denne artikkelen] (2024). Epigenome-wide association study of DNA methylation in maternal blood leukocytes with BMI in pregnancy and gestational weight gain. International Journal of Obesity. ISSN 0307-0565. s. 1–10. doi: 10.1038/s41366-024-01458-x. Fulltekst i vitenarkiv
• Olsen, Thomas; Stolt, Emma Theresia Kjelland; Øvrebø, Bente; Elshorbagy, Amany; Tore, Elena C. & Lee-Ødegård, Sindre [Vis alle 20 forfattere av denne artikkelen] (2024). Dietary sulfur amino acid restriction in humans with overweight and obesity: a translational randomized controlled trial. Journal of Translational Medicine. ISSN 1479-5876. 22(1). doi: 10.1186/s12967-023-04833-w.
• Jersin, Regine Åsen; Tallapragada, Divya Sri; Skartveit, Linn; Bjune, Mona Synnøve; Muniandy, Maheswary & Lee-Ødegård, Sindre [Vis alle 16 forfattere av denne artikkelen] (2023). Impaired Adipocyte SLC7A10 Promotes Lipid Storage in Association With Insulin Resistance and Altered BCAA Metabolism. Journal of Clinical Endocrinology and Metabolism (JCEM). ISSN 0021-972X. 108(9), s. 2217–2229. doi: 10.1210/clinem/dgad148. Fulltekst i vitenarkiv
• Moore, Timothy M.; Lee-Ødegård, Sindre; Olsen, Thomas; Morselli, Marco; Strumwasser, Alexander R. & Lin, Amanda J. [Vis alle 40 forfattere av denne artikkelen] (2023). Conserved multi-tissue transcriptomic adaptations to exercise training in humans and mice. Cell reports. ISSN 2211-1247. 42(5). doi: 10.1016/j.celrep.2023.112499. Fulltekst i vitenarkiv
• Fragoso-Bargas, Nicolas; Page, Christian Magnus; Joubert, Bonnie R.; London, Stephanie J.; Lee-Ødegård, Sindre & Opsahl, Julia Onsrud [Vis alle 13 forfattere av denne artikkelen] (2023). Epigenome-wide association study of serum folate in maternal peripheral blood leukocytes. Epigenomics. ISSN 1750-1911. 15(1), s. 39–52. doi: 10.2217/epi-2022-0427. Fulltekst i vitenarkiv
• Fragoso-Bargas, Nicolas; Elliott, Hannah R.; Lee-Ødegård, Sindre; Opsahl, Julia Onsrud; Sletner, Line & Jenum, Anne Karen [Vis alle 12 forfattere av denne artikkelen] (2022). Cross-Ancestry DNA Methylation Marks of Insulin Resistance in Pregnancy: An Integrative Epigenome-Wide Association Study. Diabetes. ISSN 0012-1797. 72(3), s. 415–426. doi: 10.2337/db22-0504.
• Sommer, Christine; Vangberg, Kjersti G; Moen, Gunn-Helen Øiseth; Evans, David M.; Lee-Ødegård, Sindre & Høgestøl, Ingvild Kristine [Vis alle 11 forfattere av denne artikkelen] (2022). Insulin and Body Mass Index Decrease Serum Soluble Leptin Receptor Levels in Humans. Journal of Clinical Endocrinology and Metabolism (JCEM). ISSN 0021-972X. 108(5), s. 1110–1119. doi: 10.1210/clinem/dgac699. Fulltekst i vitenarkiv
• Elshorbagy, Amany; Bastani, Nasser Ezzatkhah; Lee-Ødegård, Sindre; Øvrebø, Bente; Haj Yasein, Nadia & Svendsen, Karianne [Vis alle 10 forfattere av denne artikkelen] (2022). The association of fasting plasma thiol fractions with body fat compartments, biomarker profile, and adipose tissue gene expression. Amino Acids. ISSN 0939-4451. doi: 10.1007/s00726-022-03229-2. Fulltekst i vitenarkiv
• Sharma, Archana; Lee-Ødegård, Sindre; Qvigstad, Elisabeth; Sommer, Christine; Sattar, Naveed & Gill, Jason M.R. [Vis alle 10 forfattere av denne artikkelen] (2022). β-Cell Function, Hepatic Insulin Clearance, and Insulin Sensitivity in South Asian and Nordic Women After Gestational Diabetes Mellitus. Diabetes. ISSN 0012-1797. 71(12), s. 2530–2538. doi: 10.2337/db22-0622. Fulltekst i vitenarkiv
• Lee-Ødegård, Sindre; Ueland, Thor; Thorsby, Per Medbøe; Aukrust, Pål; Michelsen, Annika Elisabet & Halvorsen, Bente [Vis alle 8 forfattere av denne artikkelen] (2022). Fetuin-A mediates the difference in adipose tissue insulin resistance between young adult pakistani and norwegian patients with type 2 diabetes. BMC Endocrine Disorders. ISSN 1472-6823. 22. doi: 10.1186/s12902-022-01127-y. Fulltekst i vitenarkiv Vis sammendrag

