The Gözen Group's publication, "Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum" presents a convenient method to form a bottom-up structural organelle model for the endoplasmic reticulum (ER). The model consists of highly dense lipidic nanotubes that are, in terms of morphology and dynamics, reminiscent of ER. The networks are derived from phospholipid double bilayer membrane patches adhering to a transparent Al2O3 substrate.
The publication features a video of group leader, Irep Gözen, explaining the research, along with a video doctoral research fellow, Elif Köksal, demonstrating how to perform the method.
You can read the publication in full here: "Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum", Köksal, E. S., Belletati , P. F., Reint, G., Olsson, R., Leitl, K. D., Kantarci, I., Gözen, I. J. Vis. Exp. (143), e58923, doi :10.3791/58923 (2019).