  Background South-Asian immigrants to Western countries have a high prevalence of type 2 diabetes mellitus (T2DM) and increased adipose tissue insulin resistance (AT-IR), as compared to their Western counterparts. Fetuin-A is a hepatokine known to influence AT-IR. Aim Can plasma fetuin-A concentrations explain an ethnic difference in adipose tissue insulin resistance? Methods We performed a two-step euglycemic-hyperinsulinaemic clamp and measured plasma concentrations of fetuin-A and non-esterified fatty acids (NEFA), in 18 Pakistani and 21 Norwegians with T2DM (age 29–45y) in Norway. AT-IR was calculated as NEFA-suppression during the clamp. The adipokines/cytokines leptin, adiponectin, visfatin, PTX3, IL-1β, INF-γ, and IL-4 were measured in fasting plasma. Liver fat was estimated by CT-scans. Results Despite a lower BMI, Pakistani patients displayed higher AT-IR than Norwegians. NEFA-suppression during clamp was lower in Pakistani than Norwegians (mean=-20.6%, 95%CI=[-40.8, -0.01] and p = 0.046). Plasma fetuin-A concentration was higher in Pakistani than Norwegians (43.4 ng/mL[12.7,74.0], p = 0.007) and correlated negatively to %NEFA-suppression during clamp (rho=-0.39, p = 0.039). Plasma fetuin-A concentration explained 22% of the ethnic difference in NEFA-suppression during the clamp. Pakistani patients exhibited higher plasma leptin and lower PTX3 levels than Norwegian, and plasma visfatin correlated positively to plasma fetuin-A levels in the Pakistani patients. We observed no correlation between plasma fetuin-A and liver fat, but fetuin-A correlated negatively with plasma IL-1β, INF-γ, and IL-4 concentrations. Plasma IL-4 concentration was lower in Pakistani than in Norwegian patients. Conclusion Fetuin-A may contribute to explain the discrepancy in T2DM prevalence between Pakistani and Norwegians patients by influencing AT-IR.

• Bjerkreim, Betty Ann; Hammerstad, Sara Salehi; Gulseth, Hanne Løvdal; Berg, Tore Julsrud; Lee-Ødegård, Sindre & Eriksen, Erik Fink (2022). Thyroid Signaling Biomarkers in Female Symptomatic Hypothyroid Patients on Liothyronine versus Levothyroxine Monotherapy: A Randomized Crossover Trial. Journal of Thyroid Research. ISSN 2090-8067. 2022. doi: 10.1155/2022/6423023. Fulltekst i vitenarkiv
• Doncheva, Atanaska Ivanova; Romero, Silvana Angelica Troncoso; Ramirez-Garrastacho, Manuel; Lee-Ødegård, Sindre; Kolnes, Kristoffer Jensen & Tangen, Daniel S. [Vis alle 11 forfattere av denne artikkelen] (2022). Extracellular vesicles and microRNAs are altered in response to exercise, insulin sensitivity and overweight. Acta Physiologica. ISSN 1748-1708. 236(4). doi: 10.1111/apha.13862. Fulltekst i vitenarkiv
• Lee-Ødegård, Sindre; Olsen, Thomas; Norheim, Frode Amador; Drevon, Christian A & Birkeland, Kåre Inge (2022). Potential Mechanisms for How Long-Term Physical Activity May Reduce Insulin Resistance. Metabolites. ISSN 2218-1989. 12(3)(208). doi: 10.3390/metabo12030208. Fulltekst i vitenarkiv
• Fragoso-Bargas, Nicolas; Opsahl, Julia Onsrud; Kiryushchenko, Nadezhda; Böttcher, Yvonne; Lee-Ødegård, Sindre & Qvigstad, Elisabeth [Vis alle 15 forfattere av denne artikkelen] (2021). Cohort profile: Epigenetics in Pregnancy (EPIPREG) – Population-based sample of European and South Asian pregnant women with epigenome-wide DNA methylation (850k) in peripheral blood leukocytes. PLOS ONE. ISSN 1932-6203. 16(8), s. 1–15. doi: 10.1371/journal.pone.0256158. Fulltekst i vitenarkiv
• Bjerkreim, Betty Ann; Hammerstad, Sara Salehi; Gulseth, Hanne Løvdal; Berg, Tore Julsrud; Lee-Ødegård, Sindre & Rangberg, Anbjørg [Vis alle 12 forfattere av denne artikkelen] (2021). Effect of Liothyronine Treatment on Quality of Life in Female Hypothyroid Patients With Residual Symptoms on Levothyroxine Therapy: A Randomized Crossover Study. Frontiers in Endocrinology. ISSN 1664-2392. 13, s. 1–11. doi: 10.3389/fendo.2022.816566. Fulltekst i vitenarkiv
• Bleskestad, Kristiane Birkeland; Nordheim, Espen; Lindahl, Jørn Petter Hanto; Midtvedt, Karsten; Pihlstrøm, Hege K. & Horneland, Rune [Vis alle 10 forfattere av denne artikkelen] (2021). Insulin secretion and action after pancreas transplantation. A retrospective single-center study. Scandinavian Journal of Clinical and Laboratory Investigation. ISSN 0036-5513. 81(5), s. 365–370. doi: 10.1080/00365513.2021.1926535. Fulltekst i vitenarkiv
• Tallay, Elias; Lindberg, Sondre K.; Lee-Ødegård, Sindre; Bjordal, Jonas; Fraser, Alexander Nilsskog & Madsen, Jan Erik [Vis alle 7 forfattere av denne artikkelen] (2021). External validity in a multicenter randomized clinical trial of proximal humeral fractures: the DelPhi trial. European Journal of Orthopaedic Surgery & Traumatology (EJOST). ISSN 1633-8065. doi: 10.1007/s00590-021-02982-6.
• Lee, Yunsung; Jacobsen, Kristine Løkås; Denault, William Robert Paul; Nustad, Haakon Egdetveit; Page, Christian & Lyle, Robert [Vis alle 19 forfattere av denne artikkelen] (2020). Blood-based epigenetic estimators of chronological age in human adults using DNA methylation data from the Illumina MethylationEPIC array. BMC Genomics. ISSN 1471-2164. 21(1). doi: 10.1186/s12864-020-07168-8. Fulltekst i vitenarkiv
• Lee-Ødegård, Sindre; Gulseth, Hanne Løvdal; Langleite, Torgrim Mikal; Norheim, Frode; Olsen, Thomas & Refsum, Helga [Vis alle 9 forfattere av denne artikkelen] (2020). Branched-chain amino acid metabolism, insulin sensitivity and liver fat response to exercise training in sedentary dysglycaemic and normoglycaemic men. Diabetologia. ISSN 0012-186X. 64, s. 410–423. doi: 10.1007/s00125-020-05296-0. Fulltekst i vitenarkiv
• Zhou, Zhenqi; Moore, Timothy M.; Drew, Brian G.; Ribas, Vicent; Wanagat, Jonathan & Civelek, Mete [Vis alle 31 forfattere av denne artikkelen] (2020). Estrogen receptor α controls metabolism in white and brown adipocytes by regulating Polg1 and mitochondrial remodeling. Science Translational Medicine. ISSN 1946-6234. 12(555). doi: 10.1126/SCITRANSLMED.AAX8096. Fulltekst i vitenarkiv Vis sammendrag

  Obesity is heightened during aging, and although the estrogen receptor  (ER) has been implicated in the prevention of obesity, its molecular actions in adipocytes remain inadequately understood. Here, we show that adipose tissue ESR1/Esr1 expression inversely associated with adiposity and positively associated with genes involved in mitochondrial metabolism and markers of metabolic health in 700 Finnish men and 100 strains of inbred mice from the UCLA Hybrid Mouse Diversity Panel. To determine the anti-obesity actions of ER in fat, we selectively deleted Esr1 from white and brown adipocytes in mice. In white adipose tissue, Esr1 controlled oxidative metabolism by restraining the targeted elimination of mitochondria via the E3 ubiquitin ligase parkin. mtDNA content was elevated, and adipose tissue mass was reduced in adipose-selective parkin knockout mice. In brown fat centrally involved in body temperature maintenance, Esr1 was requisite for both mitochondrial remodeling by dynamin-related protein 1 (Drp1) and uncoupled respiration thermogenesis by uncoupled protein 1 (Ucp1). In both white and brown fat of female mice and adipocytes in culture, mitochondrial dysfunction in the context of Esr1 deletion was paralleled by a reduction in the expression of the mtDNA polymerase γ subunit Polg1. We identified Polg1 as an ER target gene by showing that ER binds the Polg1 promoter to control its expression in 3T3L1 adipocytes. These findings support strategies leveraging ER action on mitochondrial function in adipocytes to combat obesity and metabolic dysfunction.

• Hansen, Anne-Marte; Wium, Cecilie; Lee-Ødegård, Sindre; Tierney, Audrey C.; McCarthy, Danielle & Roche, Helen M. [Vis alle 9 forfattere av denne artikkelen] (2020). Substantial inter-individual variations in insulin secretion and sensitivity across the glucometabolic spectrum. Scandinavian Journal of Clinical and Laboratory Investigation. ISSN 0036-5513. 80(4), s. 282–290. doi: 10.1080/00365513.2020.1730433. Fulltekst i vitenarkiv
• Olsen, Thomas; Øvrebø, Bente; Haj Yasein, Nadia; Lee-Ødegård, Sindre; Svendsen, Karianne & Hjorth, Marit [Vis alle 11 forfattere av denne artikkelen] (2020). Effects of dietary methionine and cysteine restriction on plasma biomarkers, serum fibroblast growth factor 21, and adipose tissue gene expression in women with overweight or obesity: a double-blind randomized controlled pilot study. Journal of Translational Medicine. ISSN 1479-5876. 18(122). doi: 10.1186/s12967-020-02288-x. Fulltekst i vitenarkiv
• Lee-Ødegård, Sindre; Kolset, Svein Olav; Birkeland, Kåre I.; Drevon, Christian A & Reine, Trine M. (2019). Acute exercise increases syndecan-1 and -4 serum concentrations. Glycoconjugate Journal. ISSN 0282-0080. 36(2), s. 113–125. doi: 10.1007/s10719-019-09869-z. Fulltekst i vitenarkiv
• Blakstad, Elin Wahl; Korpela, Katri; Lee-Ødegård, Sindre; Nakstad, Britt; Moltu, Sissel & Strømmen, Kenneth [Vis alle 11 forfattere av denne artikkelen] (2019). Enhanced nutrient supply and intestinal microbiota development in very low birth weight infants. Pediatric Research. ISSN 0031-3998. doi: 10.1038/s41390-019-0412-x.
• Lee-Ødegård, Sindre; Norheim, Frode; Langleite, Torgrim Mikal; Gulseth, Hanne Løvdal; Birkeland, Kåre I. & Drevon, Christian A (2019). Effects of long-term exercise on plasma adipokine levels and inflammation-related gene expression in subcutaneous adipose tissue in sedentary dysglycaemic, overweight men and sedentary normoglycaemic men of healthy weight. Diabetologia. ISSN 0012-186X. 62(6), s. 1048–1064. doi: 10.1007/s00125-019-4866-5. Fulltekst i vitenarkiv
• Lee-Ødegård, Sindre; Olsen, Thomas; Vinknes, Kathrine; Refsum, Helga; Gulseth, Hanne Løvdal & Birkeland, Kåre I. [Vis alle 7 forfattere av denne artikkelen] (2019). Plasma Sulphur-Containing Amino Acids, Physical Exercise and Insulin Sensitivity in Overweight Dysglycemic and Normal Weight Normoglycemic Men. Nutrients. ISSN 2072-6643. 11(1). doi: 10.3390/nu11010010. Fulltekst i vitenarkiv
• Moore, Timothy M.; Zhou, Zhenqi; Cohn, Whitaker; Norheim, Frode; Lin, Amanda J. & Kalajian, Nareg [Vis alle 23 forfattere av denne artikkelen] (2018). The impact of exercise on mitochondrial dynamics and the role of Drp1 in exercise performance and training adaptations in skeletal muscle. Molecular Metabolism. ISSN 2212-8778. 21, s. 51–67. doi: 10.1016/j.molmet.2018.11.012. Fulltekst i vitenarkiv
• Lee-Ødegård, Sindre; Norheim, Frode; Gulseth, Hanne Løvdal; Langleite, Torgrim Mikal; Aker, Andreas Helge Mikael & Gundersen, Thomas Erik [Vis alle 9 forfattere av denne artikkelen] (2018). Skeletal muscle phosphatidylcholine and phosphatidylethanolamine respond to exercise and influence insulin sensitivity in men. Scientific Reports. ISSN 2045-2322. 8:6531, s. 1–12. doi: 10.1038/s41598-018-24976-x. Fulltekst i vitenarkiv
• Pourteymour, Shirin; Hjorth, Marit; Lee-Ødegård, Sindre; Holen, Torgeir; Langleite, Torgrim Mikal & Jensen, Jørgen [Vis alle 9 forfattere av denne artikkelen] (2017). Dual specificity phosphatase 5 and 6 are oppositely regulated in human skeletal muscle by acute exercise. Physiological Reports. ISSN 2051-817X. 5:e13459(19), s. 1–14. doi: 10.14814/phy2.13459. Fulltekst i vitenarkiv
• Lee, Sindre; Norheim, Frode; Gulseth, Hanne Løvdal; Langleite, Torgrim Mikal; Kolnes, Kristoffer Jensen & Tangen, Daniel S. [Vis alle 12 forfattere av denne artikkelen] (2017). Interaction between plasma fetuin-A and free fatty acids predicts changes in insulin sensitivity in response to long-term exercise. Physiological Reports. ISSN 2051-817X. 5(5). doi: 10.14814/phy2.13183. Fulltekst i vitenarkiv Vis sammendrag

  The hepatokine fetuin‐A can together with free fatty acids (FFAs) enhance adipose tissue (AT) inflammation and insulin resistance via toll‐like receptor 4 (TLR4). Although some of the health benefits of exercise can be explained by altered release of myokines from the skeletal muscle, it is not well documented if some of the beneficial effects of exercise can be explained by altered secretion of hepatokines. The aim of this study was to examine the effect of interaction between fetuin‐A and FFAs on insulin sensitivity after physical exercise. In this study, 26 sedentary men who underwent 12 weeks of combined endurance and strength exercise were included. Insulin sensitivity was measured using euglycemic‐hyperinsulinemic clamp, and AT insulin resistance was indicated by the product of fasting plasma concentration of FFAs and insulin. Blood samples and biopsies from skeletal muscle and subcutaneous AT were collected. Several phenotypic markers were measured, and mRNA sequencing was performed on the biopsies. AT macrophages were analyzed based on mRNA markers. The intervention improved hepatic parameters, reduced plasma fetuin‐A concentration (~11%, P < 0.01), slightly changed FFAs concentration, and improved glucose infusion rate (GIR) (~33%, P < 0.01) across all participants. The change in circulating fetuin‐A and FFAs interacted to predict some of the change in GIR (β = −42.16, P = 0.030), AT insulin resistance (β = 0.579, P = 0.003), gene expression related to TLR‐signaling in AT and AT macrophage mRNA (β = 94.10, P = 0.034) after exercise. We observed no interaction effects between FFAs concentrations and leptin and adiponectin on insulin sensitivity, or any interaction effects between Fetuin‐A and FFAs concentrations on skeletal muscle TLR‐signaling. The relationship between FFAs levels and insulin sensitivity seemed to be specific for fetuin‐A and the AT. Some of the beneficial effects of exercise on insulin sensitivity may be explained by changes in circulating fetuin‐A and FFAs, promoting less TLR4 signaling in AT perhaps by modulating AT macrophages.

• Pourteymour, Shirin; Eckardt, Kristin; Holen, Torgeir; Langleite, Torgrim Mikal; Lee, Sindre & Jensen, Jørgen [Vis alle 9 forfattere av denne artikkelen] (2017). Global mRNA sequencing of human skeletal muscle: Search for novel exercise-regulated myokines. Molecular Metabolism. ISSN 2212-8778. 6(4), s. 352–365. doi: 10.1016/j.molmet.2017.01.007. Fulltekst i vitenarkiv Vis sammendrag

  Objective: Skeletal muscle is an important secretory organ, producing and releasing numerous myokines, which may be involved in mediating beneficial health effects of physical activity. More than 100 myokines have been identified by different proteomics approaches, but these techniques may not detect all myokines. We used mRNA sequencing as an untargeted approach to study gene expression of secreted proteins in skeletal muscle upon acute as well as long-term exercise. Methods: Twenty-six middle-aged, sedentary men underwent combined endurance and strength training for 12 weeks. Skeletal muscle biopsies from m. vastus lateralis and blood samples were taken before and after an acute bicycle test, performed at baseline as well as after 12 weeks of training intervention. We identified transcripts encoding secretory proteins that were changed more than 1.5-fold in muscle after exercise. Secretory proteins were defined based on either curated UniProt annotations or predictions made by multiple bioinformatics methods. Results: This approach led to the identification of 161 candidate secretory transcripts that were up-regulated after acute exercise and 99 that where increased after 12 weeks exercise training. Furthermore, 92 secretory transcripts were decreased after acute and/or long-term physical activity. From these responsive transcripts, we selected 17 candidate myokines sensitive to short- and/or long-term exercise that have not been described as myokines before. The expression of these transcripts was confirmed in primary human skeletal muscle cells during in vitro differentiation and electrical pulse stimulation (EPS). One of the candidates we identified was macrophage colony-stimulating factor-1 (CSF1), which influences macrophage homeostasis. CSF1 mRNA increased in skeletal muscle after acute and long-term exercise, which was accompanied by a rise in circulating CSF1 protein. In cultured muscle cells, EPS promoted a significant increase in the expression and secretion of CSF1. Conclusion: We identified 17 new, exercise-responsive transcripts encoding secretory proteins. We further identified CSF1 as a novel myokine, which is secreted from cultured muscle cells and up-regulated in muscle and plasma after acute exercise.

• Lee, Sindre; Norheim, Frode; Langleite, Torgrim Mikal; NORENG, HANS JØRGEN; Storås, Tryggve & Afman, Lydia A. [Vis alle 18 forfattere av denne artikkelen] (2016). Effect of energy restriction and physical exercise intervention on phenotypic flexibility as examined by transcriptomics analyses of mRNA from adipose tissue and whole body magnetic resonance imaging. Physiological Reports. ISSN 2051-817X. 4(21). doi: 10.14814/phy2.13019. Vis sammendrag

  Overweight and obesity lead to changes in adipose tissue such as inflammation and reduced insulin sensitivity. The aim of this study was to assess how altered energy balance by reduced food intake or enhanced physical activity affect these processes. We studied sedentary subjects with overweight/obesity in two intervention studies, each lasting 12 weeks affecting energy balance either by energy restriction (~20% reduced intake of energy from food) in one group, or by enhanced energy expenditure due to physical exercise (combined endurance‐ and strength‐training) in the other group. We monitored mRNA expression by microarray and mRNA sequencing from adipose tissue biopsies. We also measured several plasma parameters as well as fat distribution with magnetic resonance imaging and spectroscopy. Comparison of microarray and mRNA sequencing showed strong correlations, which were also confirmed using RT‐PCR. In the energy restricted subjects (body weight reduced by 5% during a 12 weeks intervention), there were clear signs of enhanced lipolysis as monitored by mRNA in adipose tissue as well as plasma concentration of free‐fatty acids. This increase was strongly related to increased expression of markers for M1‐like macrophages in adipose tissue. In the exercising subjects (glucose infusion rate increased by 29% during a 12‐week intervention), there was a marked reduction in the expression of markers of M2‐like macrophages and T cells, suggesting that physical exercise was especially important for reducing inflammation in adipose tissue with insignificant reduction in total body weight. Our data indicate that energy restriction and physical exercise affect energy‐related pathways as well as inflammatory processes in different ways, probably related to macrophages in adipose tissue.

• Hjorth, Marit; Pourteymour, Shirin; Goergens, S W; Langleite, Torgrim Mikal; Lee, Sindre & Holen, Torgeir [Vis alle 11 forfattere av denne artikkelen] (2016). Myostatin in relation to physical activity and dysglycaemia and its effect on energy metabolism in human skeletal muscle cells. Acta Physiologica. ISSN 1748-1708. 217(1), s. 45–60. doi: 10.1111/apha.12631.
• Görgens, Sven W.; Hjorth, Marit; Eckardt, Kristin; Wichert, Stefan; Norheim, Frode & Holen, Torgeir [Vis alle 16 forfattere av denne artikkelen] (2016). The exercise-regulated myokine chitinase-3-like protein 1 stimulates human myocyte proliferation. Acta Physiologica. ISSN 1748-1708. 216(3), s. 330–345. doi: 10.1111/apha.12579. Vis sammendrag

  Aim: Chitinase-3-like protein 1 (CHI3L1) is involved in tissue remodelling and inflammatory processes. Plasma levels are elevated in patients with insulin resistance and T2DM. We recently showed that CHI3L1 and its receptor protease-activated receptor 2 (PAR-2) are expressed in skeletal muscle. Activation of PAR-2 by CHI3L1 protects against TNF- a -induced inflammation and insulin resistance. However, the effect of exercise on CHI3L1 and PAR-2 signalling remains unknown. The aim of this work was to study the impact of exercise on CHI3L1 production and the effect of CHI3L1/PAR-2 signalling on skeletal muscle growth and repair. Methods: Three human exercise studies were used to measure CHI3L1 plasma levels ( n = 32). In addition, muscle and adipose tissue CHI3L1 mRNA expression was measured in response to acute and long-term exer- cise ( n = 24). Primary human skeletal muscle cells were differentiated in vitro, and electrical pulse stimulation was applied. In addition, myo- blasts were incubated with CHI3L1 protein and activation of MAP kinase signalling as well as proliferation was measured. Results: Circulating CHI3L1 levels and muscle CHI3L1 mRNA were increased after acute exercise. In addition, CHI3L1 mRNA expression as well as CHI3L1 secretion was enhanced in electrically stimulated cultured myotubes. Incubation of cultured human myoblasts with CHI3L1 protein leads to a strong activation of p44/42, p38 MAPK and Akt as well as enhanced myoblast proliferation. Conclusion: Our findings suggest that CHI3L1 is induced by acute exer- cise and that CHI3L1/PAR-2 signalling activates myocyte proliferation, which is important for restructuring of skeletal muscle in the response to exercise training.

• Pourteymour, Shirin; Lee, Sindre; Langleite, Torgrim Mikal; Eckardt, Kristin; Hjorth, Marit & Bindesbøll, Christian [Vis alle 11 forfattere av denne artikkelen] (2015). Perilipin 4 in human skeletal muscle: localization and effect of physical activity. Physiological Reports. ISSN 2051-817X. 3:e12481(8), s. 1–15. doi: 10.14814/phy2.12481.
• Hjorth, Marit; Norheim, Frode; Meen, Astri Jeanette; Pourteymour, Shirin; Lee, Sindre & Holen, Torgeir [Vis alle 13 forfattere av denne artikkelen] (2015). The effect of acute and long-term physical activity on extracelluar Matrix and serglycin in huuman skeletal muscle. Physiological Reports. ISSN 2051-817X. 3:e12473(8), s. 1–19. doi: 10.14814/phy2.12473.
• Albrecht, Elke; Norheim, Frode; Thiede, Bernd; Holen, Torgeir; Ohashi, Tomoo & Schering, Lisa [Vis alle 12 forfattere av denne artikkelen] (2015). Irisin - A myth rather than an exercise-inducible myokine. Scientific Reports. ISSN 2045-2322. 5. doi: 10.1038/srep08889.
• Albrecht, Elke; norheim, frode; Thiede, Bernd; Lee, Sindre; Holen, Torgeir & Ohashi,, Tomoo [Vis alle 12 forfattere av denne artikkelen] (2015). Irisin – a myth rather than an exercise-inducible myokine. Scientific Reports. ISSN 2045-2322. doi: 10.1038/srep08889.
• Kanzleiter, Timo; Rath, Michaela; Görgens, Sven W.; Jensen, Jørgen; Tangen, Daniel S. & Kolnes, Anders Jensen [Vis alle 11 forfattere av denne artikkelen] (2014). The myokine decorin is regulated by contraction and involved in muscle hypertrophy. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 450(2), s. 1089–1094. doi: 10.1016/j.bbrc.2014.06.123. Vis sammendrag

  The health-promoting effects of regular exercise are well known, and myokines may mediate some of these effects. The small leucine-rich proteoglycan decorin has been described as a myokine for some time. However, its regulation and impact on skeletal muscle has not been investigated in detail. In this study, we report decorin to be differentially expressed and released in response to muscle contraction using different approaches. Decorin is released from contracting human myotubes, and circulating decorin levels are increased in response to acute resistance exercise in humans. Moreover, decorin expression in skeletal muscle is increased in humans and mice after chronic training. Because decorin directly binds myostatin, a potent inhibitor of muscle growth, we investigated a potential function of decorin in the regulation of skeletal muscle growth. In vivo overexpression of decorin in murine skeletal muscle promoted expression of the pro-myogenic factor Mighty, which is negatively regulated by myostatin. We also found Myod1 and follistatin to be increased in response to decorin overexpression. Moreover, muscle-specific ubiquitin ligases atrogin1 and MuRF1, which are involved in atrophic pathways, were reduced by decorin overexpression. In summary, our findings suggest that decorin secreted from myotubes in response to exercise is involved in the regulation of muscle hypertrophy and hence could play a role in exercise-related restructuring processes of skeletal muscle.

• Li, Yuchuan; Lee, Sindre; Langleite, Torgrim Mikal; Norheim, Frode; Pourteymour, Shirin & Jensen, Jørgen [Vis alle 13 forfattere av denne artikkelen] (2014). Subsarcolemmal lipid droplet responses to a combined endurance and strength exercise intervention. Physiological Reports. ISSN 2051-817X. 2(11). doi: 10.14814/phy2.12187.
• Norheim, Frode; Hjorth, Marit; Langleite, Torgrim Mikal; Lee, Sindre; Holen, Torgeir & Bindesbøll, Christian [Vis alle 13 forfattere av denne artikkelen] (2014). Regulation of angiopoietin-like protein 4 Production during and after exercise. Physiological Reports. ISSN 2051-817X. 2(8). doi: 10.14814/phy2.12109.

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• Fragoso Bargas, Nicolas; Linares-Pineda, Teresa; Molina-Vega, María; Picón, María José; Jenum, Anne Karen & Sletner, Line [Vis alle 14 forfattere av denne artikkelen] (2023). Methylation risk score for gestational diabetes on DNA methylation patterns associated with type 2 diabetes.
• Borgen, Tove Tveitan; Lee-Ødegård, Sindre; Eriksen, Barbara & Eriksen, Erik Fink (2023). Intermittent Dosing of Zoledronic acid Based on Bone Turnover Marker Assessment Reduces Vertebral and Non-Vertebral Fractures.
• Borgen, Tove Tveitan; Lee-Ødegård, Sindre; Eriksen, Barbara & Eriksen, Erik Fink (2023). Long term treatment with i.v. Zoledronic acid with dosing based on bone turnover markers.
• Elshorbagy, Amany; Bastani, Nasser Ezzatkhah; Lee-Ødegård, Sindre; Øvrebø, Bente; Haj Yasein, Nadia & Svendsen, Karianne [Vis alle 10 forfattere av denne artikkelen] (2023). Correction to: The association of fasting plasma thiol fractions with body fat compartments, biomarker profile, and adipose tissue gene expression (Amino Acids, (2022), 10.1007/s00726-022-03229-2). Amino Acids. ISSN 0939-4451. 55, s. 547–548. doi: 10.1007/s00726-023-03244-x.
• Fragoso-Bargas, Nicolas; Lee-Ødegård, Sindre; Moen, Gunn-Helen Øiseth; Oppsahl, JO; Sletner, Line & Jenum, Anne Karen [Vis alle 12 forfattere av denne artikkelen] (2022). Epigenome-Wide Association Study of objectively measured physical activity in pregnancy.
• Fragoso-Bargas, Nicolas; Lee-Ødegård, Sindre; Moen, Gunn-Helen Øiseth; oppsahl, JO; Sletner, Line & Jenum, Anne Karen [Vis alle 13 forfattere av denne artikkelen] (2022). Epigenome-Wide Association Study of objectively measured moderate physical activity in pregnan. Vis sammendrag

  Epigenome-Wide Association Study of objectively measured moderate physical activity in pregnancy Nicolas Fragoso-Bargas1,2, Sindre Lee-Ødegård2, Gunn-Helen Moen2,3,4,5, Julia O. Opsahl2, Line Sletner2,6, Anne Karen Jenum2, Paul Franks7, Rashmi B. Prasad8, Leif C. Groop8, Elisabeth Qvigstad1,2, Kåre I. Birkeland2, Kåre R. Richardsen9, Christine Sommer1 1Oslo University Hospital, Oslo, Norway, 2University of Oslo, Oslo, Norway, 3The University of Queensland Diamantina Institute, Brisbane, Australia, 4Norwegian University of Science and Technology, Trondheim, Norway, 5University of Bristol, UK, 6Akershus University Hospital, Oslo, Norway, 7Lund University Genetic and Molecular Epidemiology, Malmö, Sweden, 8Lund University Diabetes Centre, Malmö, Sweden,9Oslo Metropolitan University, Oslo, Norway. Physical activity (PA) is recommended during pregnancy to reduce the risk of excessive weight gain and gestational diabetes mellitus, and to improve general health. DNA methylation (DNAm) has been proposed as a potential molecular mechanism through which physical activity (PA) mediates the effects on the transcriptome. Here we performed an epigenome-wide association study (EWAS) of objectively measured moderate physical activity in pregnancy, which has not been performed previously. In EPIPREG (a sub-sample of the population-based STORK Groruddalen study) we quantified DNAm at gestational week 28 (±2 SD) with Illumina’s Infinium Methylation EPIC BeadChip. With SenseWear™ Pro3 Armband, we measured hours per day of moderate PA (3.0 to 6.0 metabolic equivalent for task (MET)) and steps/day. DNAm outliers that were 4 times the interquartile range below or above the 25TH and 75TH percentiles respectively were removed, before the beta values were transformed to M-values. Of the 472 that passed the EWAS quality control, 239 European (EUR) and 109 South Asian women (SA) had two valid days of recorded PA (19.2h per day), and were non-outliers (±4 SD) in their respective ethnic group. We used two approaches to test the association between M-values and moderate PA (hours/day): 1) A trans-ethnic approach using mixed linear models adjusted for blood cell proportions, age, smoking, number of steps, number of valid days (between 2 to 8), and ethnicity as a random intercept, and 2) through separate analyses in EUR and SA using linear modelling adjusted with the before mentioned covariates. We accepted a false discovery rate of 10%. In the trans-ethnic analysis, only cg08148261 located in the promoter area of LDHB was significantly associated with moderate PA. In Europeans, eight sites were significant: cg19031974 (BSN-AS2), cg19053287 (MCF2L) cg14930390 (MUTED), cg18517050 (CIAPIN1) and cg26191378 (TMUB1), located near promoter areas, cg27160968 (ESRRA) and cg15879426 (c17orf101) located in exon boundaries, and cg10421808 which is unannotated. In South Asians, only cg05999993 in the promoter area of AOAH was associated with moderate PA. Most of the sites showed decreased DNAm at increased moderate PA (hours/day), while in cg10421808, cg14930390 and cg15879426 the opposite was observed. In conclusion, we found several significant associations between DNAm and moderate PA. Several CpGs were located near gene promoter areas, which could imply altered transcription of the respective genes. These findings suggest that PA may exert changes in DNAm with important transcriptional implications during pregnancy.

• Lee-Ødegård, Sindre; Norheim, Frode Amador; Langleite, Torgrim Mikal; Gulseth, Hanne Løvdal; Birkeland, Kåre Inge & Drevon, Christian A (2022). Correction to: Effects of long-term exercise on plasma adipokine levels and inflammation-related gene expression in subcutaneous adipose tissue in sedentary dysglycaemic, overweight men and sedentary normoglycaemic men of healthy weight (Diabetologia, (2019), 62, 6, (1048-1064), 10.1007/s00125-019-4866-5). Diabetologia. ISSN 0012-186X. doi: 10.1007/s00125-022-05841-z.
• Lee-Ødegård, Sindre; Norheim, Frode Amador; Gulseth, Hanne Løvdal; Langleite, Torgrim Mikal; Aker, Andreas Helge Mikael & Gundersen, Thomas Erik [Vis alle 9 forfattere av denne artikkelen] (2018). Author Correction: Skeletal muscle phosphatidylcholine and phosphatidylethanolamine respond to exercise and influence insulin sensitivity in men. Scientific Reports. ISSN 2045-2322. 8(1). doi: 10.1038/s41598-018-26061-9.
• Eckardt, Kristin; Pourteymour, Shirin; Hjorth, Marit; Lee, Sindre; Langleite, Torgrim Mikal & Holen, Torgeir [Vis alle 9 forfattere av denne artikkelen] (2016). Dual specificity phosphatase 5 and 6 are oppositely regulated in human skeletal muscle by acute exercise.
• Eckardt, Kristin; Lee, Sindre; Langleite, Torgrim Mikal; Holen, Torgeir; Jensen, Jørgen & Birkeland, Kåre I. [Vis alle 7 forfattere av denne artikkelen] (2015). Stearoyl-CoA desaturase and its regulation in human skeletal muscle by exercise.

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